Firma:                   Pharmacia

Modell:                P-950

Kommentar:        Dokumente engl.

Pump P-950 features:

•            Operating pressure up to 1.0 MPa (10 bar, 145 psi).

•            Flow rates up to 50 ml/min.

•            A pressure sensor connected to the pump outlet.

Description

Pump P-950 is a single-channel laboratory pump for use as a sample pump in ÄKTAdesign liquid chromatography systems.

An eluent in an external vessel is drawn into three liquid chambers in the pump. A stepper motor assembly performs the pumping action of the chambers. This assembly acts on the chambers in a sequential order, which gives a smooth flow from the pump

The pressure generated by the sample pump is continuously monitored by the pressure transducer housed in the pressure sensor housing. The signal, which is proportional to the pump pressure, is read by UNICORN via the UniNet-2 connection.

Cable connections

The pump has two connectors on the bottom side for connection to the UniNet-2 communication network. This enables control of the pump from UNICORN, version 3.2 or higher, via Pump P-900 or Pump P-920. The UniNet-2 Gable also supplies power to the pump.

Liquid delivery

Pump P-950 has one inlet and one outlet connected to three liquid chambers. The chambers haue flexible membranes which do the actual pumping of the liquid. The membranes are actuated by pistons driven by a rotating stepper motor assembly. This gives a sequential Operation on the chambers, which results in a smooth and continuous liquid delivery.

Each of the three liquid chambers has a flexible membrane and two holes for inlet and outlet. The inlet to each chamber is fed from the pump inlet. A rotating manifold ensures that only one chamber inlet is connected to the pump inlet at a time. The membranes are actuated by pistons in a sequential order – from completely pressed out to fully retracted. Hence, one chamber will draw solvent while the second is expelling solvent and the third is about to starr drawing or expelling.

When a chamber is full, the rotating manifold closes the chamber inlet and opens the chamber outlet instead. Therefore, no check valves are required. At the same time, The piston starts to press on the chamber membrane. This forces the solvent out from the chamber and through the pump outlet. On the way out, the solvent passes the pressure sensor housing where the pressure is measured.

The sequential pumping action of the pistons is provided by a stepper motor through an axial bearing. The bearing is slightly tilted, which creates a reciprocal motion on the pistons during the rotation.

The stepper motor has automatic speed control to reduce pulsation.

Technical specifications

Operating data

Flow rate range                       0.1-50 ml/min in steps of 0.1 ml/min

Pressure range                       0-1.0 MPa (10 bar, 145 psi)

Flow rate accuracy                 ±3% or ±0.1 ml/min whichever is greater

Flow rate reproducibility          rsd < 2% or ±0.1 ml/min whichever is greater

Viscosity                                  Max. 5 cP for complete flow range. At reduced flow rate (≤ 10 ml/min): max. 10 cP.

Pressure sensor

scale error                               Max. ±2%

Offset error                              Max. 0.01 MPa/week

Physical data

Tubing connectors

pump inlet                                male UNF 5/16"

pump outlet                             male UNF 10-32

pressure sensor inlet              male UNF 10-32

pressure sensor outlet            male UNF 10-32

Internat volume                        1.1 mi including pressure sensor housing

Control                                     Via UniNet-2 Gable connection

Degree of protection               IP 21

Wetted materials

liquid chambers                       Simriz, PEEK

pump outlet and inlet               Polyethene, titanium

rotating manifold                      Ceramic, Kalrez

pressure sensor outlet            PEEK

and inlet

pressure sensor                      titanium

Chemical resistance               The wetted parts are resistant to the chemicals listed below (synergistic effects haue not been taken into account; room temperature and limited over-pressure is assumed).

                                               Unless otherwise stated, all concentrations are 100%:

                                               •         Acetic acid, 0.1 M

                                               •         Acetone, 1

                                               •         Aqueous buffers, pH 2-12

                                               •         Decon 90, 10% (for washing only)

                                               •         Ethanol, 20%

                                               •         Ethanol, 96% (for washing only)

                                               •         Ethylene glycol

                                               •         Formic acid, 1%

                                               •         Guanidine, 6 M

                                               •         HCI, 0.1 M

                                               •         Isopropanoi, 30%

                                               •         Lysozyme, 2 mg/ml

                                               •         Methanol, 20%

                                               •         NaOH, 0.1 M

                                               •         NaOH, 1 M (for washing only)

                                               •         SDS, 10% (short term use)

                                               •         TFA, 0.2%

                                               •         Triton-X, 2% (short term use)

                                               •         Urea, 8 M

Power requirement                 32 V DC

Environment                            +4 to +40 °C

                                               10-95% relative humidity

                                               84-106 kPa (840-1060 mbar)

Dimensions, H x W x D          120 x 100 x 140 mm

EMC standards                       This product meets the requirements of the EMC Directive 89/336/EEC through the harmonized standard IEC/EN 61326-1 (emission and immunity).

          Note: The declaration of conformity is valid for the Instrument if it is:

                                               •         used in laboratory locations

                                               •         used in the saure state as it was delivered from Amersham Pharmacia Biotech except for alterations described in the User Manual

                                               •         connected to other CE-Iabelled Amersham Pharmacia Biotech modules or other products as recommended.