Firma:              Waters

Modell:                         Quanta 4000

Kommentar:     Dokumente engl.

Application Specific Systems

Quanta ^TM 4000 Capillary

Electrophoresis System

Waters new Quanta 4000 Capillary Electrophoresis system is an affordable automated CE system with high sensitivity, Iow-noise UV/Visible detection, integrated fraction collection, system temperature stabilization, and two modes of automatic injection for flexibility.

Waters Quanta 4000 offers a plug-in program card for easy software upgrades and is compatible with Waters data workstations.

Separation

Number of samples                Up to 20

Sampling modes                     Hydrostatic and electromigration

Hydrostatic injection                9.8 cm head

Electromigration (Sample       Programmable 0-30 kV/sample

Voltage)

Repetitions                             Up to 9 injections per vial, programmable by sample number

Sample Time                           0 to 999 seconds, programmable by sample number

Separation Time (Run Time) 0 to 199.0 minutes, programmable by sample number

Separation Voltage (Run         Programmable 0-30 kV/sample

Voltage)

Polarity                                    Positive or Negative high voltage power supply, replaceable by user

Injection volume                      RSD less than 2% with internal standard

reproducibility

Migration time reproducibility   RSD less than 1% with internal standard

Filling mechanism                   Vacuum applied at collection end 10 to 15 inches of Hg

Filling/cleaning solutions        3 off-line vials installed next to carousel. Operators choice of solutions

Operating temperature            10 to 40 °C

Operating humidity                  20 to 80% relative humidity, non-condensing

Detection

Type                                        Fixed wavelength UV absorbance

Wavelengths                           185, 214, 229, 254, 280, 313, 365, 405, 436, and 546 nm. Change wavelength by changing filier, window, or Iamp.

Noise level                               Less than 5 x 10 ^-5 AU, peak to peak. Time Constant = 1.0

Drift                                          1 X 10 ^-3 AU/hour

Linear dynamic range             From 1 mg/mL to 1 µg/mL of the peptide Neuromedin

Sensitivity settings                  0.5, 0.2, 0.1, 0.05, 0.02, 0.01, 0.005, 0.002, 0.001 AUFS

Time constant                         0.1, 0.3 and 1.0 second

Capillary mount                       Self-aligning mount

Analog outputs                        10 mV recorder

                                               1 AU / V integrator

Electrical

External Inputs                        Start, Reset (contact closure or TTL active low)

Outputs                                   Inject start contact closure; TTL compatible; 50 mV at 100 mA, maximum

10 mV full scale (set by Range select knob) chart recorder outputs

0-2 V @ 1 V/AU absorbance output (not affected by Range select knob)

Capillary-current analog 0 to1 V=0 to1 mA, approximately 250 µA, maximum

                                               Temperature analog 0 to1 V=0 to100°C (10 mV/°C)

Power requirements               90 - 132 V, 50 or 60 Hz, or

                                               198 - 264 V, 50 or 60 Hz

Blackout tolerance                  40 msec, minimum

Power-failure behavior            The System automatically resets itselt to its initial power-on state.

Capillary

Material                                    Fusel silica coated with poiyimide

I.D.                                           10 to 200 µm

O.D.                                         363µm ± 12µm

Length                                     30 cm to greater than 100 cm

High Voltage Power Supply

Output                                     0 to 30 kVDC, 250 µA adjustabie in 10 V steps

Lowest usable voltage            200 VDC

Polarity                                    Two configurations: Positive or Negative ground