Firma:                   Waters

Modell:                M 484

Kommentar:        Dokumente engl.

The WatersTM Model 484 Tunable Absorbance Detector (referred to as the Waters 484) is a single-channel tunable ultraviolet/visible (UVNIS) detector designed for high performance liquid chromatography (HPLC) applications.

The Waters 484 monitors the eluent stream from a chromatographic column and detects the presence of sample molecules through optical absorbance at a wide range of specific wavelengths in the ultraviolet and visible spectrum. lt has a usable wavelength spectrum of 190-600 nanometers (nm) although its primary use is in the ultraviolet wavelength range between 190 and 380 nanometers. The maximum sensitivity of the instrument is .001 absorbance units full scale (AUFS).

The Waters 484 can operate as a "stand-alone" unit (with a chart recorder) or with other Waters System modules, such as the Waters 745 integrator.

The Waters 484 offers the following features:

•     A modular design that allows for easy servicing

•     Extensive self-calibration to ensure wavelength accuracy and diagnostic routines to verify    that the Waters 484 is functioning correctly

•     Battery backup to retain parameter settings when the detector is powered down or in the      event of power interruptions

•     A deuterium arc lamp

•     The patented Taper-Cell ^® flow cell design which reduces refractive index interference           from the absorbance signal

The main components of the Waters 484 include the mechanical parts (such as the front and rear panels and the outer top cover), optics, electronics, and the software.

The optics consist of the optical source and other necessary components that allow the detector to perform absorbance detection.

The electronics of the Waters 484 are digital-based and include hardware such as a front panel keyboard, printed circuit boards and their interconnections. The electronics also include software to control operations such as sensitivity settings, wavelength selection and calibration, and the diagnostics of the detector.

Operation – Overview

The Waters 484 is simple to operate. By pressing appropriate keys on the keyboard of the front panel, you access parameters (such as absorbance, sample energy, reference energy, or wavelength) and enter values for specific parameters.

The Waters 484 mechanical components include the front and rear panels and the outer top cover.

The right siele of the front panel consists of the keyboard, the displays and indicators, and the ON/OFF rocker switch.

The left side of the front panel includes the access panel which holds the fluid connections. The flowcell and drip tray are located below the access panel.

All electrical connections and the fuse and AC receptacle are located an the rear panel. The rear panel may include the optional IEEE-488 interface board.

Be sure you have properly installed the Waters 484 before operating. Refer to Section 2.7, Installation Summary.

For normal use, be sure to allow the Waters 484 to run for thirty minutes before operating.

Mechanical Specifications

Height                                                9.2 inches (24 cm)

Length                                               19.5 inches (49.5 cm)

Width                                                11.2 inches (28.5 cm)

Weight                                              28 pounds (13 kg)

Environmental Specifications

Operating Temperature                    15 - 32.2°C

Operating Humidity                           20 - 80%, non-condensing

Storage Temperature                       40 - 65°C

Shipping and Storage Humidity        20 - 80% non-condensing

Operational Specifications

Wavelength Range                           190-600 nm

                                                         (single, fixed wavelength)

Wavelength Accuracy                      ±2 nm

Wavelength Repeatability                 ±0.25 nm

Noise                                                 3.0 x 10 ^-5 AU (peak-to peak, 1 second filter setting, dry         cell, 254 nm)

Drift                                                   2.5 x 10 ^–4 AU with ambient temperature change of less        than 1°C per hour

Sensitivity Setting Range                  0.001 - 2.0 AUFS (under software control)

Filter Setting Range                          0.1 - 99.0 seconds (under software control)

Analog outputs                                  1.0 V/AU (unattenuated)

                                                         Integrator Channel

                                                         10 mV Full Scale Recorder Channel

                                                         Reference energy recorder channel (attenuated)

Auto Zero                                          Activated through the front panel or remote event input         (under software control)

Chart Mark                                        Activated through the front panel key or remote event           input

Polarity                                              Activated through the front panel key (10 mV Full Scale        Output)

Event Inputs                                      Remote start inject and Auto Zero

Front Panel Displays                        4 digit 7-segment LEDs (absorbance and energy)

                                                         3 digit 7-segment LEDs (parameters)

                                                         Remote LED Indicator (IEEE-488)

Operational Specifications (cont'd)

IEEE-488 Bus                                   Operational with IEEE-488 Interface Assembly

IEEE-488 Controllable Parameters Wavelength, Filter Setting, Auto Zero, Chart Mark,     Polarity, Sensitivity

Lamp Source                                    Deuterium arc lamp

Flowcell                                             Dual beam Taper-Cell design

Flowcell Path Length                        10 mm (standard, analytical)

Flowcell Pressure Limit                    1000 psig

Flowcell Materials                             316 stainless steel, Teflon, Suprasil Il

Electrical Specifications

Power Requirements                       Setting                  Range

                                                         100 Vac                85 - 110 Vac

                                                         120 Vac                102 - 132 Vac

                                                         220 Vac                187 - 242 Vac

                                                         240 Vac                204 - 264 Vac

Line Frequency                                 50 Hz                    47 - 53 Hz

                                                         60 Hz                    57 - 63 Hz

Fuse Ratings                                    1.5A, 100/120 VAC

                                                         0.75A, 220/240 VAC

Power Consumption                         100 VA (Nominal)

DC Power Supply                             +5VDC at 2.2 A (Power limited to 11W)

                                                         +/- 15VDC at 300 mA

Lamp Power Supply                         309mA Regulated Constant Current (Fused at 3/8 A)

                                                         3.0VAC Filament Heater

                                                         (unregulated (Fused at 6 1/4 A)

                                                         500 VDC Arc Strike (nominal)

Optical Specifications

Monochromator                                Seya-Namioka configuration

Grating                                              Concave holographic

Optical Bandwidth                            8nm

Lamp Power                                     30W