Firma:              Tepnel

Modell:            DARAS

Kommentar:   Dokumente engl.

1.          Micro-Syringe Pump

2.          Door

3.          Pinch Valve

4.          Waste Pump

5.          Buffer Bottles

6.          Platten Position Indicator

7.          Waste Bottle

B.         Power On/Off Switch

9.          Drip Tray

10.        Wash Station

11.        Reaction Column Holder

12.        Manifold

13.        Door Lock Indicator

14.        Power On Indicator

15.        Platten

16.        Column Head Assembly

17.        Column Heads

18.        Heater Block Assembly

19.        Optical Sensors

20.        Photometer

21.        Adjustable Feet

22.        Lower Casing

23.        Lower Casing Release Button

24.        Reagent Trays

WHY CHOOSE DARAS

As researchers move progressively towards unravelling the genetic code, our ability to understand and manipulate DNA continues to advance at a rapid rate. To date, traditional methods of DNA and RNA analysis have failed to keep pace with the demands for automation and high throughput analysis. Furthermore, many laboratory techniques are still labour intensive, requiring high levels of technical involvement, with the results being open to subjective interpretation. The automation of complex molecular biology analyses will reduce technician involvement in routine procedures whilst producing consistent results within today's busy laboratory environment. To address these issues, DARAS has been specifically developed as a fully automated system designed to carry out all the experimental steps associated with complex molecular biology analyses.

The DARAS automated system combines the amplification potential of the polymerase chain reaction (PCR), with patented sequence-specific capture and detection of nucleic acids. Manipulation and detection of PCR products using the automated technology, provides accurate detection at low DNA input levels whilst eliminating the inherent technical problems associated with the interpretation of conventional gel-based separation and analysis methods. All critical reactions are under precise temperature control and are performed in disposable Reaction Columns carrying target-specific micro-beads.

The modular instrument system is fully automated under the control of a multi-tasking surveillance computer, allowing data collection and full sample analysis and traceability. The DARAS system gives users the opportunity to automate and simplify the detection of DNA products, allowing rapid, reliable analysis of multiple samples. DARAS is capable of analysing up to 100 DNA samples per day, using pre-programmed protocols, each taking approximately 30 minutes, giving users the assurance of increased sensitivity and high specificity.

Computer Specifications

•     PC with 80486, or higher processor

•     Serial COM port

•     8 MB RAM, or higher

•     At least 4 MB free disk space on your hard drive

•     SVGA Monitor

•     Microsoft Windows 3.11, or higher

Connect the instrument to the computer via the COM port using the RS232 communication cable provided.

Technical Specification

TYPE OF INSTRUMENT

Multi-analysis, compact automated analyser for specific DNA or RNA target sequences.

TYPE OF ASSAY

Customer-defined or analyse-specific DNA probe technology.

LINKAGE CHEMISTRY

Covalent linkage to micro-bead via 5'-amino oligonucleotide.

ASSAY FORMAT

Single-use disposable Reaction Column and 96 well reagent plates.

NUMBER OF SAMPLES

Individual selection of up to 12 sampes/run. Electronic confirmation of Column attachment.

SAMPLE THROUGHPUT

Detection protocol of under 30 minutes, with operator `hands-on time of less than 5 minutes.

SAMPLE VOLUMES

Minimum sample volume 50µL DNA concentration 0.1 - 10ng/50µL.

FLUID PATHWAY

Inert fluid pathway of Teflon, Polypropylene and 0.8 mm ID silicon rubber tubing.

FLUID HANDLING

Capable of mixing, diluting, pipetting, aspirating and dispensing over a range of 1- 100µL ± 1 µL.

REAGENT FORMAT

Ready to use, sterile, single-use buffers and reagents. Buffer selection from two positions via software.

BUFFER/WASTE BOTTLES

2 x 50 mL Buffer bottles, 1 x 250 mL. Waste bottle, with easy release/push-fit connection.

TEMPERATURE RANGE

Enclosed foil heating and Peltier cooling operating between + 30°C to 98°C ±0.5°C.

HEATING PERFORMANCE

Greater than 1°C/second.

DETECTION SYSTEM

Alkaline phosphatase enzyme amplification of biotin-labelled nucleic acids.

PHOTOMETER

Photometric detection at 492nm providing Kinetic or End-point determination.

DARAS ^© products may be suitable for use in certain processes which may be covered by patents or patent applications owned by third parties. Purchase of any DARAS product does not convey a license to use such processes. Persons wishing to perform such processes must obtain a license from the proprietor.