Manufacturer:             Tecan

Model:                         Spectrafluor pl

The SPECTRAFLUOR PLUS is a fully automatic, computer controlled fluorometer for measuring samples in a microplate. The instrument fulfills the requirements for numerous fluorescence, absorbance and glow type luminescence applications in research and routine investigations.

The filters are arranged in filter slides; four excitation filters and four emission filters respectively. The instrument has no on-board software and is exclusively controlled via an external computer using Windows^TMformat.

1 = power LED

2 = emission filter

3 = excitation filter

4 = plate carrier

Instrument Features

General

The following types of measurement are provided with the SPECTRAFLUOR PLUS microplate reader.

Measurement Type             Read Mode                           Microplates to be Used

Fluorescence                         Standard and time-                6 - 1536 wells

                                               resolved, top and bottom

Absorbance                            Single and dual                       ≤ 384 wells

                                               wavelength

Glow type luminescence                                                       96 wells

The table below lists the technical specifications for the instrument.

Parameters                           Characteristics

Measurement                         Software controlled

Interface                                 Serial interface: RS 232

Filter handling                         External filter exchange

                                               4 excitation filters and 4 emission filters in separate filter slides.

                                               Up to 8 excitation and emission filter slides can be defined            when using software.

Plate definition                        Via scanning software

Temperature control              From ambient +5°C up to 42°C

Plate shaking                          Orbital or linear shaking - both offer 3 speeds

Light source                           High energy Xenon flash lamp

Optics                                     Quartz fibers and lenses

Detector                                 Side window, low dark current photomultiplier tube

Power supply                         Auto-sensing:100-120V / 220-240V 50/60 Hz

Power consumption               200 VA

Main fuse                                T 2A / 250 (slow blow)

Physical

Outer dimensions                  Width: 380 mm

                                               Depth: 415 mm

                                               Height: 170 mm

Weight                                    18.8 kg

Environmental

Ambient temperature

Operation                               15°C –30°C    59°F- 86°F

Non-operation                         -20°C -+60°C-4°F - +140°F

Relative humidity

Operation                               90% non-condensing

Overvoltage category             II

Pollution degree                     2

Usage                                     Commercial

Noise level                              < 70 dBA

Method of disposal                 Electronic waste (infectious waste)

Fluorescence

Parameters                   Characteristics

Sensitivity                      ≤ 1.5 pg fluorescein / well (200 µl / well) or ≤ 4 fmol / well

                                      in a Greiner 96 well black plate using 100 flashes / well

Wavelength Range       230 - 700 nm

Standard Filters            Wavelength         Band Width¹⁾

Excitation                      1. 360nm             35nm

                                      2. 430nm             35nm

                                      3. 485nm             20nm

                                      4. 590nm             20nm

Emission                       1. 465nm             35nm

                                      2. 535nm             25nm

                                      3. 595nm             35nm

                                      4. 635nm             35nm

Read Mode                   Top and bottom (bottom for transparent plates)

Integration Time²⁾          20 - 2000 µs

Lag Time²⁾                    0 - 2000 µs

1) Band width for standard filters may have some tolerance ±10%

2) Needs to be adjusted for Time Resolved Measurements. Lag Time is typically ≥ 40µs

Gain setting                     Values                     Measurement range

Manual                               1 -255                       0 - 60,000 RFU

Optimal                              Automatic                 0 - 60,000 RFU

Extended Dynamic            Automatic                 0 - 6,000,000 RFU

Range                                                                 (using XFLUOR)

No. of                    Definition                                Characteristic             Meas. Time for Flashes/ Well                                                                                        96 Wells

1 - 3                        Plate carrier does not stop       max. speed                   ≤ 30 sec

                               at the measurement point

                               (on the fly)

4-255                      Plate carrier stops at the          max. precision              Dependent on the      meas. point                                    flash parameter                                                                         setting

Absorbance

The specifications only apply to 96 well plates.

Absorbance can not be measured in 1536 well plates.

The absorbance filters are not within the instrument's standard configuration.

Parameters                  Characteristics

Wavelength range        230 - 750 nm

Meas. range                  0-3 OD

Resolution                     0.0001 OD

Accuracy                       0 - 2.0 OD    ± 2.0 % and ± 0.010 OD        492 nm

Precision                       0 - 2.0 OD    ± 2.0 % and ± 0.005 OD        492 nm

Linearity                        0 - 2.0 OD    ± 2.0 %                                   492 nm

                                      0-1.5 OD      ± 2.0 %                                   260/340 nm

Standard filters             405 nm / 450 nm / 492 nm / 620 nm

                                      (not included in standard delivery)

Glow Type Luminescence

The specifications only apply to 96 well plates.

Luminescence can only be measured in 96 well plates.

Parameters                  Characteristics

Sensitivity                      < 0.4 10^-6DEA activity units/well of molecular biology grade alkaline       phosphatase (20 µl/well) using CDP-Star (Tropix) chemiluminescent            substrate mix with Emerald-II enhancer (100 µl/well) in a Greiner 96                     well white plate. Use of the cover plate greatly reduces the cross-talk.

                                      0.4 10^-6DEA activity units/well correspond to 200 fg/well of alkaline        phosphatase when using a lot of 2000 DEA activity units/mg protein.

Wavelength range        400 - 700 nm

Gain                              Optimal:       preset value

                                      Manual:         1 -255

Integration setting               Integration time                    Measurement range

Manual                                    1 - 5000 ms                            0 - 55,000 RLU

Maximum Dynamic                automatic (1 - 1000 ms)        0 - 55,000,000 RLU

Range                                                                                    (using XFLUOR)