Firma:              Nova

Modell:            Bioprofile 200

Kommentar:   Dokumente engl.

Two Multi-Test Analyzers

Provide Fast, Easy Measurements

BioProfile analyzers are fully automated, multi-test systems designed for the rapid analysis of key nutrients, metabolites, and gases in cell culture media.

Features of the BioProfile include:

• Up to twelve key parameters simultaneously measured including: glutamine, glutamate, glucose, lactate, pH, pO ₂ , pCO ₂ , ammonium, sodium, potassium, calcium, and cell density.

• All results available in as little as 2 minutes

• Automatic one-button operation

• 0.5 mL sample size

• Direct sampling from syringes, tubes, flasks, and pipettes

• No sample dilution or centrifugation

• Automatic analyzer calibration

Two Test Menus

The BioProfile test menus provide those assays necessary to address the testing requirements of research and development laboratories, and pilot production facilities. Developed in conjunction with leading biotechnology companies, these test menus enable control of the most critical constitu­ents of cell culture media.

Applications of the BioProfile include:

• Monitoring the progress of bioreactor runs

• Determining the consumption and production rates of key metabolites

• Identification of growth-limiting nutrients

• Design of cell culture feeding strategies

• Calibration of bioreactor probes

• Measurement of cellular respiration

• Balancing media electrolytes

• Controlling levels of waste products

Fast, Comprehensive Testing Gives

Real-Time Results and Reduces Costs

BioProfile's comprehensive test menus allow con­solidation of testing in a single analyzer. This results in significant time and labor savings, as well as real-time process control capabilities. As shown below, BioProfile analyzers can replace up to seven differ­ent instruments and measurement techniques.

BioProfile's fast analysis time and consolidated testing:

Eliminates the requirement for taking multiple samples and large sample volumes from reactor vessels

• Provides real-time access to test results, enabling real-time process control

• Decreases labor, capital equipment, and reagent costs by 75%

• Improves the ability to effectively monitor and control bioreactor processes

• Improves process reliability and reproducibility

• Speeds optimization of cell culture feeding strategies

• Improves manufacturing yield and quality

Instrument Specifications

Measurement Range:                      Na ⁺                             40 - 220 mmol/L

                                                        K ⁺                               1.0 - 25.0 mmol/L

                                                        ICa                              0.2 - 10 mmol/L

                                                        NH 4 ⁺                           0.2 - 25 mmol/L

                                                        PH                              5.00 - 8.00

                                                        P CO ₂                         3.0 - 300 mmHg

                                                        P O ₂                            0 - 800 mmHg

                                                        Glucose                      0.2 - 15.0 g/L

                                                        Lactate                       0.2 - 5.0 g/L

                                                        Glutamine                   0.2 - 6.0 mmol/L

                                                        Glutamate                  0.2 - 6.0 mmol/L

Measuring Technology:                    Ion Selective Electrodes (Na ⁺ , K ⁺ , iCa, NH ₄ ⁺ , pH, P CO ₂ )

                                                        Amperometry ( P O ₂ , Glucose, Lactate, Glutamine,     Glutamate)

Analysis Rate:                                  Mode                         Analysis Time        Throughput Time

                                                        Single Mode               3.9 minutes             4.2 minutes

                                                        Normal Mode             1.9 minutes             2.2 minutes

                                                        •    Analysis time measures how long the analyzer takes togive results.

                                                        •    Throughput includes washout time and measures how long it takes before the analyzer is ready for the next sample.

Sample Volume:                              500 microliters

Barometer:                                       450 - 800 ± 2 mmHg

Reagents:                                        A single reagent pack contains all fluids for 300 samples

                                                        (2700 tests)

Slope Limits:                                    pH                               9.1 - 11.2

                                                        P O ₂                            -15.0 - (-1.6)

                                                        P CO ₂                         7.9 - 12.2

                                                        Na ⁺                             8.8 - 10.7

                                                        K ⁺                               8.6 - 10.9

                                                        ICa                              8.9 - 13.0

                                                        NH 4 ⁺                           8.6 - 14.0

                                                        Gluc                            5 - 50

                                                        Lac                             6 - 80

                                                        Gln                              5 - 60

                                                        Glu                              5 – 60

Analytical Specifications for Imprecision

Analyte            Within-run                                                 Day-to-Day

                        Precision                                                  Precision

                        n=20                                                           n=20

                        Whichever is Greater                                 Whichever is Greater

                        CV%               SD                                        CV%                 SD

PH                                          0.005                                                            0.018

P CO ₂               5.0                  1.0 (mmHg)                          7.0                    2.8 (mmHg)

P O ₂                  5.0                  1.5 (mmHg)                          7.0                    4.2 (mmHg)

Na ⁺                   1.5                  1.0 (mmol/L)                        2.1                    1.4 (mmol/L)

K ⁺                     3.0                  0.15 (mmol/L)                      4.2                    0.21 (mmol/L)

ICa                    4.0                  0.05 (mmol/L)                      5.6                    0.07 (mmol/L)

NH ₄ ⁺                 5.0                  0.10 (mmol/L)                      7.0                    0.14 (mmol/L)

Gluc                  5.0                  0.03 (g/L)                              7.0                    0.07 (g/L)

Lac                   5.0                  0.03 (g/L)                              7.0                    0.07 (g/L)

Gln                    5.0                  0.10 (mmol/L)                      7.0                    0.14 (mmol/L)

Glu                    5.0                  0.10 (mmol/L)                      7.0                    0.14 (mmol/L)

Calibration Gases:                           The 2 gas cylinders are “E” size and contain gases as         follows:

                                                        Gas Standard A        Specified Value            Accuracy

                                                        Carbon Dioxide          5.0%                               ± 0.03%

                                                        Oxygen                       20.0%                             ± 0.03%

                                                        Nitrogen                      Balance                          —

                                                        Gas Standard B       Specified Value            Accuracy

                                                        Carbon Dioxide          10.0 %                            ± 0.03%

                                                        Nitrogen                      Balance                          —

Electrical Compliance:                     To meet UL and CSA standards

Temperature Thermostatting:          37°C ± 0.1°C

Dimensions:                                     Height:     18.75 in (46.1 cm)

                                                        Width:      22.25 in (56.5 cm)

                                                        Depth:     23 in (58.4 cm)

Weight:                                             70 lb (52.38 kg) without reagent pack

Power:                                              90-264 VAC, 50/60 Hz, 200W

Cleaning the CRT:                           Clean the display screen with a damp (not wet) lint-free cloth.

                                                        For a heavy buildup, use a liquid glass cleaner sprayed onto a lint-free cloth first, never spray directly onto the CRT display.

Cleaning the Cabinet:                      Clean the cabinet with a damp (not wet) lint-free cloth. Do not use aerosol sprays, solvents, or abrasives that might damage the finish.