Firma:              Intracellular

Modell:            InCyt Im2

Kommentar:   Dokumente dt. (nur für IDNR. 10555)

Ein schlüsselfertiges System zur Messung intrazellulärer Ionenkonzentrationen (z.B.: Ca ²⁺ , Na ⁺ , Mg ²⁺ , pH etc.).

Es besteht aus folgenden Komponenten:

·          Imaging Workstation:

PC’s:

Intel Pentium III,

Windows NT, 500 Mhz

Data Translations 3155 Board

256 MB RAM

20 GB HD

internes ZIP-Laufwerk (250Mb)

1.44 MB 3,5" Floppy Drive

CD-ROM-Laufwerk

Graphikkarte

17" SVGA Monitor

·          Lichtquelle:

300 W Xenon-Lampe (regelbare Helligkeit)

·          High Performance Digital CCD Camera

COHU Cooled Low-Light Level CCD-Kamera:

Auflösung: 640x480 Pixel

·          Filterrad:

Bestückt mit Anregungsfilter für FURA-2-Messungen (340 nm und 380 nm)

·          Flüssigkeitslichtleiter

·          Nikon Eclipse TE 300

Inverses Routinemikroskop mit CFI60-Unendlich-Optiksystem:

-           CFI Plan Fluor 10x

-           CFI S-Fluor 20x

-           CFI S-Fluor 40x

InCyt TM Im2 Software für Datenaufnahme und Analyse

Applications for InCyt systems include:

•            Intracellular measurement of Ca ²⁺ , pH, cyclic ADP Ribose, H ₂ 0 ₂ , Na ⁺ , K ⁺ , Mg ²⁺ , etc.

•            Automated uncaging of caged molecules.

•            Video calipers for microvessel diameter measurement.

•            Morphometry, object counting, and image enhancement.

•            Automated edge detection.

•            Simultaneous Ca ²⁺ and forte transduction.

Key system features:

•     Complete, turnkey systems - including microscope, computer, illumination source, camera or photometer, and software. All components are designed to work together from the start, so there's no guesswork. Our systems can also be integrated with most fluorescence microscopes.

•     A choice of systems - microscope-based imaging, microscope-based photometry, combination imaging and photometric microscopy, and cuvette-based photometry, each with single- and dual-wavelength options. We also have hardware and software add-on modules for specific application needs.

•     Easy-to-use InCyt software - Everyone claims ease-of-use...we deliver. At our installation & training session, you will run your first experiment on your own! Set-up, execution, and data analysis are fast and easy because the user interface guides you through the experiment in a logical and intuitive manner.

•     Modular design - for easy upgradability, expansion, and switching among detection modes, sample types, or filter sets.

•     Powerful processing system - including a Pentium Pro PC and Windows NT operating system for true 32-bit image processing and rapid image analysis.

•     Faster data collection - New filter changer and software allow you to capture data at double the rate of our earlier systems.

•     All this at a truly economical price! Add fluorescence analysis to your lab today!

•     Specify up to 50 regions of interest for separate analysis.

•     Analyze data during the experiment or save images for post-hoc analysis.

•     Image averaging for noise reduction.

•     Custom-design your palette for converting grayscale images to color.

•     Publish results more easily with InCyt's montage and annotation software - or export .tif and ASCII files to any popular spreadsheet or presentation package.

•     Play back images in animation.

•     Available in single- or dual-wavelength models.

Single- vs. Dual-Wavelength

The dual-wavelength, or ratio, mode has emerged as the method of choice for many investigators studying intracellular calcium and pH, because it measures absolute ion concentrations in cells. By rationing the fluorescence intensity at two different wavelengths, the investigator can remove the effects of variables such as cell volume, concentration of dye, etc. (Dual-wavelength models all end in "2," such as InCyt lm2''.)

Video Imaging vs. Micro- Photometry

Video Imaging

The main advantage of imaging is spatial resolution. Choose imaging if your studies involve analyzing populations of cells with heterogeneous responses, subcellular gradients, or intercellular waves. Imaging allows the investigator to see exactly where in each cell the response occurs.

Photometry

Photometry is superior to imaging in both temporal resolution and Iight sensitivity. However, photometry provides no spatial resolution - the total response from the selected field is captured as a single value at each time point. Photometry may be best for studies of extremely brief responses (such as calcium transients in neurons), studies in which the object is moving (such as a contracting muscle), or studies on cells that are particularly sensitive to photodamage.

Microscopy vs. Cuvette

Microscopy

Microcopy techniques are appropriate for research in which you wish to study a single cell or group of cells which can be brought into focus.

Cuvette

Many investigators require the flexibility of measuring fluorescence in cell suspensions as well as adherent cells on coverslips. For these investigators, a cuvette-based macro system may be the most appropriate.

The cuvette holds either suspended cells or a coverslip in the excitation Iight path while a photometer captures the fluorescent response of the population. (Cuvette systems have model names beginning in "Cv.")