manufacturer :       BMG

model :                   Fluostar Galaxy

annotation :           Dokumente engl .

Introduction

The FLUOstar galaxy is a fluorescence microplate realer that can perform fluorescence Intensity and time-resolved fluorescence

lt is designed for the widest possible range of non-radioactive labelled applications:

•            Ca ²⁺ - measurements (e.g. Ca ²⁺ Flux with Fura-2 or Inclo-1)

•            Enzyme activity

•            Cell toxicity, proliferation and Viability

By integrating a high intensity xenon flash lamp and liquid light guide technology the FLUOstar galaxy can cover the wavelength range from 240 to 740 nm. The FLUOstar galaxy can read all plate formats from 6- up to 1536-well plate reading.

Temperature-regulated heating plates above and below the entire plate movement area provide uniform incubation up to 45°C (optional 60`C).

Kinetic applications can he defined, with interval times from 20 ms to several hours. The fast filter wheels and the ability to define up to 8 sets of filters, each with individual gaintiming parameters, allow analysis of multiple fluorophores in a single well (FRET, dual-emission, DNA-detection.

Specifications

Light source                                        High energy xenon flash lamp

Detector                                              Side window, current type photomultiplier tube

Spectral range (nm)                           Ex: 355, 540-12 485, 584 Em : 460, 580-12, 520, 612

Gain control                                        Software selectable gain 1:4000 in 128 steps‑

automatic gain adjustment

Incubation                                           From ambient +5°C to 45°C in 0.1°C steps

Fluorescence Intensity                        Dynamic range 8 decades. Sensitivity 2 fmol /well

Fluorescein

Time-resolved fluorescence               Dynamic range 7 decades. Sensitivity 50 amol /well

Europium

Microplate formats                              Up to 1536 wells