manufacturer :       Biotage

model :                   PSQ 96MA

annotation :           Dokumente engl.

Meets the diverse genetic determination needs of clinical research laboratories :

•            Assays successfully developed for any genetic marker

•            Built-in quality control (QC) confirms genotyping results

•            Quick, simple and convenient to use

PSQTM96MA System is a multi-application genetic analysis solution designed to meet the diverse needs of clinical research. The versatile system combines SNP and mutation analysis with quantification capabilities and the sequenc­ing of short stretches of DNA to provide a complete genetic research solution.

The system uses proven Pyrosequencing technology to enable real-time, accurate genetic determination.

PSQ 96MA System comes with dedicated software. The complete platform also includes the Vacuum Prep Work­station for post-PCR sample preparation, optimized reagent kits and the Pyrosequencing Assay Design Service and Software.

Benefits of using Pyrosequencing and PSQ 96MA System

Assays successfully developed for any genetic marker

•            An unbeatable track record : a functional assay for any genetic marker (insertion, deletion, mutation, SNP or multiple polymorphism).

•            Simple and flexible assay design with minimal limita­tions in primer placement.

•            Multiple polymorphisms in close proximity are easily analyzed.

•            Suitable for rapidly mutating organisms – new mutations can be detected.

•            Quantitative data expands investigation options.

Built-in quality control (QC) confirms genotyping results

•            Pyrosequencing results (a PyrogramTM) present the sequence context surrounding the genetic variation.

•            Every sample contains its own confirmation of validity. No need for external controls.

•            QC based on the gold standard : DNA sequencing – the best confirmation there is.

Quick, simple and convenient to use

•            Easy to manage system within a multi-user environment.

•            Short learning curve – the PSQ 96MA System is up and running in one day with minimal training.

•            Convenient 96-well format.

•            Real-time results.

Versatile system

•            One complete solution to perform many different kinds of analyses – see table 1 for examples.

•            Flexible run setup – analyze one assay with up to 96 individual samples or up to 96 different assays in one plate

Versatility of the PSQ 96MA System

PSQ 96 MA System capabilities                       Clinical research application examples

SNP and mutation analysis                                 SNP association studies

                                                                            SNP and mutation genotyping

                                                                            Sub-tubing of microbes

Quantification                                                       CpG Methylation analysis

                                                                            SNP pooling studies

                                                                            Allele frequency quantification

                                                                            Gene copy number

                                                                            Loss of heterozygosity

Sequence analysis of short stretches of DNA    Bacterial identification

                                                                            Fungal identification

                                                                            Antibiotic resistance analysis

                                                                            Sequence siganture

Technical overview

The PSQ 96MA System consists of an automated instrument, with dedicated software for system control and data analysis, as well as optimized reagent kits, consumables and sample preparation tools. The system analyzes 96 samples simulta­neously, in a 96-well plate format, using the robust and reliable chemistry of Pyrosequencing technology. This eliminates the need for gels, dyes or specific labels.

Pyrosequencing technology uses an enzyme-cascade system, consisting of four enzymes and specific substrates, to produce light whenever a nucleotide forms a base pair with the complementary base in a DNA template strand. This light signal is detected, the base registered and the next nucle­otide added. If the added nucleotide is not complementary to the next base in the template, no light will be generated.

The PSQ 96MA System detects and transforms the light into signals, which are displayed as peaks in the resulting Pyrogram. The height of the signal is proportional to the number of bases incorporated, resulting in quantitative data.

Technical data and requirements

Input voltages                               110-120, 220-240 V

Power consumption                     Maximum 280 VA

Ambient temperature range         18-28°C

Ambient humidity                         30-90% relative humidity

Dimensions (HxWxD)                  490 x 520 x 650 mm

Clearance space (HxWxD)         800 x 520 x 700 mm

Weight                                          52 kg

Instrument temperature range     From room temperature up to 50°C

Instrument temperature               accuracy ±1°C

Process time, 96 samples          SNP analysis: ≥10 minutes

                                                    (9 dispensations/well)

                                                    Sequence analysis: ≥35 minutes

                                                    (30 dispensations/well)

Minimum computer requirements for an office installation.

Operating system                        WindowsTM 2000 or XP, English version

Processor                                    Intel Pentium III, 450 MHz

RAM                                             256 MB

Free hard disk space                   20 GB

CD-ROM                                      4x

Graphics card                              Super VGA

Monitor                                         SVGA 1152 x 864 pixels, 256 colors

Pointer device                              Mouse or similar