Firma:              Becton Dickinson

Modell:            FACS Vantage

Kommentar:   Dokumente engl. (für IDNR. 10644)

The FACS Vantage ^TM , BDIS's new cell sorter, was designed for ease of operation and provides ample room in which to work. With its stream-viewing video camera and monitor, an eight-trace oscilloscope, a wide selection of lasers, an optional third laser, FACS Vantage uses the latest technology in flow cytometry.

You can use FACS Vantage for research applications in:

Molecular Genetics to study

• isolated chromosomes for developing chromosome-specific libraries

• normal gene expression

• abnormalities leading to disease

Cell Cycle Analysis to

•     quantify the proliferative characteristics of cells and cell populations

•     gain knowledge about the fraction of cells at risk from cell-cycle—specific cytotoxic agents, enabling maximal tumor killing and minimal normal cell death

•     quantify phase fractions, phase durations, doubling times, labeling index, and growth fractions by using modeln or bromodeoxyuridine as a marker for cell­cycle traverse studies

•     employ the monoclonal antibody Ki-67, a nuclear antigen that is found in proliferating cells, but not in resting cells

•     perform leukemia phenotyping, monitor cancer remission, and analyze rare events in cancer studies

Intracellular Calcium Measurement to

•     measure intracellular ionized calcium concentration to learn about a cellular functions

•     correlate calcium concentration with other parameters, such as cell-surface antigen expression

•     sort, based an calcium concentration

Fluorescence In Situ Hybridization (FISH) to

•     label, locate, and quantify specific nucleic acid sequences in cells or chromosomes, alter sorting specific cells an FACS Vantage

•     enhance FISH techniques by adding the FACS Vantage spatial resolution, convenience, speed, and sensitivity

Large Particle Analysis and Sorting (using the available MacroSORT Option) to

•     characterize and sort islets of Langerhans for diabetes research including transplantation studies and encapsulation

•     measure nuclear genome sizes in higher plants by producing a suspension of cells without walls (protoplasts) and by measuring the DNA content

•     evaluate DNA content by studying megakaryocytes

Microbiology to

•     analyze changes in cellular functions brought about by intracellular organisms such as viruses, bacteria, and protozoa

•     study cell-cycle kinetics and regulatory mechanisms governing cell growth and replication

•     monitor (bacteria and other) microorganisms used in industrial processes or occurring as contaminants in materials ranging from drinking water to crude oil.

Enterprise II Performance Specifications

Utility and Environmental Requirements

Input Power                                       Single-phase with ground, 50 or 60 Hz

                                                         208-240 vac (±10%), 7.0 KVA maximum

Maximum Input Current vs.

Input Voltage                                     34 amps (rms) @ 208 volts (rms)

                                                         31 amps (rms) @ 230 volts (rms)

                                                         29 amps (rms) @ 240 volts (rms)

Cooling Water Flow Rate                 8 liters per minute (2 gpm) minimum

                                                         16 liters per minute (4 gpm) maximum

Incoming Water Pressure                1.4-4.2 Kg/cm ² (20-60 psi)

Incoming Water Temperature          10-45°C (50-110°F) at 8 liters/minute (2 gpm)

Heat Load to Cooling Water             4.5 kW (256 BTU/minute) maximum

Heat Load to Air at Power Supply     300 watts maximum

Ambient Air Temperature                 40°C (100°F) maximum @ Mean Sea Level (MSL)   (Derate @ 0.6° C per 1000 feet altitude when using the             LaserPure ^TM 5)

Operating Altitude                             3000 meters (9600 feet) maximum

System Weights                               Crated

Laser Head                                  53 kg (117 Ibs)

Power Supply                               52 kg (115 Ibs)

Uncrated

Laser Head                                  32 kg (70 Ibs)

Power Supply                               39 kg (87 Ibs)

Power Specifications

Model                         610       611       621 ²     622 ^2,3 651     652      653       DiscMasterN      DiscMaster Bioluminator _VS

Wavelengths (nm)

647.1                                                                                                                                                                       10

568.2                                                                                                                                                                       10

520.8                                                                                                                                                                       10

515 to 476                1000

514.5                         350                                100

488.0                         200                   180      100                                                                                                  10

457.9                                      80

413.1                                                                                                                        40

364 to 351                                          50         50         50      100 ⁴     80

350.7                                                                                                                                                 40

Beam Characteristics

Beam Diameter ^5,6              0.80 to 1.25 mm

Beam Divergence ⁵             0.60 to 0.80 mrad

Waist Diameters                0.70 to 1.00 mm

Waist Location ^5,7                0.80 to 1.20 m

Power Stability'                   <1% long-term (primary wavelength)

Beam-Pointing Stability ⁸     <5 mrad/°C typical

Optical Noise ^9,10                 <1% rms (0.75% rms/6% peak-to-peak typical)

Mode/Mode Purity ¹¹            TEM ₀₀ / M ² ≤1.20

Polarization Ratio               100:1

1        All performance specifications are measured at the powers and wavelengths listed. Powers are listed in milliwatts.

2        Multiline UV is the primary output used for light regulation. At the specified UV power, secondary wavelength output will be much higher than specified values.

3        The output also contains other wavelengths whose power levels are unspecified

4        Unpolarized.

5        Typical values. Specific values will vary for individual wavelengths and from model to model.

6        Beam diameter is measured at the output coupler.

7        Beam waist is measured from the output end of the laser head enclosure.

8        Maximum peak variation over any 30-minute period after a 30-minute warm-up.

9        Measured with a 10 Hz to 2 MHz photodiode driving a resistive Ioad.

10      Bioluminator35Models at 568.2 nm and 647.1 nm display <_ 3% rms.

11      In multiple wavelength outputs, the M ² specification applies separately to each wavelength.