Firma:              Ciphergen

Modell:            PBS II

Kommentar:   Dokumente engl.

The ProteinChip System

Ciphergen's ProteinChip System is comprised of a ProteinChip Reader (series PBS II) integrated with ProteinChip Software and a personal computer to analyze proteins captured on ProteinChip Arrays. The ProteinChip System detects and accurately calculates the mass of compounds ranging from small molecules and peptides of less than 1000 Da up to proteins of 500 kDa or more based on measured time-of-flight. The System is compact enough to fit into almost any lab space, allowing researchers direct access to precision mass analysis of important peptides and proteins from complex biological samples.

ProteinChip Arrays

Ciphergen's ProteinChip Arrays distinguish this technology from other mass spectrometry-based analytical Systems. ProteinChip Arrays provide a variety of surface chemistries that allow researchers to optimize protein capture and analysis. The surface chemistries of the arrays include a series of classic chromatographic chemistries and specialized affinity capture surfaces. Classic chromatographic surfaces include normal phase for generic protein binding; hydro­phobic surfaces for reversed-phase capture; cation- and anion-exchange surfaces; and immobilized metal affinity capture (IMAC) for metal-binding proteins. Specific proteins of interest can be covalently immobilized on pre-activated surface arrays, enabling customized experiments to investigate antibody-antigen, DNA-protein, receptor-ligand, and other molecular interactions.

The ProteinChip Reader (Series PBS II)

The ProteinChip Reader is a laser desorption/ionization time-of-flight mass spectrometer that uses state-of-the-art ion optic and laser optic technology. The laser optics maximize ion extraction efficiency over the greatest possible sample area, and thus increase analytical sensitivity and reproducibility. The Reader's ion optics incorporate a four-stage, time-lag-focusing ion lens assembly that provides precise, accurate molecular weight determination with excellent mass sensitivity.

The ProteinChip Software

Ciphergen's ProteinChip Software controls all aspects of the ProteinChip Reader and facilitates data collection and analysis. The Software uses a Microsoft Windows NT interface, and contains numerous features, including automatic reading of ProteinChip Arrays; multiple spectrum comparison for differential protein display and biomarker discovery; several alternative viewing formats for data; and a user-friendly interface.

Technical Specifications

The ProteinChip Reader is a linear laser desorption/ionization time of flight mass spectrometer.

Enclosure

• Floor-standing unit with castors.

• Dimensions: 98 cm (38.5") wide x 56 cm (22") deep x 71 cm (28") high.

• Mass: 145 kg (320 pounds).

Mass Analysis Range

• From less than 0.1 kDa to greater than 500 kDa.

Sensitivity

• Better than 300:1 average signal to noise ratio on the analysis of 50 femtomole of bovine IgG when digitally smoothed with a (0.2 times peak width) sliding window average.

Mass Resolving Power

Automatic Mode

• Better than 600 average resolution for 10 "spot protocol" auto­mated 25-shot averages of 5 picomole of human recombinant insulin.

Manual Mode

• Isotopic envelope limited resolution of better than 1000 for the analysis of 5 picomole of human recombinant insulin.

Mass Accuracy

Internat Calibration, Absolute

• Better than 0.02% absolute error for eight measurements of 1 picomole of bovine insulin B chain calibrated against soma­tostatin and human recombinant insulin on an 8-position, "A-H," normal phase ProteinChip.

Internat Calibration, Averaged ¹

• Better than ± 0.5 Da 0.5 to 5 kDa

• Better than ± 200 ppm (0.02%) 5 kDa to 10 kDa

• Better than ± 500 ppm, (0.05%) 10 kDa to 300 kDa

External Calibration, Absolute

• Better than 0.1% average absolute error for seven analyses of human angiotensin I, somatostatin, bovine insulin B chain, and recombinant human insulin (~1 picomole each) as analyzed on an 8-position, "A-H," normal phase ProteinChip in which spot D is used as a calibration run.

External Calibration, Averaged ²

• Better than ± 1 Da 0.5 to 1 kDa

• Better than ± 1000 ppm (0.1%) 1 kDa to 10 kDa

• Better than ± 2000 ppm (0.2%) 10 kDa to 300 kDa

Ion Source

• Laser desorption ionization

• Radial-focusing electrostatic optics

• Operating voltage: 0- ±20 kV (standard software selectable settings: +20 kV, +6 kV, -6 kV)

• Factory burn-in: stable to ±30 kV at termination of conditioning procedure

Flight Tube

• 0.8 m

Ion Detector

• Minimum peak width <8 ns

• Linear dynamic range: linear output to 10 ⁹ electrons (500 mV for a 10 ns pulse)

• Large-pulse recovery <30 ns

• Noise <2 mV rms 0-125 MHz

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Transient Digitizer

• Trigger jitter: < 2 ns

• Aperture jitter: <10 ps

• Sampling rate: 250 MS/s or 500 MS/s

• Record length: >2 ms

• Input amplifiers: multi-gain linear and logarithmic

Dynamic Range

• 8 bits (256 signal intensity levels per scan on board; 32 bit signal averaging on computer)

• Linear dynamic range software controlled in 9 steps (3-4 dB/ step) from -0.020 V to -1 V full-scale

• Logarithmic dynamic range, 0 to 1 V

High Voltage Pulser

• Trigger jitter: <1 ns

• Lag time: 0.17-5 seconds

• Pulse height: 0-5 kV

• Polarity: selectable under computer control

• Risetime: 100 V/ns

• Pulse width: 0.1-25_seconds

Laser

• Pulsed nitrogen laser (337 nm) 4 ns pulse width

• Intensity adjustment: 300 steps full-scaled 0-150 mJ logarithmic.

• Spot size: 200 mm x 1000 mm

• Maximum pulse rate 10 Hz

Sample Handling

• Linear sample arrays on 9 mm x 78.5 mm x 2.4 mm (72 mm accessible for analysis)

• Vacuum load-lock for rapid sample introduction (<3 minutes from insertion to data acquisition)

Vacuum System

• Base pressure <2 x 10 ^-7 torr (no baking).

• Pressure less than 1 x 10 ^-6 immediately after sample exchange.

• 250 L/second high compression turbomolecular pump with two-stage rotary-vane backing pump.

• Gauges: active inverted magnetron (high vacuum) and active pirani (low vacuum)

Control System

• Windows NT-based software for automated instrument opera­tion and data analysis

• High end personal computer and monitor

• GPIB interface with >750 MB/s peak data transfer rate

• Data rate: 1-4 spectra per second; Analysis of one "A-H" Pro­teinChip Array with eight 50-spectrum averages takes approxi­mately 10 minutes.

Supply Voltage

• 110-120 VAC, 10A or 220-240 VAC, 5A 50-60 Hz; 100 VAC available as option.