manufacturer :       BioRad

model :                   Protean II xi

annotation :           Dokumente engl.

Introduction

The PROTEAN II xi cell is a vertical slab electrophoresis instrument which combines versatility with practicality. When used with the various combs, spacers, and accessories avail­able, the PROTEAN II xi cell is suitable for most common electrophoretic techniques, including SDS electrophoresis, two-dimensional (2-D) electrophoresis, native electrophoresis, and agarose gel electrophoresis. The PROTEAN II xi cell can run up to 4 slab gels or 16 tube gels simultaneously. The basic unit accommodates gels 16 or 20 cm long. The 20 cm gels offer increased resolution capability, which is especially useful in 2-D applications.

The central cooling core of the PROTEAN II xi cell assures even heat distribution over the entire gel length, permitting excellent resolution with minimal band distortion. Only 1.5 liters of buffer are required. The raised electrode position insures safe operation even with extended overnight runs.

The unique single-screw sandwich clamps allow rapid assembly of the gel sandwich­es, while providing even pressure distribution along the entire gel length. This even pres­sure minimizes the risk of breaking the glass plates, a common problem with multi-screw clamps. The PROTEAN II xi alignment card helps keep spacers upright during sandwich alignment. The combination of the clamps, alignment card, and the casting stand permits assembly and casting of gels in minutes, with little effort. After casting, the completed gel sandwich attaches to the central cooling core with a single motion.

The PROTEAN II xi cell is the instrument of choice for 2-D electrophoresis. With the optional tube adaptors, one can run the first dimension IEF tube gel, and then overlay this gel onto the second dimension SDS slab gel. Thus, the complete 2-D procedure can be done with one dedicated instrument.

Specifications

Construction:

  Cooling core and

  tube gel adaptor                         molded polysulfone

  Lid and lower buffer

  chamber                                     molded polycarbonate

  Clamps, casting                         glass and Teflon®-filled molded poly‑

  stand , and cams                        carbonate

Electrical leads                            flexible, coiled

Electrodes                                    platinum, 0.010 inch diameter

                                                    (0.254 mm)

Shipping weight                            11 kg (24 lb, 3 oz)

Overall size                                  26 cm (L) x 19 cm (W) x 30 cm (H)

Gel size                                        16 x 16 cm slab or 16 x 20 cm slab

                                                    1 to 6 mm diameter tube gels

Glass plate sizes                         16 cm cell: 16 x 20 cm (inner plate)

                                                                      18.3 x 20 cm (outer plate)

Cooling core, maximum

flow rate                                       2 liter/min

Maximum coolant temperature    Do not exceed 50 °C

Voltage limit                                  1,000 volts DC