Firma:                  Knauer

Modell :                AminoNova A200

Kommentar:       Dokumente engl.

Specifications :

Elution Buffer System

Eluent carousel for eight 500 ml non-toxic, ready-made buffers:

• Five lithium acetate buffers ( Eluents A, B, C, D, and E)

• Column regenerant , lithium hydroxide ( Eluent F)

• Potassium acetate ninhydrin reagent

• Reactor wash solution

One eluent system for both hydrolysis and physiological analyses! Ninhydrin reagent and eluents have 1 year shelf-life

Automated Sample Injection System

Integrated XYZ-mechanism with stationary rack design 2 sample racks accommodate 120 standard, 1.5 ml vials Injection volume in 1 μ l increments from 1–40 μ l Sample cooling to 5°C ± 2°C; Five priority sample positions

Detectors

2 microphotometer detectors at 570 nm and 440 nm Measurement cell with 10 μ l volume, 10 mm pathlength

Measurement range in 2 sensitivity options, normal and high (10x) Analog output 0-4 V; Noise < 0.02 mAU ;

Drift < 0.01 mAU /hr Linearity error < 0.3% at 1 AU; < 2% up to 4 AU

Peltier Column Oven

New design provides for longer lifetime than A100 column oven Programmable from 20–80°C; accurate to 0.3°C Rapid temperature ramping up to 5°C/min

Postcolumn Reactor

PEEK 0.25 mm ID reactor coil inside reactor oven unit Programmable from 20–150°C; accurate to 0.5°C

Pump for delivery of eluent and ninhydrin reagent

Double piston pump with 10 ml pumphead , inert titanium inlays Flow rate 0.01–1.50 ml/min

(typical flow rate = 0.20 ml/min) Reproducibility of flow is better than 1% at 100 μ l/min Pulse dampener reduces baseline noise to < 0.3 mV/min (570 nm)

Instrument Control and Data Management

Total instrument control from 32-bit aminoControl software under Microsoft Windows 2000 environment

Data acquisition with ChromGate or EuroChrom software GLP features and 21CFR11 compliant (log file, data audit trail, etc) Analog signal output and RS-232 connector

Reproducibility, detection limit

Retention times ≤ 2% RSD; peak areas ≤ 5% RSD 15 pmol detection limit (sensitivity varies for diff. amino acids)

The Amino Acid Analyzer A200 is a dedicated, compact, and complete analysis system, developed exclusively

for the routine analysis of amino acids by post column derivatization with ninhydrin . It represents a significant advancement in facilitating the determination of amino acids:

• The buffer system and separation column are compatible for both hydrolysis and physiological analyses

• Innovative narrow-bore design results in shorter run times and less eluent and ninhydrin reagent consumption

• Sensitivity is approximately four times greater than the previous generation of postcolumn ninhydrin analyzers

• All components are easily exchanged for faster service turnaround time

• Delivers outstanding results at an exceptional price

The widely known and extensively used ion exchange postcolumn ninhydrin procedure allows detection of both primary and secondary amino acids with the highest resolution and accuracy. Sample preparation is kept to a minimum and the step-wise elution permits an optimal separation while assuring excellent reproducibility.

Complete instrument control is achieved through aminoControl software running under Microsoft Windows 2000. The aminoControl software’s user-friendly interface and graphical displays of program parameters make routine analysis with the A200 simple and fast. A status information screen is always visible and gives an instant report on the current instrument operation. Five prewritten analysis programs and space for 49 additional custom programs makes it easy to optimize the running parameters for complicated analyses.

• Three password-protected access levels provide for GLP compliance

• An automatically generated log file maintains a record of all user actions

Clinical, research, or routine analysis

The A200 was developed primarily for the routine analysis of feedstuffs, agricultural products, or quality control (pharmaceuticals, food products, etc.). Clinical and biomedical laboratories can also use the A200 with physiological samples to detect and diagnose metabolic diseases or to quantitate amino sugars, peptides, or any other ninhydrin -reactive compounds. Applications are available upon request.