manufacturer :       Applied Biosystems

model :                   API 2000

annotation :           Dokumente engl .

The API 2000TM LC/MS/MS System:

Triple Quadrupole Performance in a Compact Benchtop Footprint Featuring

Applied Biosystems /M DS SCI EX Ion Sources for Maximum Flexibility and Ease-of-Use

Overview

The API 2000TM LC/MS/MS System brings all of the power and performance of triple quadrupole mass spectrometry right to your benchtop . Other instruments claim to have a benchtop design - but use the entire bench. The API 2000 System leaves plenty of room for everything else in your lab. Its footprint measures a compact 45 cm X 100 cm.

In addition to the small footprint, the API 2000 LC/MS/MS System features robust ion sources from Applied Biosystems /MDS SCIEX. These sources offer full articulation with large viewing ports for easy optimization. To prevent contamination during switching, the sources

are positively vented and include the patented Curtain Gas ^TM interface to maximize ion transfer to the mass analyzer and provide superior ruggedness and reliability. Since safety is always a concern, the ion sources are fully insulated so that operators are protected from hot surfaces. Because time is so valuable, the ion sources for the API 2000 System are truly “plug and play,” thus allowing for a quick changeover between the sources to keep operations running smoothly without sacrificing productivity.

In general, triple quadrupole mass spectrometers are very popular analytical instruments. They offer many key benefits to researchers who are interested in high throughput analyte quantitation using a mass detector of high specificity coupled to a liquid chromatograph. Triple quadrupole MS/MS is one of the most sensitive and specific technologies available for small molecule quantitation . This technique is amenable to a broad range of compounds and inherently eliminates the need for extensive sample preparation. The fragmentation capabilities of tandem mass spectrometry also provide critical information for structural elucidation of unknown compounds.

Some typical applications for triple quadrupoles include:

• Drug Metabolism and Pharmacokinetics (DMPK)
• Environmental analysis
• Forensic toxicology
• Medical research
• Drug delivery
• Protein and peptide characterization
• Impurity and metabolite profiling

Key Features of the API 2000 LC/MS/MS System

• Small footprint
• LINACTM collision cell for high throughput Multiple Reaction Monitoring (MRM)
• Automated methods development
• Built-in syringe pump
• Economical
• Backed by a worldwide service and support network

Proven Performance

You won’t have to sacrifice performance for size. The API 2000TM System’s proven engineering includes the proven Curtain Gas ^TM interface and the LINAC ^TM collision cell.

The latter allows for improved scan speeds and increased sensitivity while preventing contamination and eliminating cross-talk.

The Curtain Gas interface employs a nitrogen stream flowing countercurrent to the ion path. This gas flow drives solvents and buffers from the inlet, maximizing ion transfer to the analyzer, thereby providing unmatched ruggedness and reliability. Efficient Ionization is achieved without heat. This eliminates the possibility of thermal degradation of labile compounds.

The “Gold Standard” in Ionization Performance

Applied Biosystems /MDS SCIEX offers two ion sources for the API 2000 System to accommodate all of your analytical demands:

Heated Nebulizer or Atmospheric Pressure Chemical Ionization (APCI) Source

• Full gradient capabilities
• Computer controlled probe temperature and gas flow
• Optional Supercritical-Fluid Chromatography (SFC) compatibility
• Flow rates up to 2 mL per minute
• Allows ionization of neutral molecules
• Species are less susceptible to ionization suppression
• Wide dynamic range
• LC “dead” volume is less of a consideration
• Higher buffer concentrations are acceptable

TurboIonSpray ^TM Source

• Permits flow rates from 2 µL to 1 mL per minute
• Exhibits excellent concentration dependent response
• Ability to analyze high molecular weight compounds
• Very sensitive
• Wide range of accepted analytes
• Experiences none of the problems associated with thermal lability or volatility

Ease-of-Use

Never before has an LC/MS/MS system been so user friendly. The APCI and TurboIonSpray sources can be completely inter­changed within minutes without tools. The following three steps illustrate the ease with which the sources can be switched

.

All temperature, gas and electrical connections are fully integrated into the source housing so there are no extra lines to attach. This seamless conversion is completed when magnetic connections automatically detect and indicate the hardware change to the software. There is no need to “tell” the software what source you are using. The built-in syringe pump and diverter valve simplify hardware configuration and also contribute to conserving bench space.

Superior engineering allows for reliable flow rates as low as 2 µL per minute and up to 2 mL per minute. In addition, ion sources are safety interlocked - voltages are automatically set to zero when either source is removed.

All mass spectrometers require occasional cleaning and maintenance. Maintenance is simple with the API 2000TM System. The system covers are easily removed with common tools, and components such as the curtain plate, orifice, skimmer, and entrance quadrupole are readily accessible for cleaning.

The API 2000 System also features Analyst ^TM software for acquisition, instrument control and data processing. Analyst software simplifies data reduction in the familiar Windows NT® operating environment. Automatic tuning and calibration ensure optimum performance while rapid data acquisition increases your productivity, allowing you to spend valuable time exploring and interpreting data instead of on system set-up. Analyst software further streamlines your system by offering single point control over a wide selection of LC pumps, autosamplers and liquid handling systems.

Conclusions

Laboratories of all sizes can benefit from the advantages of tandem mass spectrometry with the API 2000 LC/MS/MS System. This rugged system gives you the high throughput and specificity that you need in an economical, benchtop platform. Productivity is improved even further with the API 2000 System because of its rugged and easy to use ion sources. The Curtain GasTM interface and LINACTM collision cell are instrumental in providing superior

performance . Experience the benefits of a triple quadrupole instrument with a smaller footprint and less maintenance than you would expect.

Optional Equipment:

Perkin Elmer Series 200 HPLC Systems

Series 200 Autosampler

The PerkinElmer Series 200 LC Autosampler is an advanced sample processor that can be used with virtually every liquid chromatography system or application. You configure the Autosampler and define all of your methods and operations using function keys on a key­board located on the front panel of the instrument. A backlit Liquid Crystal Display (LCD) located directly above the keyboard shows both status information about the instrument and provides access to the screens you use to define methods and set configuration parameters. If you use the PerkinElmer Series 200 LC Pump, you will find that the Autosampler front panel is almost identical to the one on the Pump. In addition, the Pump and the Autosam­pler share many common operating features that will allow you to get your system up and running quickly. The Series 200 LC Autosampler provides you with interchangeable technol­ogy so that you do not have to buy a new Autosampler and learn new user interfaces every time your needs change.

  Features and Benefits

Ø       Upgradable to biocompatible versions for maximum flexibility.

Ø       Small, compact design (12.5 inches (31.8 cm) wide) saves valuable bench space.

Ø       Directory of up to 20 methods.

Ø       Ability to link up to 10 methods together in a sequence.

Ø       Priority method which allows you to interrupt a method or sequence to run critical sam­ples. The method or sequence resumes once the priority samples are analyzed.

Ø       Edit the active method or sequence in real-time with easy access function keys.

Ø       Large backlit liquid crystal display allows clear viewing of autosampler method and operating parameters at all times.

Ø       Tactile keyboard with unique “key click” so that you know your entry has been made.

Ø       Phone type contact closure connections and finger tight plumbing fittings make it simple to connect or disconnect your system.

Ø       Full method documentation including modification with method name, number, date and time stamp.

Ø       Visual access front-door panel allows quick plumbing inspection and easy servicing.

Ø       Maintenance log coded by the serial number of the autosampler , including autosampler injection cycles.

Ø       Instrument log records history of operation for quick diagnosis and servicing to get you up and running quickly.

Ø       Extensive service diagnostics .

Ø       Sampler module can be completely removed to lab bench for maintenance without having to unstack modules.

Specification Series 200 Autosampler

Injection range                            Programmable from 0.1 - 1.0 µL at 0.1 µL increments, 1 µL to 2.5 mL at

                                                  1 µL increments

Replicate injections                    1 - 99 from one vial

Precision                                    Typically <0.5% RSD of peak areas from 3 µL

Linerarity                                    >0.999

Minimum sample volume           1 µL in 7 µL

Sample syringe sizes                50, 100, 250, 500, 1000, 2500 µL

Sample waste                            None (or user selectable)

Carryover                                   <0.02%

Flush cycles                               User selectable, 1 - 9

Flush volume                             0 - 2500 µL

Sample capacity                        up to 225 vials with optional tray

Method parameters                    Injection volume, sample draw speed, flush volume, flush cycle,

                                                  2 external contacts, start and stop time

Methods                                     Battery-backed storage of up to 20 methods including time pro‑

                                                  gramming . Editing of stored methods possible during runs.

Injection cycle time                    2 - 3 samples per minute

Valve materials in contact with With Rheodyne 7725 for Analytical - stainless steel, Vespel , sample       ceramic, PEEK

                                                  With Rheodyne 9725 for Bio Applications - PEEK, Tefzel , ceramic

                           With Rheodyne 8125 for Microbore - stainless steel, Vespel , ceramic, ^             PEEK

Display                                       7-line by 40-character backlit liquid crystal display

Communications                       Integrated communications for derivatization and dilution option s

                                                  Serial communications - standard

                                                  BCD interface - standard

                                                  5 relay outputs; 2 timed events, 2 inject, 1 start

AC Power Requirements:

AC power                                   70 Watts

AC voltage range                       Grounded 100 ,120,220,230,VAC (±10%)

                                                  Grounded 240 VAC (+6%/-10%)

Frequency                                  50/60 Hz (±1%)

Fuse rating characteristics:

100/120 VAC Single Fuse          1.6 A 3 AG Slow Blow, 250 V

220-240 VAC Single/

Dual Fuse                                  0.8 A TT (5x20mm), 250 V

Operating Conditions:

Environment                               This instrument is desigend for indoor use. It is not designed to operate in an explosive environment.

Ambient temperature                 10 -30 °C

Ambient relative humidity           10 -80% (without condensation)

Altitude                                       0 to 2000 meters

Installation Category                  This instrument is able to withstand transient overvoltage according to Installation Categroy II as defined in IEC 1010-1.

Pollution Degree                        This instrument will operate safely in environments that contain nonconductive foreign matter to Pollution Degree 2 in IEC 1010-1.

Peltier sample cooling

temperature range                     4 – 50°C, ± 2°C

Series 200 Mixer

Specifications

Specification                                                               Description

---------------------------------------------------------------------------------------------------------------------------

Power Requirentents :

Maximum Power                                                        20 VA

Line Voltage Operating Range                                   100/120 or 220/230/240 VAC ±10%

Frequency                                                                  50/60 Hz

Line Cord                                                                    See "The AC Line Cord"

Fuse Rating Characteristics:

120 VAC (P/N 0999-1643)                                         0.1 Amp, Type T, 250 Volt

240 VAC (P/N 0999-1643)                                         0.1 Amp, Type T, 250 Volt

Operating Conditions:

Ambient Temperature                                                +5 to 40°C (40 to 104°F)

Ambient Relative Humidity (RH)                                 20 to 80% RH (without condensation)

Altitude                                                                        0 to 2000 m

Storage Conditions:

Ambient Temperature                                                -20 to +60°C (-4 to +140°F)

Ambient Relative Humidity                                         20 to 80% RH (without condensation)

Altitude                                                                        0 to 12,000 m

Dimensions/Weight:                                                  Height: 11.5 cm (4.5 in.)

Width: 7.0 cm (2.8 in.)

Depth with Mixer Head: 29 cm (11.4 in.) Weight. 1.5 kg (3.3 lb.)

Series 200 Vacuum Degasser

About the Series 200 Vacuum Degasser

The PerkinElmer Series 200 Vacuum Degasser is a high- efficiency, online module that removes dissolved gasses from HPLC solvents. Its unique design assures reliable continuous operation and the highest level of continuous performance available without the need for helium degassing. The five-channel design is compatible with isocratic, binary, and quaternary pumping systems, and includes an additional channel to provide a separate degassed solvent to the autosampler needle wash port. A three-channel design is also available and is compatible with binary pumping systems that include an autosampler . The figures shown in this manual are for the quaternary version of the degasser. The multi-pass design, (five channels) allows increased flexibility in that you can connect one quaternary pump and an autosampler , two binary pumps and an autosampler or four isocratic pumps and an autosampler . Also, this design permits the use of higher flow rates by passing the solvents through the chambers several times. The extremely low internal volume of each Teflon AF ^® channel (400 microliters ) offers very quick equilibration and very short startup times compared with PTFE degassing channels which have the same degassing efficiency.

Inside the unit, the solvent flows through a short length of Teflon Ar tubing which has a volume of 400 microliters in a vacuum chamber. Within this chamber a partial vacuum is maintained, i.e. a pressure below 54 mm of Mercury (Hg) absolute by a constantly running, low RPM vacuum pump. Dissolved gasses are pulled through the tubing wall by the vacuum as the solvent flows within the coil, in accordance with Henry's Law. Gasses removed are expelled, and the chamber is maintained at the maximum vacuum level attainable by the vacuum pump.

A special port in the vacuum pump continually flushes the pump head with a small "bleed" of air to remove any solvent vapors that may enter the pump from the vacuum chamber. This air bleed eliminates the need for any solenoid valves within the system. This design results in zero vacuum " hysteresis ". Previous designs allowed the vacuum chamber pressure to fluctuate, with the pump cycling an and then off in response to the vacuum level. A patent is pending an this design concept.

Series 200 LC Pump

The PerkinElmer Series 200 LC Pump can be used with virtually every LC system or application. The Series 200 LC Pump is upgradable from a single solvent (isocratic) pump, to mixing two solvents (binary) or to mixing four solvents (quaternary). In addition, each version is available in a biocompatable version. The Series 200 LC Pump provides you with the interchangeable technology so that you do not have to buy a new pump and learn new user interfaces every time your needs change.

Series 200 LC Pump Features and Benefits

• Upgradable isocratic, binary, and quaternary versions as well as biocompatible versions for maximum flexibility.
• Small, compact design (12 inches (30.5 cm) wide) saves valuable bench space.
• Directory of up to 20 methods, 20 steps each with built-in method linking increases sam­ple throughput.
• Edit the active method or sequence in real-time with easy access function keys.
• Large backlit liquid crystal display allows clear viewing of pump method and operating parameters at all times.
• Unique function allows automatic shutdown of the pump method at final gradient method.
• Graphic display of solvent profile on-screen Shows real-time status of the gradient method.
• Hold gradients or advance to the next step with a simple keystroke.
• Solvent-resistant front panel with rounded ergonomic design for easier interaction with the keypad.
• Tactile keyboard with unique "key dick" so that you know your entry has been mode.
• Phone-type contact closure connections and finger tight plumbing fittings make it simple to connect or disconnect your system.
• Full method documentation including modification with method name, number, date and time stamp.
• Visual access front-door panel allows quick plumbing inspection and easy servicing.
• Maintenance log coded by the serial number of the pump, including pump and seal cycles.
• Instrument log records history of Operation for quick diagnosis and servicing to get you up .and running quickly.
• Extensive service diagnostics.
• Pump module can be completely removed to lab bench for maintenance without having to unstack modules.

Main Components of the Series 200 LC Pump