manufacturer:       Applied Biosystems

model:                   7500

annotation:           Dokumente engl.

What is the Applied Biosystems 7500 Real-Time PCR System?

The 7500 System is a thermal cycler that performs real-time PCR analysis. It is validated for use with the Quantifiler ^TM Human DNA and Quantifiler Y Human Male DNA quantitation kits to determine the amount of human and/or male DNA that is contained within a forensic and database sample.

What is Real-Time PCR?

Real-time PCR measures PCR amplification as it occurs, cycle-by-cycle, allowing quantitative measurements to be made in the highly reproducible exponential phase of PCR, enabling extremely accurate and precise quantitation of DNA over a large dynamic range. Real-time PCR provides the highest sensitivity and widest dynamic range of all available nucleic acid detection systems. Real-time PCR using TaqMan® technology is widely regarded as the gold standard for DNA & RNA quantitation due to its reliability and the increased specificity obtained through using primers and a probe.

Laboratories use the Applied Biosystems 7500 Real-Time PCR System in conjunction with the Quantifiler Human DNA and Quantifiler Y Human Male DNA quantitation kits to deterrmine the amount of amplifiable total male and total human DNA in a sample to estimate the relative amounts of male and female DNA in sexual assault forensic samples. Results obtained from the 7500 system using the Quantifiler kits also provide an indicator of the potential presence of PCR inhibitors and/or confirm the presence or absence of DNA in a sample. In addition, the Quantifiler kits determine the optimal amount of DNA that should be used in downstream STR amplification reactions to achieve a complete, balanced STR profile.