manufacturer :       Appl. Biosystems

model :                   StepOne

annotation :           Dokumente engl.

Applied Biosystems’ 48-well low throughput real-time PCR instrument, the StepOne™ Real-Time PCR System, makes it simple and easy to get publication ready high quality data on day one. This remarkably simple real-time PCR system is designed with a user-friendly yet powerful interface for researchers new to real-time PCR. Bring total confidence to your research with the StepOne™ System that collects and saves data from all filters in every run without depending on a computer or plate setup.

• Intuitive, flexible software and wizards guide new users through their real-time PCR experiments in three easy-to follow steps
• Data stored both on the instrument and computer for extra safety
• Long-life LED-based optical system records fluorescence from FAM™/SYBR® Green, VIC®/JOE™, and ROX™ dyes for gene expression analysis, pathogen quantitation, SNP genotyping, and presence/absence assays
• Fast system with plug and play assays and reagents for maximum time savings
• Ultra-compact footprint fits any laboratory setting
• LCD touchscreen and USB drive with the option of PC-free instrument operation
• Remote monitoring and email notification with run file attached for immediate access to results--even remotely
• Easily upgradeable to the StepOnePlus™ Real-Time PCR System

New StepOne Software v2.1
Instrument software for the Applied Biosystems StepOne Real-Time PCR System runs on the Windows XP® operating system and provides instrument control, data collection, and data analysis.

Now with the Powerful Gene Expression Study Package

• Expand your research by analyzing an unlimited number of plates in one study
• Take advantage of the flexibility to sort data by biological or technical replicate group
• Achieve greater accuracy with multiple endogenous controls
• Increase precision with assay efficiency correction in gene expression analysis

Software Highlights

• Experimental design wizards to help you design and set up experiments
• Advanced setup if you require more flexibility for complex applications such as multiplexing
• QuickStart setup so that you start a run immediately and enter plate information at a later time
• Real-time monitoring of amplification growth curves enables you to view run progress
• Remote real-time monitoring so that you can monitor progress from a remote PC with the option to download the run file
• Email notifications with data file attached for immediate access to your results--even remotely
• Multiple plots view so that you can simultaneously assess data from four different perspectives
• Export easily to PowerPoint, Excel, and graphical images

Remarkably Simple System
The StepOne™ system brings advanced real-time PCR technology to a new level of accessibility—even for first-time users. The system measures amplification as it occurs, cycle by cycle, allowing for precise and quantitative measurements during the exponential phase of PCR. Beginning at the StepOne™ system homepage, you can seamlessly navigate through all aspects of the real-time PCR method including sample and reaction set-up, thermal cycling, and fluorescent detection. The software is even pre-populated with the right run protocol for each application when using reagents from Applied Biosystems. Just enter your plate set up and go. Depending on the experimental design, the system can even help you select and order real-time PCR reagents online by means of convenient links in the Design Wizard. The StepOne™ System is a long life LED based optical system that records fluorescence from FAM/SYBR Green/VIC/JOE and ROX dyes for gene expression analysis, pathogen detection, SNP genotyping and presence/absence assays. This cost-effective, 3-color, 48-well system delivers precise, quantitative real-time PCR results in which you can have total confidence.

Simply Remarkable Results
Because the StepOne™ system is factory-calibrated for optical and thermal accuracy, simply remarkable real-time PCR results are available right out of the box. It can discriminate between 2 populations of 5,000 and 10,000 template copies of a TaqMan® assay with 99.7% confidence.

Applications
The StepOne™ Real-Time PCR System supports many applications including:

• Standard Curve (absolute quantitation)
• Relative Standard Curve
• Comparative Ct (DDCt) (relative quantitation)
• Genotyping and Presence/Absence
• Melt Curve Analysis (as a standalone application)

Upgrade your StepOne™ System to a StepOnePlus™ System
The StepOnePlus™ Real-Time PCR System Upgrade is an easy upgrade solution for your StepOne™ System. You can go from 3 colors and 48 wells to 4 colors and 96 wells in no time at all. You can even receive a StepOnePlus™ instrument on loan so you can continue your research during the upgrade process.

Specifications