Firma:              Appl.Biosystems

Modell:            BioCAD 700E

Kommentar:   Dokumente für IDNR. 10123

Overview

PerSeptive Biosystems BioCAD ^® 700E Workstation for Perfusion Chromatography ^® is a computer-aided chromatography workstation that integrates all aspects of biomolecule purification with Perfusion Chromatography technology.

The BioCAD 700E Workstation is designed for:

•     Chromatographic method execution —Routine execution of chromatographic methods for the purification and analysis of biomolecules.

•     Systematic method development —The goal of method development is to optimize conditions for your application. Systematic method development makes it easy to identify variables that affect your application, systematically change the variables, observe the effects, and use the information to optimize chromatographic conditions.

The BioCAD 700E Workstation provides the following features:

•     Simplicity —Microsoft ^® Windows ^® -based software that is intuitive and easy to use. You can efficiently develop and execute separations for analytical and preparative applications.

•     Full Process Capability —Full capability from sample injection through fraction collection, including automated column washing and regeneration.

•     Speed —Chromatographic separations in as little as one to three minutes, when using POROS ^® media and columns.

•     Versatility —Multiple column configurations and injection mode options.

•     Gradient Capability —Six-buffer/solvent capability, with simultaneous blending between any four buffers/solvents.

•     Detection —Monitoring of pH, conductivity, dual-wavelength UVNis absorbance, fluorescence (optional), and system pressure.

•     Templates —Predefined templates for systematic method development of key variables such as pH, gradient slope, and sample load.

•     Data Analysis —Data analysis of single data files or groups of data fites.

•     SCOUT ^® Column Selector —Automated multi-column switching device that allows flow to be diverted to any one of six attached columns (optional).

Parts of the system

The BioCAD 700E Workstation includes:

•     Front panel indicators and controls

•     Buffer/solvent and pump system

•     Sample Ioader

•     Columns and plumbing

•     Detector and monitors

•     Fraction collection

•     Computer components

Figure 1-2 BioCAD 700E Workstation

Specifications

Detection

Miscellaneous

Advantec Super Fraction Collector SF-2120

SPECIFICATIONS

FRACTION

Test tube                                               120 tubes (Ø12-Ø18 mm x 90-180 mm H)

Modes                                                    Simple, Peak, Window, Manual, Bottle

Dropper assembly movement time      Typically 0.1 sec. (center to center)

MODE, PARAMETER

Simple {Open Column} Mode

          Fraction mode      Time                 99 min. 59 sec./tube MAX.

          (Capacity)             Drop                 9999 drop/tube MAX.

                                      Signal               9999 count/tube MAX.

                                      Volume             999.9 mL/tube MAX.

Peak {Multisample} Mode

          End time                                       999 min. MAX.

          Fraction peaks                             10 peaks MAX.

          Fraction capacity                         99 min. 59 sec./tube MAX.

          (Request & Monitor)

Window {Multisample + Peak} Mode

          Fraction windows                         10 windows MAX.

          Window (Start, End)                    999 min. 59 sec. MAX.

          Fraction capacity                         99 min. 59 sec./tube MAX.

          (Request & Monitor)

Manual Mode

Bottle Simple Mode {Refer to Simple Mode above}

Bottle Peak Mode {Refer to Peak Mode above}

Bottle Window Mode {Refer to Window Mode above}

Range of Wait time setting                    99 min. 59 sec. MAX.

Peak parameter

          Peak level                                     100 % MAX.

          Peak slope                                   10.00 mV/min. MAX.

          Dropper assembly                       9 min. 59 sec. MAX. movement delay time

CONTACT CLOSURE SIGNALS (I/O Terminal)

Event Marker output                              One fractionation marker ON - OFF

Chromatograph signal input                  DC 10 mV (Full Scale)

Ext. Start input                                       ON Start

Ext. End input                                        On End

Ext. Count (Signal) input                       On Count

DIGITAL SIGNAL SPECIFICATION (Serial I/O Terminal)

Serial Interface                                      RS-232C (9 PIN connector)

                                                              1200BPS (START BIT 1, DATA BIT 8, STOP BIT 2)

ELECTRICAL & GENERAL SPECIFICATIONS

Clock                                                     Crystal oscillator

Operation parameter memory              Backed up by continuously recharged battery

Operating temperature                          2 - 40 °C (36 - 104 °F)

Power supply                                         AC 90 V to 260 V, 50/60 Hz

Dimensions                                           W 355 mm x D 340 mm x H 310 mm

Weight                                                   7.0 Kg (15.43 lbs.)