Firma:               Appl.Biosystems

Modell:             ABI 7000

ABI PRism®R 7000 Sequence

Introduction

The ABI PRism 7000 Sequence Detection System is a complete, real-time PCR system that detects and quantitates nudelt acid sequences. In real-time PCR, cycle-by-cycle detection of accumulated PCR product is made possible by combining thermal cycling, fluorescence detection, and application­specific Software in a single Instrument. Quantitative results are available immediately after PCR without additional purification or analysis.

Real-time, quantitative PCR applica­tions include gene expression and

pathogen detection. Post-PCR detection is also available for non-quantitative assays such as allelic discrimination (SNP detection) and plus/minus assays.

Fluorescence Detection

All Sample wells are illuminated using a tungsten-halogen lamp. Fluorescence emission is optimized for use with: FAM-/SYBR® Green 1, VIC/JOE,- TAMRA,- and ROX dyes on a Charge-coupled device (CCD) camera.

Assay Chemistry

Rapid assay development guidelines are provided to ensure success when using the fluorogenic 5" nuclease assay or the SYBR" Green 1 double-stranded DNA binding dyc assay. Rapid assay development guidelines consist of the following steps:

•    Automatically design primers and probes using Primer Expre,ss® Software (included with the 7000 system)

•    Use TaqMan® Universal PCR Master Mix or SYBR- Green PCR Master Mix to provide standardized component concentrations and simplify assay Set-up

•    Use universal thermal cycling pararneters so that multiple assays can be tun on the saure 96-well plate

•    Use default primer and probe concen­trations to eliminate assay optimization

Default primer and probe concentrations are valid for multicolor SNP assays using TaqMan MGB (minor groove binder) probes, and single color quan­titation assays using TaqMan probes or SYBR Green 1 dye detection. Assay optimization is recommended for multiplex quantitation assays to minimize PCR competition.

TaqMan Genomis Assays

Applied Biosystems provides prefor­mulated, ready-to-use, quality-tested, 5" nuclease TaqMan"'probe-based assays for use with the 7000 system.

System Components

7000 Sequence Detector

•      Peltier-based, 96-well block thermal cycling system

•      Tungsten-halogen excitation source

•      Fluorescence detection via a CCD camera

Computer Specifications

Applied Biosystems supplies a Dell Business Line computer (notebook or tower) for use with the 7000 system. For the latent computer Specifications, please visit the Applied Biosystems Web site at www.appliedbiosystems.com

Sequence Detection Software

The Software runs on the Windows® 2000 Operating System and is used for instrument control, data collection, and data analysis. Software Features include:

•      Real-time monitoring during data collection

•      Intuitive multiplex assay Set-up and analysis

•      Simple dissociation curve data collection and viewing

•      Intuitive allelic discrimination viewer, enabling simple allele calling of all Samples on a plate

•      Automaus identification of Samples

containing a PCR inhibitor when per­forming plus/minus assays with an IPC

Installation Specifications

Using the TaqMan® RNase P Instrument Verification Plate, the ABI PRISM 7000 Sequence Detection System can distinguish between samples containing 5,000 and 10,000 template copies with a 99.7% confidence level.

Reagents and Disposabies

A complete live of reagents and dispos­ables is available for use with the ABI PRISM® 7000 Sequence Detection System.

Dimensions

ABI PRisml 7000 Sequence Detection System

Width            39 cm (15.25 in)

Depth            51 cm (19.75 in)

Height           53 cm (20.75 in)

Weight          34 kg (75 lbs)

       Notebook Computer       Tower Computer

Width          33 cm (13 in)                     43 cm (17 in)

Depth         28 cm (11 in)                     59 cm (23 in)

Height         31 cm (12.25 in) (open)     46 cm (18 in)

       5 cm (2 in) (closed)

Weight        3.6 kg (8 lbs)                      32 kg (70 lbs)