manufacturer :       Agilent

model :                   G2445D

annotation :           Dokumente engl.

Agilent 1100 Series LC/MSD Trap VL & SL

Specifications

Mass accuracy (VL & SL)

± 0.2 u within the calibrated standard mass range at normal resolution in full scan mode, with proper calibration ,1CC target and ion statistics, and thermal equilihrium of electronics and ion source.

Mass axis stability (VL & SL)

VVithin ± 0.2 u of the observed calibrated value over 8 hours in standard mass range at normal resolution in full scan mode, with proper ICC target and ion statistics, and ther­mal equilibrium of olectronics and ion source and ambient temperature of 21°C ± 3°C (70°F ± 6°F)

Monoisotopic precursor selection (VL & SL)

Monoisotopic precursor selection is possible throughouttho standard mass range (m/z 50 — 2200) at ambient temperature of 21°C ± 3°C (70°F ± 6°F).

System performance (VL & SL}

Mass Range {m/z)                Resolution (FWHM (u)]     Scan Speed [u/ s{

Standard 50— 2200               Normal ≤ 0.6                        13,000

                                              Enhanced ≤ 0.45                  5,500

                                              Maximum ≤ 0.3                    1,650

Extended 200 — 4000           Normal ~ 3 - 4                      27,000

Polarity switching

VL ~ 2 soconds

SL ~ 0.5 seconds

Sensitivity

Conditions:

Column —ZORBAX Rapid Resolution SB-C18 2.1x30 mm 3.5 micron

Mobile phase — 25% water 75% methanol 5 mM ammonium acetate

Flow rate — 400 µl/min

Mode — full scan MS/MS

MS/MS — transition of the protonated molecular ion (m/z 609) to the sum of the two most abundant product ions

Product ion scan range — m/z 175 - 650

Mass range — standard (m/z 50 - 2200)

Resolution — normal (0.6 u)

            Quantity                    Signal-to-Noise Ratio

VL         25 pg of reserpine       ≥25:1

SL        5 pg of reserpine         ≥25:1

Stages of MS

            Automated                Manual

VL         2 (MS/MS)                  3

SL         5                                 11