Firma:              Agilent

Modell:            3D-Capill.Elek

Kommentar:   Dokumente dt./engl.

Hersteller:                  Hewlett Packard

Typ:                           3D-Capillary Electrophoresis Instrument G1600A

Ausstattung:              HP 3D Kapillarelektrophorese-System:

                                 3D CE-Gerät mit DAD

                                 3D-CE Chemstation-Software

Zubehör:                    HP Vectra XM4 5/150

Überwachung:           gemäß QM-VA 1110-03 Abschnitt 5.2.2., bei jeder Meßreihe

Wartung:                   Reinigung der Elektroden und Prepuncher einmal die Woche.

                                 Austausch der Luftfilter bei Bedarf ( ca. alle 2 Monate)

Reinigung:                 bei Bedarf mit handelsüblichen Reinigern

HP G1602A

HP 3D Kapillarelektrophorese-System einschl. HP 3D CE-Gerät, HP 3D-CE Chem­station-Software, HP Vectra XM4 5/150 HP LaserJet 5- Drucker und Win 95

HP G1314A

HP 1100 Variabler Wellenlängendetektor Mit variablem Wellenlängendetektor mit Deuteriumlampe und CAN-Kabel, Ohne Flußzelle

Standardflußzelle

Flußzelle aus Edelstahl mit 10 mm optischer Weglänge und 14 Mikroliter Volumen. Druckfest

bis zu 40 bar

Specifications

Dimensions                    Width                   42.5 cm, (16.8 in)

                                       Height                  57.5 cm, (22.7 in)

                                       Depth                   52 cm, (20.5 in)

                                       Weight                 52 kg, (115 Ib)

Environment                   Temperature:       5—40 °C

                                       Humidity:              up to 80 %, at 30 °C (non-condensing)

Power requirements       Line voltage:        100/120/220/240 VAC ; +5 %, -10 %, 650 VA

                                       Line frequency:   50 (48—55) Hz; 60 (57—66 ) Hz

Pressure system            Programmable with 0—50 mbar bidirectional

Flushing with 1 bar or with high pressure 2—12 bar bidirectional Vial pressurization with high pressure 2—12 bar

Safety features               Current leak detection; Low current limit

Safety sensors at door and cover disabling high voltage Diagnostic functions

Electrophoresis power   Voltage range: setable 0 to ± 30 kV supply

                                       Current:        setable 0—300 µA

                                       Power:          setable 0—6 W

Operation under constant voltage, current or power Programmable polarity switch

Injection modes              Seif correcting injection system with injection from both ends

                                       Programmable range:             up to 10,000 seconds

                                       Pressure:                                 0—50 mbar

                                       Electrokinetic:                          0—30 kV

Autosampler/                  48-position carousel. All vials are randomly accessible from

fraction collector             cathode and anode end of capillary. Temperature control with external waterbath with vial temperature from 10—40 °C (non-condensing conditions)

Replenishment               Satellite Station for refilling anode and cathode buffer vials with fresh buffer for automatic continuous operation and buffer selectable buffer levelling

Vials                                100 µl sample vials, 1 ml or 2 ml buffer vials (polypropylene or glass) with resealing snap caps

Capillary cassette           Forced-air temperature-controlled with Peltier element

                                       Temperature range:                      10 °C below ambient to 60 °C (± 0.1 °C) with a min. of 4 °C

                                       Minimum total capillary length:      33 cm

                                       Capillary compatibility                   365 µm o.d.

Detector                          Real time UV-Visible diode-array detector (190—600 nm)

                                       Wavelength accuracy:             1 nm

                                       Response time:                       0.1 to 10 s (8 choices)

                                       Light source:                            prealigned deuterium lamp

                                       Signals:                                    up to five signals simultaneously, full spectral acquisition with Agilent ChemStation

Raw data channels        Detector signals, voltage, current, power, capillary temperature and pressure.

System control               Operating with graphical user interface under Microsoft° Windows 95 and Windows NT

                                       Time programmable parameters:

voltage, current, power, polarity pressure, inlet and outlet vial capillary temperature, pre and post-run conditioning with pressure and/or voltage, replenishment, fraction collection

CE specific software      Mobility report, time corrected areas, pl calibration and bio polmer

                                       size calibration

CE — for highest confidence in your results

Typically, there are five steps required for full instrument, method and data validation. Agilent Technologies offers software tools and kits to facilitate and automate there steps for the Agilent CE system.

Step1: Qualify your vendor and your vendor's design

Ensured with Agilent's reputation and documentation of successful development validation.

Step 2: Qualify the instrument in your laboratory before beginning operation

Follow the appropriate test procedures and certification for Installation Qualifica­tion (IQ) and Operation Qualification/ Performance Verification (OQ/PV). Agilent offers a kitwhich provides instructions, methods and materials for performing OQ/PV for the Agilent CE system.

Step 3: Validate your analytical method

The Agilent ChemStation includes system suitability software for automated method validation.

Step 4: Qualify system performance during routine operation

Agilent's software features automated daily system suitability testing, with an optional database with online quality control charts for documenting perfor­mance parameters.

Step 5: Ensure data security, integrity and traceability

The software has a password-protected user access. lt saves instrument condi­tions and logbooks together with raw data in checksum-protected binary fites.