The Xuri Cell Expansion System W25 provides an automated, controlled environment for expanding cell therapies in volumes up to 25 liters, suitable for research, process development, and manufacturing. The closed system minimizes the risk of cell contamination. Higher cell densities are achieved through automated perfusion protocols. The process remains controllable thanks to advanced control functions and remote monitoring via UNICORN software. It is suitable for use in regulated environments. It enables cell counts exceeding 10 x 106 cells/mL in volumes up to 25 liters, with non-invasive mixing, media perfusion, and efficient oxygen transfer in a gentle, low-shear environment. External air supply per CBCU: 1.0 to 1.5 bar; normal consumption 1.3 L/min; rapid fill 3.5 L/min. External CO2 supply per CBCU: 1.0 to 1.5 bar; normal consumption 0.2 L/min. Rapid filling 0.5 L/min. External O2 supply per CBCU: 1.0 to 1.5 bar; normal consumption 0.7 L/min; rapid filling 1.7 L/min. Shaking specifications: speed adjustable from 2 to 40 rpm, shaking angle from 2° to 12°, speed profile from 15% to 100% (15% means nearly constant angular velocity; 100% corresponds to sinusoidal shaking). Media weight adjustable from 0.5 to 25 kg, scale accuracy in single mode ±(0.050 + 1% of the load) kg. Pt100 Class A temperature sensor, measuring range 2 °C to 50 °C, maximum setpoint difference in dual mode 10 °C at an ambient temperature of 21 °C, with a reduction of 1 °C per degree above 21 °C, control range from ambient temperature +5 °C to 40 °C, control accuracy ±0.2 °C excluding measurement errors. CBCU specifications: Gas flow adjustable from 50 to 1000 mL/min with a total deviation of ±(10 + 3 % of the measured value) mL/min, rapid filling approx. 3 L/min. CO2 control range 0% to 15%, measuring accuracy at 5% CO2 ±0.5% (mixed with air/N2 only), control accuracy ±0.4%. Oxygen control range 0% to 50% O2 (mixed with N2) or 21% to 50% O2 (mixed with air). O2 measurement accuracy ±(0.6 + 1% of the reading), control accuracy ±0.6%. pH measurement range 4.5 to 8.5 with measurement accuracy ±0.05 pH within ±0.25 pH calibration deviation, pH control range 6.0 to 8.0, control accuracy ±0.05 pH. Oxygen saturation (DO) measurement range 0% to 250% air saturation, measurement accuracy ±5% (without pressure variation), control range 0% to 100%. Pump specifications: flow rate range 0.1 to 144 L/day (0.07 to 100 mL/min), accuracy ±(0.1 + 5% of the reading) mL/min after calibration, cumulative volume accuracy ±10%. Supported tubing dimensions: inner diameter 0.5 to 4.8 mm, wall thickness 1.6 mm. Dimensions: Shaker 404 x 205 x 560 mm, CBCU 276 x 117 x 360 mm, pump 275 x 115 x 280 mm. Weights: Shaker 24 kg, CBCU 4.8 kg, pump 3.8 kg. Software not included.