Citometria a Flusso

Becton Dickinson FACSCalibur

13.600,00 €
Excl. 19% VAT , excl. Shipping Cost
16.184,00 € incl. Vat
Seriennummer: 32380
Stato: Stock unit?The device is in our stock, however, has to be inspected by our technician, in order to be able to guarantee complete function.
Spedizione: 4-6 weeks
Descrizione:
Becton Dickinson Citometro a flusso FACSCalibur.
Con laser rossi e blu.
Selezionatore di cellule con cella di flusso/separazione.
5 rilevatori. Modulo concentratore.
Campionatore HT per MTP.
Sistema di alimentazione del flusso FACS.
Convertitore di tensione 115/220 V.
Add to Offer list
Maggiori Informazioni
Objektnummer B00032380
Seriennummer 32380
Nome oggetto Becton Dickinson FACSCalibur
Stato Lagergerät
Fabbricante Becton Dickinson
Spedizione 4-6 weeks

Status, terms of delivery and payment

Verification of devices

The second-hand devices are verified by Labexchange Service GmbH before delivery. You are receiving only fully functional devices.

Dispatch time

The stated dispatch times are the shortest possible ones for each article. The effective dispatch times can vary. The effective dispatch times will be stated in the order confirmation.

As a matter of principle, we are offering collective deliveries. The shipping time is calculated based on the position with the longest lead time. A partial delivery is possible on explicit request.

 

Shipping methods

Parcel services, forwarding agencies, self-pickup, delivery by Labexchange fleet.

 

Delivery information

Prices exclude shipping costs. Stated shipping costs are to be expected. Deviations are possible. If transport costs are not specified, please ask separately for them.

For deliveries to countries outside the EU, an additional fee of 70,00€ for export is charged. Note for international shipments: A proof of preference/EUR1 will not be issued by us. When self-collecting/ordering EXW from countries within or outside the European Union, 16% VAT will be retained as a deposit until we have received the corresponding confirmation of arrival/bill of delivery from the buyer.

 

Terms of payment

We do not accept payment by credit card, letter of credit, PayPal, etc. In each case the invoice amount is payable without deduction. Discount is not granted.

Country

Possible payment methods

Comment

DE, AT, CH

Payment by invoice, payment in advance

Payment by invoice is only possible for corporate clients.

NL, BE, LU

Payment by invoice, payment in advance

Payment by invoice is only possible for corporate clients

Other countries

Payment in advance

 

 

Our General Terms of Sale, Delivery and Payment are valid and are available for download here. 

The goods are offered subject to prior sale.

 

Definition of status

All articles are used articles, except an article is listed especially as a new device.

Status

Condition

Comment

Immediately available

Used The article is fully functional and in impeccable condition. It can be shipped immediately.
In stock

Used

The article is on stock. Our service technicians will verify the article before delivery. You are receiving only a fully functional article.

Published

Used

The article is still with the provider. After your order the article will be purchased and verified by us before being shipped to you. A certificate of operativeness as well as a service report are included in delivery.

New device

new

The article is brand new and unused. Regarding new equipment the guarantee/warranty conditions of the corresponding manufacturer apply.

manufacturer:      Becton Dickinson

model:                  FACSCalibur

 

 

The following illustrations and descriptions refer to the instrument model and are drawn from brochures. They do not represent the scope of delivery. Please refer to the text of the offer for the exact scope of delivery.

 

 

 

 

 

 

Optics

 

Excitation Optics

 

Optical platform

Fixed optical assembly

 

Lasers

Air-cooled argon-ion laser, 15 milliwatt, 488 nm; life expectancy >5,000 hours.

Optional second laser: nominally 635 nm.

 

Beam geometry

Prismatic expander and achromatic

spherical lens providing 22 x 66-μm

elliptical beam for argon-ion laser.

Nominally 15 x 61-μm elliptical beam

for red-diode laser.

 

 

 

Emission Optics

 

Optical coupling

Quartz cuvette coupled to emission lens by refractive index matching optical gel for optimum collection efficiency.

 

Background rejection

Obscuration blade and slit for minimizing unwanted laser radiation at the detector.

 

Forward scatter detector and filter

High-performance solid state silicon detector with 488-nm bandpass filter for clear signal detection and red-diode (635-nm) laser signal rejection.

 

Side scatter detector

High-performance photomultiplier using Brewster-angle beam splitter in the emission optical train.

 

Fluorescence detectors and filters

Up to four high-performance, high dynamic range photomultipliers with bandpass filters: 530 nm (FITC), 585 nm (PE/PI), and >670 nm (PerCP) with base unit, and optional 661 nm (APC) with FL4 option.

 

 

 

Performance

 

Fluorescence

 

Fluorescence Sensitivity

Estimated detection limit: 750 molecules of equivalent soluble fluorescein.

 

Fluorescence resolution

Coefficient of variation in FL2-Area of <3%, full peak for propidium iodidestained chicken erythrocyte nuclei.

 

 

Forward and Side Scatter

 

Forward and side scatter sensitivity

Separation of fixed platelets from noise.

 

Forward and side scatter resolution

Optimized scatter performance for resolving lymphocytes, monocytes, and granulocytes.

 

 

Signal Processing

 

Workstation resolution

1,024 channels on all parameters.

 

Dynamic range

Logarithmic amplifiers for SSC, FL1, FL2, FL3, and FL4 (with FL4 option), providing four log decade range.

 

Fluorescence compensation networks

Compensation for fluorescence spectral overlap between FL1 and FL2, between FL2 and FL3, and between FL3 and FL4

channels (with FL4 option).

 

Pulse processing

Width and area measurements for discriminating doublets; available for all fluorescence parameters.

 

Time

Time available correlated to any parameter for kinetic experiments or other applications.

 

 

 

Fluidics

 

General operation

Front key panel control in three modes:

RUN, STNDBY, and PRIME; automatic standby mode for conserving sheath fluid by stopping sheath flow when no sample tube is installed.

 

Fluid reservoirs

Easily accessible 4-L capacity sheath and waste containers housed in a convenient pull-out drawer; level detectors for automatically indicating low levels of sheath or high levels of waste.

 

Sample flow rates

Three selectable flow rates of 60 μL/min, 35 μL/min, and 12 μL/min; regulated and monitored pressure difference between sheath and sample; particle velocity in flow cell: approximately 6 meters/second.

 

Quartz cuvette

Internal cross-section: rectangular 430 x 180 μm; antireflection coated external surfaces for maximum transmission of laser light.

 

Sample concentration

Single-cell suspension of 105 to 2 x 107 particles/mL recommended range.

 

 

 

Data Management

 

Workstation

BD FACStation™ Mac Pro computer

 

Processor

2.8-GHz Quad-Core Intel® Xeon® processor

 

Memory

2 GB RAM

 

Level 2 cache

4 MB per processor

 

Data storage

320-GB 7200-rpm Serial ATA, 3Gb/s

 

Networking

On-board Ethernet, built-in AppleTalk® Networking, and Apple® File Sharing

 

Optical drive

16x SuperDrive

 

Monitor

17-inch LCD monitor (optional)

 

Data file structure

Flow Cytometry Standard (FCS) 2.0/3.0

ASCII results file for data export

 

 

 

Sample Loading

 

Sample delivery

Tube-lifter design with multiple sensors that verify rack identification and tube position with optional BD FACS™ Loader.

 

Rack capacity

40 (12 x 75-mm) tubes per rack.

 

Rack support

Up to 16 racks per BD FACS Loader.

 

Data entry

Sample information, reagent panels, and rack information can be defined for up to 640 tubes (40 tubes x 16 racks) at a time.

 

Loader manager

Automated control through BD™ Worklist Manager software.

 

Barcode scanner (optional)

Automates data entry for Codabar, Code 39, Interleaved 2 of 5, Code 2 of 5, and Code 128.

 

Mixing mode

Adjustable high-energy and low-energy mix.

 

 

 

Installation requirements

 

Dimensions (W x D x H)

 

Sensor module

91.4 x 61.5 x 67.3 cm

(36 x 24.2 x 26.5 in)

 

Height with cover option

124.5 cm (49 in)

 

Computer

48 x 41 x 58 cm (19 x 16 x 23 in)

 

Printer

48 x 41 x 58 cm (19 x 16 x 23 in)

 

 

Weight

 

Sensor module

109.1 kg (~240 lb)

 

Computer

varies with configuration

 

 

Power

US: 120 VAC ±10%, 50/60 Hz ±2 Hz,

Current: 20 amp maximum outside

US. External transformer needed for

100 VAC ±10%, 50/60 Hz ±2 Hz, and

220/240 VAC ±10%, 50/60 Hz ±2 Hz.

 

 

Water supply

None required

 

 

Air supply

None required

 

 

Heat dissipation

BD FACSCalibur system with all options

6,500 BTU/h

 

 

Temperature

16–29°C (60–85°F)

 

 

Humidity

10% to 90% relative non-condensing

 

 

Air filtering

No excessive dust and smoke

 

 

Lighting

Optics and detectors shielded from room

lighting

 

Gruppo prodotti: ZYTO

Back to Top
part-of-labexchange-group
Search engine powered by ElasticSuite
Labprocure