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24 months legal warranty
60 days Labexchange Fullguarantee
Certificate of operativeness
The Labexchange right of return
Optional installation of equipment for immediate and safe use of equipment
Questions regarding this device
If you buy this item used instead of new, you save as much CO2 as driving 1850 km with a gasoline car.
The legal warranty period for our devices is 24 months from delivery date.
All used instruments of Labexchange are subject to a technical inspection before delivery and will be supplied only, when they function perfectly well. The verification and functionability is documented by a service report of an independent service company. The Labexchange Fullguarantee is 60 days from delivery date. Malfunctions occurring within the warranty period will be corrected by us free of charge. (Valid for countries within the EU and CH)
Our certificate of operativeness ensures a technically perfect condition.
You are on the safe side with our used laboratory and analysis systems! For even more security during the purchase, we are granting the Labexchange right of return.
Return your laboratory or analysis system simply without explanation.
Our special terms and conditions are to be applied:Labexchange right of return(https://www.labexchange.com/fileadmin/user_upload/pdf/downloads/en/Rueckgaberecht_08_18_E.pdf)
On demand, we verify the option of an extension of warranty for your device. If possible, the period of warranty is 6 months for 10 % extra charge and 1 year for 20 % extra charge on the list price. (Valid for DE, AT, CH, FR, NL, BE, LU, GB and IR)
Play it safe! We are pleased to offer you the installation of your laboratory equipment. Our qualified technicians install the device on your desired date in the laboratory, so that you can work quickly and safely with your new laboratory equipment.
The installation of the equipment by Labexchange brings further advantages. You thereby obtain a guarantee extension to 6 months and you have also the right to the Labexchange Repurchase Guarantee. You receive sensible laboratory equipment and analytical systems as well as much of the available laboratory hoods, already incl. installation.
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The second-hand devices are verified by Labexchange Service GmbH before delivery. You are receiving only fully functional devices.
The stated dispatch times are the shortest possible ones for each article. The effective dispatch times can vary. The effective dispatch times will be stated in the order confirmation.
As a matter of principle, we are offering collective deliveries. The shipping time is calculated based on the position with the longest lead time. A partial delivery is possible on explicit request.
Parcel services, forwarding agencies, self-pickup, delivery by Labexchange fleet.
Prices exclude shipping costs. Stated shipping costs are to be expected. Deviations are possible. If transport costs are not specified, please ask separately for them.
For deliveries to countries outside the EU, an additional fee of 70,00€ for export is charged. Note for international shipments: A proof of preference/EUR1 will not be issued by us. When self-collecting/ordering EXW from countries within or outside the European Union, 16% VAT will be retained as a deposit until we have received the corresponding confirmation of arrival/bill of delivery from the buyer.
We do not accept payment by credit card, letter of credit, PayPal, etc. In each case the invoice amount is payable without deduction. Discount is not granted.
Country
Possible payment methods
Comment
DE, AT, CH
Payment by invoice, payment in advance
Payment by invoice is only possible for corporate clients.
NL, BE, LU
Payment by invoice is only possible for corporate clients
Other countries
Payment in advance
Our General Terms of Sale, Delivery and Payment are valid and are available for download here.
The goods are offered subject to prior sale.
All articles are used articles, except an article is listed especially as a new device.
Status
Condition
Immediately available
Used
The article is on stock. Our service technicians will verify the article before delivery. You are receiving only a fully functional article.
Published
The article is still with the provider. After your order the article will be purchased and verified by us before being shipped to you. A certificate of operativeness as well as a service report are included in delivery.
New device
new
The article is brand new and unused. Regarding new equipment the guarantee/warranty conditions of the corresponding manufacturer apply.
manufacturer: Becton Dickinson
model: LSR II
annotation: Dokumente engl.
The following illustrations and descriptions refer to the instrument model and are drawn from brochures. They do not represent the scope of delivery. Please refer to the text of the offer for the exact scope of delivery.
BD TM LSR II is the first and only air cooled four-laser benchtop flow cytometer with the ability to acquire 10 or more colors. At the heart of the BD LSR II is the new digital acquisition system, sampling signals at 10 million times a second, providing increased detection and channel resolution per pulse, thus yielding more information per cell. BD digital software provides both online and offline compensation running in a Windows®- based environment. Configure your cytometer with fixed-alignment 488-nm, 633-nm, 405-nm, and 355-nm lasers. Each laser has a spatially separated beam spot. Light is transmitted by fiber optics to an innovative Octagon detector array with user-interchangeable reflective dichroics and optically matched bandpass filters that produce increased sensitivity. Multicolor experiments have now gotten easier by running them on the BD LSR II.
Specifications*
Performance
Fluorescence Sensitivity
Estimated detection limit is <200 molecules of equivalent soluble fluorescein (using Spherotech Rainbow RCP 30-5A particles).
Fluorescence Resolution
Coefficient of variation FL2-Area of <3%, full G 0 /G 1 peak for propidium iodide (PI)stained chicken erythrocyte nuclei
Fluorescence Linearity
Doublet/singlet ratio for CENs stained with PI = 1.952.05 detected off the 488-nm or UV laser
Forward and Side Scatter Sensitivity
Sensitivity enables the separation of fixed platelets from noise.
Forward and Side Scatter Resolution
Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.
Sample Acquisition Rate
>20,000 events/sec (2 x 10 7 cells/mL running at 60 µL/min)
Excitation Optics
Optical Platform
Fixed optical assembly
Kinematic beam steering
Lasers
20-mW; Coherent Sapphire 488-nm, air-cooled, argon-ion laser
25-mW; 405-nm Coherent VioFlame
20-mW; 635-nm JDS Uniphase HeNe
355-nm Lightwave Solid-State laser
Beam spot size
15 ±3-µm height and no greater than 75 ±15-µm width
Emission Optics
Optical Coupling
Quartz curvette is coupled to emission lens by refractive index-matching optical gel for optimum collection efficiency.
Fluorescence Detectors and Filters
Four fixed fiber apertures
(125-µm separation)
Ten fluorescence detectors
High-performance, high dynamic range photomultiplier and red‑sensitive photomultipliers with filters
Four wavelengths detected from the 488-nm laser:
515545 nm FITC
562588 nm PE
675715 nm PerCP Cy5.5 or PE-Cy5.5
750810 nm PE-Cy7
Two wavelengths from the 635-nm laser:
650670 nm APC
750810 nm APC-Cy7
Two wavelengths from the 325-nm laser:
395415 nm Indo-1 (Violet)
425475 nm DAPI or Hoechst
Two wavelengths from the 405-nm laser:
420460 nm Cascade Blue
505585 nm Alexa 430
Additional bandpass filters supplied:
663677 nm PE-Cy5
515545 nm Indo-1 (Green)
Filters and mirrors are user changeable.
Signal Processing
Workstation Resolution
Five decades for peak detection: offset less than 1/10,000 of full scale
Data Acquisition Channels
Twelve acquisition channels with up to four time-displaced laser beams
Dynamic Range
18-bit data acquisition
262,144 linear channels displayed in a 5-decade logarithmic display
Fluorescence Compensation Networks
Inter- and intra-beam software- programmable resolution of 0.01%
Pulse Processing
Width and Area measurements available for all fluorescence parameters
Ratio measurements for intra-laser parameters (useful for calcium measurements)
Time
Time available correlated to any parameter
Channel Trigger
Triggering on any laser signal
Ability to create logical AND/OR of channel triggers
Ratio
Ratio measurements between any two parameters
Fluidics
General Operation
Front key panel provides three modes: RUN, STNDBY, and PRIME;
automatic standby mode conserves sheath fluid by stopping sheath flow when no sample tube is installed.
Sample Flow Rates
Three selectable flow rates
Regulated pressure difference between sheath and sample
Quartz Curvette
Internal cross-section is rectangular 430 x 180 µm; external surfaces are anti-reflection coated for maximum transmission of laser light.
Data Management System
Workstation
HP X4000 workstation or equivalent
Central Processing Unit (CPU)
Dual Intel Xeon processors
Memory
2 GB RD RAM
Data Storage
Nine-and 36-GB internal hard drive, internal drive with 250-MB removable disk storage space capacity, 3.5-inch floppy drive, CD-RW drive, tape drive
Networking
Ethernet
Monitor
Two 18-inch LCD monitors
Printer
Networkable color-inkjet printer
Data File Structure
Flow Cytometry Standard (FSC) 3.0 or 2.0
Your Labexchange-Team will gladly help:
Christian Schmid Laboratory and analysis, Laboratory equipment, Life science
Hubert Sauter Spectroscopy, Chromatography