Becton Dickinson FACSJazz
2 laser system (4B/2R).
488nm blue laser and 640nm red laser.
Detector blocks.
BD FACS software.
VAC & AIR compressor.
| Objektnummer | B00042631 |
|---|---|
| ID-number | 42631 |
| Object name | Becton Dickinson FACSJazz |
| Manufacture | Becton Dickinson |
| Shipping | 4-6 weeks |
| Status | Provider |
Product group: ZYTO
Status, terms of delivery and payment
Verification of devices
The second-hand devices are verified by Labexchange Service GmbH before delivery. You are receiving only fully functional devices.
Dispatch time
The stated dispatch times are the shortest possible ones for each article. The effective dispatch times can vary. The effective dispatch times will be stated in the order confirmation.
As a matter of principle, we are offering collective deliveries. The shipping time is calculated based on the position with the longest lead time. A partial delivery is possible on explicit request.
Shipping methods
Parcel services, forwarding agencies, self-pickup, delivery by Labexchange fleet.
Delivery information
Prices exclude shipping costs. Stated shipping costs are to be expected. Deviations are possible. If transport costs are not specified, please ask separately for them.
The stated transport and packing charges apply to the most favorable route if transport and are to be understood as subject to verification due to unexpected cost increases. By reason of unpredictable events, cargo rates and delivery times can change at any time and therefore have to be adapted to the recent situation. Import formalities and possible customs charges will be borne by the purchaser. Incoterm coding according to Incoterms 2010: For persons who collect the devices themselves: EXW, for dipatch by sea: CFR, by air freight: CPT, other shipments: DAP. Note for international shipments: A proof of preference/EUR1 will not be issued by us. When self-collecting/ordering EXW from countries within or outside the European Union, 16% VAT will be retained as a deposit until we have received the corresponding confirmation of arrival/bill of delivery from the buyer.
Terms of payment
We do not accept payment by letter of credit, PayPal, etc. In each case the invoice amount is payable without deduction. Discount is not granted.
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Country |
Possible payment methods |
Comment |
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DE, AT, CH |
Payment by invoice, payment in advance, paymet by credit card |
Payment by invoice is only possible for corporate clients. |
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NL, BE, LU |
Payment by invoice, payment in advance, payment by credit card |
Payment by invoice is only possible for corporate clients |
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Other countries |
Payment in advance, payment by credit card |
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Our General Terms of Sale, Delivery and Payment are valid and are available for download here.
The goods are offered subject to prior sale.
Definition of status
All articles are used articles, except an article is listed especially as a new device.
|
Status |
Condition |
Comment |
|
Immediately available |
Used | The article is fully functional and in impeccable condition. It can be shipped immediately. |
| In stock |
Used |
The article is on stock. Our service technicians will verify the article before delivery. You are receiving only a fully functional article. |
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Published |
Used |
The article is still with the provider. After your order the article will be purchased and verified by us before being shipped to you. A certificate of operativeness as well as a service report are included in delivery. |
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New device |
new |
The article is brand new and unused. Regarding new equipment the guarantee/warranty conditions of the corresponding manufacturer apply. |
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Labprocure |
Used |
Labprocure GmbH, as the advertiser, is responsible for the content of this device offer. Labprocure assumes liability for the offers advertised here and for the photos and offer texts included. Labprocure GmbH, Bruckstraße 58, 72393 Burladingen. |
manufacturer: Becton Dickinson
model: FACSJazz
The following illustrations and descriptions refer to the instrument model and are drawn from brochures. They do not represent the scope of delivery. Please refer to the text of the offer for the exact scope of delivery.
Technical Specifications
Installation Requirements
Dimensions (H x W x D)
Cytometer: 53.3 x 50.8 x 50.8 cm (21 x 20 x 20 in.)
Table (optional): 94.0 x 121.9 x 81.3 cm. (37 x 48 x 32 in.)
Computer cart (optional): 94.0 x 71.1 x 66.0 cm (37 x 28 x 26 in.)
Biological safety cabinet (optional): 233.4 to 249.9 x 136.5 x 87.6 cm (91.9 to 98.4 x 53.8 x 34.5 in.)
Weight
Sort head does not exceed 56.6 kg (125 lb)
Cytometer system, excluding options, does not exceed 151.5 kg (334 lb)
Temperature Operating Range
15ºC–25ºC (59ºF–77ºF)
Temperature changes within this range can affect the surface tension of fluids, and may require the operator to make minor adjustments to the system.
Power
One dedicated power line:
100/120/220/240 VAC, 50–60 Hz
Optional biological safety cabinet, aerosol management option, sample temperature control, air compressor, and vacuum supply may require an additional line. Power consumption: <600 VA
Humidity
55% ±10% relative humidity (non-condensing)
Heat Dissipation
The BTU ratings at 120 VAC for the components are as follows:
Cytometer: 1,638 BTU or 480 Whr
Air compressor (optional): 3,275 BTU or 960 Whr
Vacuum pump (optional): 1,720 BTU or 504 Whr
Sample temperature control (optional): 4,709 BTU or 1,380 Whr
Air Supply
A laboratory air source of compressed air must deliver clean (less than 5 PPM), dryfiltered (oil-free) air with stable pressure of 40–90 psi (2.5–6.2 bar) regulated. An air source option is available.
Vacuum Supply
A laboratory vacuum source must supply between 5–15 in. Hg at 1 CFM.
A vacuum source option is available.
OPTICS
Excitation Optics
Optical Platform
Each laser beam is aligned independently and has its own final focus lens foroptimal tuning.
The 488-nm laser comes standard. The 405-nm laser, 561-nm laser, and 640-nm laser are optional.
Power Out of the Laser Head
405 nm: ≥50 mW. 488 nm: ≥80 mW. 561 nm: ≥75 mW. 640 nm: ≥50 mW
Emission Optics
Steering Optics
Emission light is collected through a 20X, 0.6 NA microscope objective. Light is focused on spatially separated mirror pinholes. Simultaneous video observation of the stream, pinholes, and laser intercepts allows for fast and intuitivealignment.
Forward Scatter Detector and Filters
Collected using 75- and 50-mm lenses and a photomultiplier tube (PMT) with a 488/10 bandpass filter and an OD3 neutral density filter.
Side Scatter Detector and Filter
Collected through the 90° collection lens and measured using a PMT with a 488/10 bandpass filter.
Fluorescence Detectors and Filters
Collected through the 90° collection lens and measured using PMTs.
Fluidics
General Operation
- The fluidics system uses external air pressure and vacuum.
- An optional air pressure supply and/or vacuum pump is available.
- Sheath pressure is fixed at 27 psi (1.8 bar). Consumption of sheath fluid is approximately 0.5 L per hour.
Fluidics Reservoirs
Autoclavable 7-L sheath and waste containers, equipped with pressure and vacuum readout, are provided.
Fluidics Control
- A sample flow fine adjustment is provided for precise regulation of the sample flow.
- Purge, pulse, rinse, and run functions are provided for quick stream startup and bubble removal.
Nozzles
100 μm (2 included with the instrument)
Replaceable Fluidics Path
- The fluidics path, including the nozzle assembly and sample line, can be exchanged. There are no inline valves. Only pinch valves are used.
Bubble Detector
A bubble detector in the sample line detects air bubbles from the sample tube and stops sample flow when the sample tube is empty, preventing air bubbles from reaching the nozzle assembly.
Sample Input
- Recommended minimum sample volume is 0.2 mL. The dead volume is approximately 25 μL.
- 12 x 75-mm polypropylene tubes
Performance
Fluorescence Sensitivity
Fluorescence sensitivity was measured using SPHERO™ Rainbow Calibration
Particles according to the manufacturer’s specifications:
FITC: 125 molecules of equivalent soluble fluorochrome (MESF-FITC)*
PE: 125 molecules of equivalent soluble fluorochrome (MESF-PE)*
Fluorescence Resolution
Measured with propidium iodide (PI)
-stained chicken erythrocyte nuclei (CEN) and Pulse Processing/Integrator option. Coefficient of variation (CV) of PI fluorescence area: <3.0% full G0/ G1 peak. Use of the integrator option enables differentiation of G2/M singlets from G0/G1 doublets for PI stained calf thymus nuclei (CTN).
Fluorescence Linearity
Doublet/singlet ratio: 1.95–2.05 for CEN stained with PI
Forward and Side Scatter Sensitivity
Sensitivity enables separation of fixed platelets from noise, and detection of 1.0-μm beads.
Forward and Side Scatter Resolution
Scatter resolution enables differentiation of lymphocytes, monocytes, and granulocytes.
Sort Performance
Drop Drive Frequency
37–41 kHz
Purity and Yield
At 27 psi and 39 kHz with an average threshold of 10,000 events per second, a two-way sort achieved a purity of 98% and a yield of >80% of Poisson’s expected yield for both populations. Higher thresholds up to 20,000 events per second can be achieved without affecting purity; however, yield will decrease based on Poisson’s statistics.
Viability
Sorts of Jurkat cells resulted in >90% viability and similar proliferation rates * MESF
Data Management
Software
BD FACS Sortware sorter software
Workstation
PC workstation with at minimum: Intel® 3.3GHz Quad Core CPU, Microsoft®Windows® 7 Professional 64-bit operating system
Memory
8 GB of RAM
Data Storage
2 x 300 GB hard drives, RAID 1 (mirrored) configuration 8X DVD+/-RW 7 USB 2.0
Networking
(10/100/1000) GB Ethernet
Monitor
27-inch LCD, 2560x1440 resolution
