Nanogen NanoChip
Objektnummer | B00014365 |
---|---|
Seriennummer | 014365 |
Object Naam | Nanogen NanoChip |
Status | Archiv |
Status, leverings- en betalingsvoorwaarden
Apparatuurcontrole
De gebruikte apparatuur wordt voorafgaand aan levering gecontroleerd door Labexchange Service GmbH. U ontvangt volledig functionerende apparatuur.
Verzending
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In de regel bieden we combinatieleveringen aan. Levertijden zijn afhankelijk van het artikel met de langste levertijd. Deelleveringen zijn mogelijk tegen een toeslag.
Verzendmethoden
Koeriersdiensten, transportbedrijven, zelf afhalen, levering door Labexchange wagenpark
Informatie levering
De prijzen zijn exclusief verzendkosten. De genoemde verzendkosten zijn de te verwachten kosten. Afwijkingen zijn mogelijk. In het geval geen kosten voor verzending zijn gespecificeerd, vraag die dan afzonderlijk aan.
De opgegeven vracht- en verpakkingskosten hebben betrekking op de goedkoopste transportroute en zijn onder voorbehoud van onvoorziene kostenstijgingen. Door onvoorziene gebeurtenissen kunnen vrachttarieven en levertijden op elk moment veranderen en moeten ze worden aangepast aan de huidige situatie. Incoterm coderingen volgens Incoterms 2010: Bij afhalen EXW, CFR voor zendingen over zee, CPT per luchtfracht, andere zendingen DAP. Opmerking: We geven geen preferentieel certificaat/EUR1 af. Bij zelf afhalen/af fabriek (EXW) uit derde landen en de EU wordt 16% btw als borg ingehouden, tot we de ontvangstbevestiging/het leveringscertificaat van de koper hebben ontvangen.
Betalingsvoorwaarden
Wij accepteren geen betalingen Letter of Credit, PayPal etc. Het factuurbedrag is volledig verschuldigd. Er zijn geen betalingskortingen. De goederen blijven tot volledige betaling ons eigendom.
Land |
Mogelijke betaalmethoden |
Opmerking |
Duitsland, Oostenrijk, Zwitserland |
Betaling via factuur, vooruitbetaling, per creditkaart |
Betaling via factuur is mogelijk voor ondernemingsklanten. |
Nederland, België en Luxemburg |
Betaling via factuur, vooruitbetaling, per creditkaart |
Betaling via factuur is mogelijk voor ondernemingsklanten. |
Andere landen |
vooruitbetaling, per creditkaart |
|
Onze Algemene Voorwaarden voor Verkoop, Levering en Betaling zijn hierop van toepassing. Deze voorwaarden zijn hier te downloaden.
Tussenverkoop is ons voorbehouden.
Beschrijving status:
Alle artikelen zijn gebruikte artikelen, tenzij bij een artikel uitdrukkelijk wordt vermeld dat het om een nieuw apparaat gaat.
Firma: Nanogen
The following illustrations and descriptions are referring to the instrument model and are drawn from brochures. They are not representing the delivery volume. The exact delivery content you will find only in the offering text.
NanoChip System Components
The NanoChip system includes the Loader, the Reader, NanoChip cartridges, data visualization software, reagent bottles, conductivity meter, start-up kit, user's guide and applications notebook.
NanoChip Loader
Users design and create their own assays or experiments on blank NanoChip cartridge using the Loader. A 96-/384-well plate containing up to 96 or 384 different genetic sequences is placed in the Loader. The Loader then automatically electronically addresses the microarray according to a user-defined map. For experiments requiring further hybridization of complementary genetic material, the Loader can be used to automatically hybridize these samples. The elecronically-driven arraying process is both fast and flexible, allowing for multiple types of experiments.
Note: Build 4 NanoChip Loaders (with Loader serial numbers beginning wich 0137-0xx or 0237-0x.x) and Build 5 Loaders (0295-0xx) have the added feature of a reagent pack slot, which allows the user to load only samples from a 96-/-384-well plate and bad assay reagents from a reagent pack.
NanoChip Reader
The Reader is used to hybridize and analyze target samples using NanoChip cartridges prepared on the Loader A reporter probe is hybridized to matching target sequences on the NanoChip cartridge. Results are detected on the Reader through laser-driven fluorescence.
NanoChip® Cartridge
The NanoChip cartridge encloses the electronic microarray and directs fluid to the chip test surface.
The bar-coded cartridge is available in a ready-to-address Format with no DNA probes attched.
Store all cartridges at 2-8°C until ready for use.
Notes: When handling the cartridge, hold by the clear plastic only, clo not touch the electrical contact area on the underside of the cartridge, do no allow the underside of the cartridge to get wet and do not place the cartridge on any surface > 25°C (see Section X-Operating Safety for detailed information on cartridige handling). Use the NanoChip Cartridge Case to hold cartridges.
Remove the protective tape from the underside of the cartridge before inserting the cartridge into the Loader
NanoChip Electronic Microarray
The NanoChip electronic microarray is the active surface where the electronic addressing takes place. There are 100 individual test sites. Each test site is connected to an electrical contact on the bottom side of the NanoChip cartridge. This contact makes the electrical contact to the Loader or the Reader.
RLAB NanoChp Data Viewer and Anaysis Software
Easy-to-use data analysis tools are provided with the NanoChip System. These menu-driven tools transform the data output into useful information in the form of histograms, tables and data charts that are accessible through the Reader or an internet browser from any networked laboratory computer.
Reagent Bottles
Nanogen provides 11 reagent bottles with the workstation-3 to be used with the Loader and 8 to be used with the Reader.
Conducitvity Bottles
It is critical for the proper operation of the NanoChip System that the histidine buffers remain at the appropriate conducitvity and that samples are low in salts. A conductivity meter is provided to check conductivity of the histidine buffer and samples.
Start-up Kit
Nanogen provides an initial amount of L-histidine powder, high salt buffer, low salt buffer, dH2O, 96-/384-well plates, a Zip disk, NanoChip Microplate Seals (tape to cover the 96-/384-well-plate), two blank reagent packs, a Users Guide and an applications notebook. Additional reagents must be purchased by the user.
Additional Materials Required
Computer
The user will need a computer for post sarnple process data analysis for single color fluorescence scans (Microsoft Internet Explorer 4.01 or higher or Netscape 4.74 or higher are required, however a Netscape 6.x and 7.x will not work with the NanoChip System), This computer should be an IBMPC or compatible, contain Windows 9x/NT/2000 ,and have a CPU speed of at least 166 mHz. A printer (preferrably color) is also required to print hand-copies of results.
96-well and 384-well Plates
The user will need to order 96-well and 381-well plates that fit into the Loader.
Notes: The plates recommended by Nanogen provide the tishtest fit for the Loader and are made of polypropylene.
The NanoChip System is calibrated for the 96-well and 384-well NuncTm plates and the MicroAmp9 Optical 96-well amplification plate (Build 4 (and higher) Loaders only). Using other plates may result in improper functioning.
Oligonucleotides/Buffers/Reagents
The user will need to provide oligonucleotides, buffers and reagents specific to the user's application. This includes sample purification kits, amplification reagents, oligonucleotides and assay specific buffers.
Instrument Specifications
NanoChip Reeder (Array Processor & Scanner)
Physical
Height 0.61 m Depth 0.69 m
Width 0.70 m Weight 66.2 kg
Electrical Supply
Line Voltage Power 100-120 VAC/220-240 VAC; 10/6 Amps
Line Frequency 50/60 Hz
Fluctuation Main supply voltage not to exceed
±-10% of nominal supply
Class 1 (grounded)
Capacity
Cartridge Capacity 1 NanoChip® cartridge
Assay Conditions
Hybridization Electronically-accelerated
Stringency Thermal, chemical and/or electronic
Sample Format
Sample carrier NanoChipa cartridge
Sample attached
to array Biotinylated 35-1000 bp nucleic acid fragments
Sample for hybridization 10-1000 bp nucleic acici fragments (<250 optimal)
Laser Information
Laser I (red laser): Excitation Wavelength 635 nm
Laser 1I (green laser) Excitation Wavelength 532 nm
Class I Laser Product
Detection Specifications
Detector Photomultiplier Tube
Emiss!on Wavelength I: 660-720 nm
Emission Wavelength II: 550-600 nm
Environmental Conditions
Temperature 18-30°C
Hurnidity 40-75% noncondensing
Pollution degree 2
Installation category II
Altitude up to 2000 m
Control Processor/Storage
Controls up to 5 Loaders
Data back up to Zip disk
Data access and analysis from external PC via web browser
NanoChip Loader (Electronic Arrayer)
Physical
Height 0.30 m Depth 0.57 m
Width 0.69 m Weight 51.0 kg
Electrical Supply
Line Voltage Power
100-120 VAC/220-240 VAC; 6/4 Amps
Line Frequency
50/60 Hz
Fluctuation Main supply voltage not exceed -±10%
of nominal supply
Class 1 (grounded)
Capacity
Cartridge Capacity 1-4 NanoChip Cartridges
Assay Conditions
Array addressing method Electronically-driven
Hybridization Electronically-accelerated
Stringency Chemical
Sample Format
Sample carrier 96-well or 381-well plate to NanoChip cartridge
Sample attached to array Biotinylated 35-1000 bp nucleic acid fragments
Sample for hybridization 10-1000 bp nucleic acid fragments (<250 optimal)
Environmental Conditions
Ternperature 18-30°C
Humidity 40-75% noncondesning
Pollution degree 2
Instalation categroy II
Altitude up to 2000m
NanoChip Cartridge
Chip Size
0.7 cm square
Array Dimensions
2 mm square
Array Site Size
80 microns
Distance between Site Centers
200 microns
Number of Test Sites
100
Permeation Layer
Thin, fully synthetic hydrogel permeation layer incorporating streptavidin
Binding Chemistry for Customizing Arrays
Electronically-driven streptavidin/biotin binding
Maximum Loading Density
Approximately 10 9 fragments per site
Minimum Sample Volume
60 µL
Storage Conditions
2-8°C
Data Analysis Computer Requirements
PC
IBM-compatible Pentium class
RAM
64M3 minimum
Browser Software Requirement (one of the following):
Microsoft Internet Explorer 4.01 or higher
Netscape Communicator 4.74 or higher (excluding versin 6.x and 7.x)
Free Hard-Drive Disk Space
10 MB minimum
Operating System
Microsoft Windows 9xNT/2000
Other Requirements
Ethernet port
PC-compatible printer