manufacturer :       Merck-Hitachi

model :                   HPLC

annotation :           Dokumente engl.

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L-7100 HPLC-Pump

Precision and Reliability

The L-7100 has been designed along the lines of the proven serial dual­piston principle, characterised by an extremely constant flow rate, low pulsation and reliable delivery of all commonly used solvents. Moreover, the L-7100 is able to form solvent gradients with high precision and accuracy. Each individual component and the finished instrument are subject to stringent quality control measures. A validation certificate and the manufacturer's test report docu­ments that all functions and specifi­cations have been fulfilled.

Versatile in Application

User-friendly programming via key­board and LCD dialogue enable the L-7100 to be used as

n Isocratic HPLC pump

n Quaternary low-pressure gradient pump in conjunction with a gra­dient accessory

n High-pressure gradient pump in conjunction with one or two further L-7100 pumps and as

n Column packing device at con­stant pressure.

The L-7100 pump can store

n 9 time programmes, in which mobile phase composition, flow rate and four events can be pro­grammed and varied over a period of time.

n Via a Link function, programmes for multi-method applications can be linked.

A shut-down routine can be pro­grammed to be carried out at the end of an analysis series by

n Delayed pump stop and a

n System flush programme.

Due to minimised internal volume, a highly constant flow-rate and excel­lent gradient reproducibility at low flow-rates, the L-7100 pump is

n Optimally suited for narrow-bore

applications.

For applications involving sensitive materials requiring operation at low temperatures

n The cold room compatibility of the L-7100 is advantageous.

Premature wear caused by salt­containing eluents is prevented by the fitting of a

n Piston cleaning facility, whereby the piston wash procedure can be carried out automatically in combination with the LaChrom autosamplers.

GLP Functions

In view of the requirements of qua­lity assurance systems, all modules of the LaChrom system have been fitted with comprehensive facilities for status display, reliability monito­ring and the provision of correspon­ding documentation and traceability.

n All parameters, programmes and gradient curves of the L-7100 pump can be printed out by the La Chrom data systems.

n The pressure signal of the

L-7100 pump is constantly moni­tored as part of the reliability check. Any deviations from the normal pressure profile, caused for example by air bubbles or malfunction of pump valves, are registered during the course of the analysis.

n The Pump pressure profile can be printed out in the analysis report and hence serve in the assessment of the quality of the analytical results.

Maintenance is facilitated by the

n Maintenance logbook which auto­matically registers the total volume delivered. This provides an indica­tion as to when the next replace­ment of the pump seal or the next cleaning of the pump valves

should take place.

Controller Function

In stand-alone operation, the system control function of the

L-7100 enables external instruments to be controlled by providing

n Four event and other control signals.

Technical Data L-7100

Delivery system                 Series configured double piston pump with electronic compensation of solvent compressibility and elimination of residual pulsation

Maximum pressure            400 bar

Flow rate                            0.001 –9.999 ml/min

Flow stability                      ≤ 0,2% RSD

Gradient modes                 Quaternary low-pressure gradient, binary or ternary high-pressure gradient

Special functions               Programme link, column pack programme, delayed pump stop,

                                          system flush programme

GLP functions                    Pump logbook, display of pressure profile deviations, key-board lock

Documentation                  Print-out of pump parameters, gradient programmes, gradient curves, pump logbook via LaChrom data systems

Outputs                              4 time-progammable event contacts, analogue pressure signal,

                                          busy/ready , D-line

I nputs                                 Start, stop, pump on, D-line

Materials                             Stainless steel, ruby, sapphire, ärconium oxide-ceramic, PTFE, carbon

L-7400 Programmable UV-Detektor

The detectors is equipped with a high-quality reference beam system which compensates for fluctuations in the light beam instability and ageing of the lamp. Newly devel­oped deuterium lamps guarantee longer life and increase the already proven operational reliability of the detectors. The measuring cells are so designed that any refractive index effects brought about by alteration of chromatographic parameters are minimised. This results in the highest degree of detection sensitivity throughout the entire measuring range of the detectors.

Wavelength Programming and Spectral Recording

Detection at the absorption maxi­mum of an analyte improves not only the detection sensitivity but also the linearity and reproducibility of an analysis. The wavelength-pro­grammable detectors are capable of adjusting automatically to the opti­mal wavelength prior to each peak. This occurs so quickly that each component of a sample is detected at its maximum sensitivity. During wavelength switching, the autozero function can be activated; this pre­vents baseline steps occurring and increases reliability when integrating peaks. In order to enable a particu­lar sample component to be specifi­cally identified, a complete spectrum can be stored and printed out on a recorder or integrator when the chromatogram has been completed.

High Operational Reliability and Ease of Use

For GLP work, information on lamp energy, lamp light-on time and an automatic test of wavelength accu­racy can be recalled. The easy-to­read display and simple operation enables the detectors to be opti­mally set. Numerous programming possibilities are available for wave­length, baseline parameters and spectral files. A total of nine time programmes can be entered using a dialogue process. Once entered, programmes remain intact even after the instrument has been swit­ched off.

Digital Communication and System Advantages

The new LaChrom detectors have been fitted with an analogue and a digital data output. They can be used either as stand-alone instru­ments or be incorporated into the LaChrom system. When combined with other LaChrom modules, digi­tal data communication ensures maximum data security and inter­ference-free data processing.

The new high-performance data communication network D-Line also facilitates the intelligent and interference-free transfer of digital information between the LaChrom modules as well as the control and display of all parameters, files and spectra via an integrator or PC. Functions such as the "event logbook", the "reliability report" and the automatically compiled service report facilitate GLP work and simplify both maintenance and checking of the instruments.

Technical Data

Optics                                              Double beam system

Light source                                     Deuterium lamp

UV cell path length                           10 mm

Wavelength range                            190–600 nm

Wavelength accuracy                     ± 1 nm

Spectral band width                         10 nm

Time constants                                Selectable, 0.1 s (FAST), 0.5 s (MED), 2.0 s (SLOW)

Noise                                                ≤ 0.8 x 10 ^–5 AU (250 nm)

Drift                                                  ≤ 3 x 10 ^–4 AU/h (250 nm)

Detector range                                 0.001 to 2.5 AUFS

Time-programmable parameters    Wavelength, auto zero, hold for baseline

Working parameters                        Time constants, recorder range, offset

Digital communication                     D-Line

Analogue output                               Data processor 1 V/AU, recorder 10 mV/ AU

Inputs                                               Marker in, spectrum start in, start in,

                                                        lamp off in, autozero in

Diagnostic functions                        Lamp energy, lamp light-on time, lamp switching, wavelength accuracy

Optional accessories                       Semi-micro, micro and preparative flow cell