manufacturer:       LTF

model:                   Clean Spot

annotation:           Dokumente engl.

PCR-Workstation:

Greater control of PCR contamination

DNA sequences often contaminate reagents, buffers, equipment, tubes and tips commonly used for PCR reactions, resulting in needless lost time and expense.

For years it has been known that UV light can damage DNA. UV exposure of 30 minutes or less in the CleanSpot can prevent unwanted amplification of contaminant DNA. The combination of unique reflectors and powerful overhead UV lights introduces pyrimidine dimers and other photo damage into the contaminating target sequences, making them non-amplifiable. The amplification process produces large quantities of DNA that are handled at various analysis stations, and event laboratories using extreme care in handling the samples will have the product DNA permeate through the Iab.

One copy of this product DNA or other extraneous DNA is an experimental reaction mixture can be amplified, leading to false positives or erroneous results. This contaminant DNA is typically introduced during reaction set up, being carried into the mixture by:

• contaminated tubes, tips, pipettes
• airborne particles laden with DNA
• fouled reaction components

The Clean Spot is designed to combat the three mayor contaminant pathways. Reaction

preparation and equipment(supply storage is performed in the "clean", enclosed, still air chamber, thus minimizing airborne and supply-borne contamination. During reaction preparation, part of the reaction mix is exposed to UV light, thus decontaminating any fouled reaction components. Since this exposure bathes the chamber and its contents, they are "cleaned" at the same time. By keeping the doors latched, the enclosed chamber and supplies are ready for the next use.

• more effective irradiation of reaction mixtures, reagents and supplies
• multiple intern irradiation
• eliminates falsely primed products in 30 minutes or less
• reduced operator exposure to UV
• white light illumination
• 32P workstation, ideal for cycle sequencing preparation isolating environment
• easy to install and clean
• advantages over other methods