Firma:             Appl.Biosystems

Modell:           7900 HT

Applied Biosystems 7900HT Fast Real-Time PCR System

•           Interchangeable blocks provide improved throughput and flexibility

•           Fast Real-Time PCR System deliversvers 96-well results in about 35 minutes

•           Custom Automation Accessory pro­vides 24-hour unattended operation

•           Powerful, flexible Standard and Enterprise Edition Software Packages provides tools for high­throughput data analysis

•           Enterprise Edition Software assists with 21 CFR Part 11 requirements

•           Hand-held and integrated bar code readers simplify sample tracking

•           Continuous wavelength detection from 500-660 nm allows the use of multiple fluorophores in a single reaction

lntroduction

The Applied Biosystems 7900HT Fast Real-Time PCR System is a high­throughput real-time PCR system that detects and quantitates nucleic acid sequences. This flexible system offers multiple block configurations, software choices, and an optional Automation Accessory, all designed to deliver the throughput and features you need. As your research expands, your system does, too. Key applications include gene expression quantitation and the detection of single nucleotide polymor­phisms (SNPs) using the fluorogenic 5" nuclease assay.

Fluorescence Detection

To induce fluorescence, the 7900HT System distributes light from an argon­ion laser excitation source to all sample wells. lt then directs the resulting (fluorescent emission through a spec­trograph to a charge-coupled device (CCD) camera. Emission wavelengths from 500-660 nm are monitored allowing the simultaneous detection of multiple fIuorophores.

System Components

7900HT Fast Real-Time PCR System

•           Peltier-based thermal cycling system with interchangeable block capability

•           Extended-life 488 nm argon-ion laser excitation source

•           Excitation light distributed to all wells via a dual-axis synchronous scanning head

•           Fluorescence detection via a spectro­graph and cooled CCD camera

•           Robot-compatible plate loading and unloading (Automation Accessory required to Ioad multiple plates without user intervention)

User-Interchangeable Blocks

The 7900HT system offers you a choice of blocks to meet your research needs:

•           lndustry standard 96-Well Block

•           lndustry standard 384-Well Block

•           TaqMan Low Density Array Block allows you to run TaqMan Low Density Arrays

•           Fast 96-Well Block delivers 96-well results in about 35 minutes

Automation Accessory

A Custom Zymark Twister Robot with five plate-stacking positions provides automatic plate-loading capability.

Each of the four input stacking posi­tions holds up to twenty-one 384-well plates or nine standard 96-well plates. The fifth position acts as an output stack.

Automation Accessory .performance plate handling errors minimizes at a frequency of less than 1% of total plates handled.

Sequence Detection Software

Both the Standard Edition and Enterprise Edition Sequence Detection System Software run on Windows XP operating system and are used for instrument control, data collection and analysis.

The upgrade to the Enterprise Edition Software includes access to two new analysis packages designed for high­throughput SNP Genotyping (SNP Manager) and Gene Expression (RQ Manager) as well as secure, robust and scalable data management in an Oracle database. This software also includes a full suite of features that comply with 21 CFR Part 11 guide­lines including data integrity, security auditing functions, electronic signatures, and electronic record-keeping.

Computer

Applied Biosystems supplies a Dell computer for use with the 7900HT system.

Installation Specifications

Using the TaqMan RNase P 384-Well or 96-Well Instrument \/erification Plate, the Applied Biosystems 7900HT Fast Real Time PCR System can dis­tinguish between 5,000 and 10,000 template copies with a 99.7% confidence level.

Assay Chemistry

Rapid assay development guide­lines are provided to ensure success when using the fluorogenic 5" nuclease assay and SYBR Green 1 dye double-stranded DNA binding assay.

Default primer and probe concen­trations are valid for multicolor SNP assays using TaqMan MGB probes, and single color quantitation assays using TaqMan probes or the SYBR Green 1 double-standard DNA binding dye assay. Assay optimization is recommended for multiplex quantitation assays to minimize PCR competition.

Reagents and Disposables

A complete line of reagents and consumables is available for use with the Applied Biosystems 7900 HT Real-Time PCR System.

TaqMan Genomic Assays

Applied Biosystems provides pre­formulated, ready-to-use, quality-tested, 5" nuclease assays for use with the 7900 HT system (see table below).The TaqMan Low Density Array is also available for use on the 7900HT sys­tem and is used to analyze tens to hundreds of targets with multiple sam­ples. lt contains user-selected, invento­ried TaqMan Gene Expression Arrays loaded and dried-down into a 384 micro-well format.

TaqMan Genomic Assays

Demonstrated Performance

7900 HT System Throughput

Block Applications

Instrument Dimensions