Firma:              J+M

Modell:                         EA 101

Kommentar:     Dokumente engl.

Using the column-switching technique EA 101 capillary electrophoresis analyzer enables to carry out the following electrophoretic techniques:

• One-dimensional capillary isotachophoresis (1D-ITP, ITP);

• Two-dimensional capillary isotachophoresis (2D-ITP);

• Capillary zone electrophoresis (CZE) with isotachophoretic sample pretreatment (ITP-CZE).

A hydrodynamically closed separation compartment of the EA 101 requires that electroosmosis is suppressed. This is achieved via the use of suitable (anticonvective) additives in the electrolyte solutions. Polyvinylalcohol, water soluble derivatives of cellulose, high-molecular weight derivatives of polyethyleneglycol, were already proved to be effective for this purpose.

Dimensions of the capillary tubes favouring high sample load capacities (1-100 µl sample injection volumes) are used to enhance the detectabilities in terms of the concentration limits of detection. Two conductivity detectors and a UV absorbance photometric detector provide means for the detection and quantitation of ionogenic analytes present in various matrices. Organic acids and bases, inorganic cations and anions, nucleosides and nucleotides, amino acids, peptides, proteins and synthetic ionogenic polymers are the groups of analytes to be mentioned in this respect.

The EA 101 consists of the following main subunits:

•     Separation unit

•    High voltage power supply with high-voltage (column-switching) relay

•    Two conductivity detectors

•    UV absorbance photometric detector

•     Control unit.

The subunits are interconnected as shown in Fig. 1 .

The EA 101 runs under ITP-WIN software delivered with the instrument.

Fig.1. A block diagram of the EA 101 column-coupling capillary electrophoretic analyzer.

HV POWER SUPPLY =         high-voltage power supply;

CD 1 =                                    conductivity detector for the preseparation (first) column;

CD2 =                                     conductivity detector for the analytical (second) column;

UV =                                       UV-absorbance photometric detector for the analytical column;

CONTROL UNIT =                 system controller;

PC =                                       computer;

HIGH-VOLTAGE RELAY =     high-voltage (column-switching) relay.