manufacturer :       HTA

model :                   HT800L

annotation :           Dokumente engl .

HT800L

The HPLC sampler

HT800L is a compact and versatile autosampler for highly precise injections into HPLC and UHPLC

Key Features:

• Large autonomy: up to 120 vial per run or 2 SBS plates
• Management of full loop, partial loop and microliter pick-up injections with unattended results
• Dilution, derivatization , phIspiking and internal standard addition capability within standard version
• Up to 10+1 reagents available
• Service friendly design

Fit most common HPLC, UHPLC and LC/MS systems . The repeatability, the versatility, the high sample throughput and the economic pricing makes this autosampier a good choice for a large variety of HPLC applications

Easy-to-use. User-friendly PC Interface to create methods and sequences in a simple way. Additional keypad designed for every operation that will be uncomfortable to do by a Data System; for wake-up and shut down maintenance, extra washing and line rising.

HT800L provides excellent analysis efficiency through its accurate syringe system that takes care to assure high performance with every vial/plate configuration unlike pressure based system.

Designed for minimal carry-over . Double step washing (by system liquid and by an aggressive solvent), internal and external needle washing and total line washing ensure a minimal carry-over.

It can handle up to 120 vials per run and it can work with different type of SBS plates. Removable rack assures easy and quick sample loading; polarization on racks assures error free procedure.

Fast . HT800L can work at very high speeds (low cycle time).

Safe . A sampling chamber sensor ensures that when the door is open a deboosting protection is activated, while lights inside sampling chamber assure an easy visual contact with samples.

Dilution, derivatization , pH spiking and internal standard addition are available within the standard version: no extra cost required. These operations can be performed in place or in an empty vial by sample dispatching ( Quantif ed Preparative).

Versatility . Up to 10+1 different reagents are available to assure complex task and long autonomy.

Different injection modality . Full loop is performed with very high accuracy values, while partial loop and microliter pick up also allow sample saving. The variable f ll speed and the viscosity delay allow a wide range of sample viscosities; programmable sampling depth assure high sample recover capacity.

Biocompatible Version

For handling more complex sample and assure a wider pH range covering.

Cooling Version

Cooling and heating capability to maintain samples and reagent at a controlled temperature.

UHPLC Version

For micro volume as low as 1ml, for pressure as high as 15,000psi.

HT800L specifcations

General specifications

Syringe volume:         500µl (Standard); Optional: 250µl and others

Loop :                            100µl (Standard); Optional: 20µl and others

Tray capacity:             120x2ml vial; micro-inserts supported

Optional:

- 2x96-SBS-plate (micro, shallow, deep)

- 2x96-PCR-plate (not-skirted, semi-skirted, skirted)

- 2x96 microtubes rack

Cycle time:                  50sec.

Valve:                           6-port, 2-position

Analytical performance

RSD (vol. >5µl):         Full loop: <0.3% ( tipically 0.15%)

Partial loop: <0.5% ( tipically 0.25%)

Carry over:                   0 (Not detectable)*

(*)Tested with Uracile 50ppm in mobile phase 75% methanol : 25% water; Column C18; 2 purge cycles; detector: UV ~ xed length

Linearity:                      0.999

Wash (Pre & Post Injection)

Washing cycle:          up to 9 (Mode: every injection/sample/step) Reagents:       

up to 2 different reagents for wash function

Filling

Sample volume:         step of 1µl

Filling speed:               programmable up to 2500µl/sec

Viscosity delay:          0-25s

Injection

injection speed:          1-2500µl/sec

Injection mode:           full loop, partial loop, microliter pick up

Air gap:                        programmable

Flush volume:             step of 1µl

Analysis time:             0-999min

Diluition , derivatization ,pH spiking, IS addiction

Sample dispatch:        step of 1µl

Reagent addiction:      step of 1µl; up to 3 different reagents

Mixing cycle:                programmable

Intermediate

wash cycle:                 up to 9 (with reagent or sample)

Reaction time:                         0-100 minutes (0.1 minutes step)

Phisical characteristics

Dimensions

( WxHxD ):                     300x346x546mm

Weight:                        ca. 19Kg

Power:                                     115V/230V; 50Hz/60Hz

Connection:                 RS232 (Standard); Optional: USB

UHPLC version

Syringe volume:          100µl (Injection mode: full loop,

partial loop)

Loop :                            5µl

RSD (vol. ≤5µl):           Full loop: <0.4%; Partial loop: <1%

Cooling

Temperature range

conditioning :                from 4°C to 60°C

Temperature

accuracy

( between the vials):     +/-1°C ( tipically +/-0,5°C)

Cooling capacity:         about 15°C below the RT (on the vial)

Working ambient

temperature :               25°C +/-10°C

Weight:                        ca. 5Kg

Require a PC for method and sequence editing.