Firma:              Cellomics

Modell:            Array Scan II-C

Kommentar:   Dokumente engl.

The ArrayScan II System is the first automated High Content Sereening (HCS) platfonn designed for multicolor fluorescence-based screens in standard microplate formats using whole cells that are analyzed individually or on a population basis. The ArrayScan II System is based on wide field epifluorescence cell imaging using fluorescence-based reagents incorporated into and/or on the surface of cells. The system incorporates automated cell identification, focusing, measurement, and analysis capabilities for multiple cellular targets and processes occurring over space and time. Multiple fluorescence markers are detected by fully automated successive recording of each fluorophore with optimized optical filter sets to achieve efficient color separation. Incorporation of a pre-aligned Mercury-Xenon lamp with a 1500 hour lifetime bulb decreases instrument down-time and enables "around-the clock" functionality.

The system also includes application software that affords immediate and powerful screening capabilities, with the flexibility to customize assays easily and quickly. Each biological application comprises a collection of optimized algorithms that automate the extraction and quantification of assay-specific biological information from cell images collected on the ArrayScan II System.

Technical Specifications

ArrayScan II Instrument

Input Voltage/Frequency         100-115VAC, 50-60 Hz –or-

                                               220-240VAC, 50 Hz

Power Consumption               6A max at 110-115V

                                               3A max at 220-240V

Computer Interface                 RS-232 serial (double shielded)

Microplates                              Most clear bottom plates are compatible

Plate Scan Region                  Rectangular area of plate, including all user defined well(s).

Stage Scan Region                 Up to a 120 mm by 100 mm rectangular area are can be   scanned with an XY resolution of 0.2 microns, and repeatability                                                of 1-2 microns.

Focus                                      Automatic for each image.

Focus Resolution                    Automatically adjusted for selected objective lens. Smallest          focus increment is 0.1 microns.

Illumination                              Mercury-Xenon illumination unit.

Wavelength Range                 350-700 nm

Filter Changers                       Automated 6 position excitation filter wheel. Manual 4 position       dichroic filter /emission filter slider.

Standard Filter Sets                Four channel fluorescence filter set with excitation at [center-        wavelength (full width at half maximum)] 365(50), 485(15),                                  546(10) and 655(30) and emission at 460(15), 520(20) and           603(30) and 710(20) for Hoechst, Fluorescein, Rhodamine and                      Cy5 fluorophores respectively.

                                               Two channel fluorescence filter set with excitation at [center-         wavelength (full width at half maximum)] 475(40) and emission                                 535(45) for Hoechst and GFP fluorophores respectively.

Max# of Channels                   6 (in a single protocol)

Camera                                   High-resolution, thermoelectrically cooled, digital camera with       12-bit dynamic range.

Optics                                      5x, 10x, and 20x objective lenses

Fuses                                      Voltage            Specifications                                     Quantity

                                               100-115 V        3AG, 250V, 8A, Fast Acting Type                 2

                                               220-240 V        3AG, 250V, 4A, Fast Acting Type                 2

Options

Additional Optics                     Optional objective magnification of 40x is available.

Additional Filters                      Filter sets are available for most fluorophores.

Robotic Plate Stacker

General

Power Requirements              2A max current

Fuses                                      Voltage             Specifications                                        Quantity

                                               120V                 3AG, 250V, 1.5A, Fast Acting Type              1

                                               240V                 5x20 mm, 250V, 0.8A, Fast Acting Type      2

Plate Capacity                         19-20 plates depending on plate manufacturer.

                                               Optional High Capacity Stacker holds up to 80 plates.

Microplates Supported            96-well and 384-well standard microplates

Handling Format                      Portrait

Plate Storage Areas                2 removable stacks (extra stacks are available)

                                               5 removable stacks for High Capacity Stacker.

Spill Control                             Interior components protected from accidental spillage by spill      shield rim located beneath rotating arm base

Weight                                     32lbs. (14.53 kg)

                                               High Capacity Stacker: 57 lbs. (25.85 kg)

Home Position                         Optical sensors for rotary and vertical arm home positions

Plate Sensor                           Mechanical pushbutton sensor

ArrayScan II System Lamp Unit

Input                                         90-115VAC/180-250VAC: automatic setting

Voltage/Frequency

                                               47-63 Hz, single phase

Current                                    8 ± 0.5A

Fuses                                      Voltage                           Specifications                          Quantity

                                               90-115V/180-250 V        250V, 5A, Delay Type (T)               1

Lamp Module                           Mercury Xenon Lamp

                                               Power Consumption approx. 200W

Lamp Module Life                    1500 hours (typical)

UV Visible Output                    350-700nm

Warm Up                                 7 minutes (typical)

ArrayScan II System Computer

Computer                                Pentium III Workstation, Ethemet 10/100 compatible

Operating System                   Microsoft Windows NT 4.0, Service Pack 5

Memory                                   512MB RAM

Hard Disk                                Two drives: (1) 9 GB and (1) 36GB for user data storage

Monitor                                     17" high resolution monitor

Printer                                      Color inkjet printer

Drives                                      CD read-write drive

Modem                                    56K external modem

ArrayScan II System

Environmental

Installation category                Class II

Pollution Degree                      Class II

Maximum Sound Pressure     <80dB

Level

Operating Temperature          59° to 104°F (15° to 40°C)

Operating Humidity                 0 to 85%, non-condensing

Storage Temperature              41° to 149°F (5° to 65°C)

Physical

Size (H x W x D)                     Space Required for Entire System:   32 in. x 60 in. x 40 in. (.8mx1.5mx1.0m)

Weight                                     Approx. 354lb. (161 kg) including weight of Stacker

Electrical

Grounding                                Through the power cord