Firma:              Byk-Santec

Modell:            LIA-mat 300

Kommentar:   Dokumente engl.

Use or Function

The Iuminescence analyzing system is a fully automatic open analyzer for the in-vitro diagnostics.

Its advantages are:

•            Module construction with automatic rack transport from the pipetting station to the measuring unit.

•            Capacity to handle up to 280 samples per loading.

•            Automatic pipetting of all samples using up to 4 assays one after the other.

•            Automatic incubation of all samples (vibrating at room temperature).

•            Automatic washing of all samples in blocks of 10.

•            Open system, suitable for supplier's standard kits: LIA, ILMA, SPALT, using coated beads or coated tubes.

•            Highly sensitive photomultipliers for measuring the luminescence reaction by photon counting.

•            The LIA-MAT SYSTEM 300 system uses transport racks and can hold 10 test tubes measuring 12 x 75 mm.

In the luminometer system two starter reagents or analyzer reagents are automati­cally added which set off the Iight reaction. Then the samples are measured and the reagents are aspirated.

The AT compatible processor can process a variety of measuring and control proce­dures. Additionally, each module contains its own intelligence, that means a micro­processor which can independently process the presented task (multi-tasking).

A standard curve can be calculated from the measurement results or the standard sera of the immunoassays.

The unknown concentrations of the patients sera/control can be calculated from this curve.

Principle of Luminescence

The luminescence is a reaction during which light (photons) is emitted.

A molecule is stimulated by absorbing chemical energy. From this stimulated condi­tion the molecule goes back to a lower level by emitting Iight of a defined wave length.

We distinguish between bioluminescence and chemiluminescence:

Bioluminescence are the light phenomena of living organisms Ilke the glow-worm, the American firefly or photo bacteria. By oxidation the production of Iight is catalyzed by specific enzyme systems.

The chemiluminescence has the same physical basis, but no living organisms are in­volved. The Iight-producing molecules are chemical compounds which can be acti­vated by oxidation. They can release their stored energy within shortest intervals of time in a light-emitting reaction. The Iight reaction is not spontaneous but can be set off at a specified point of time by injecting a starter reagent.

Before this injection takes place, a catalyst or an acid has to be added.

The emitted photons hit the multiplier and are there integrated (counted) during the measurement time. The unit of the light mass is RLU (relative light units).

The most frequently used luminogeneuous compounds are:

-            acridinium ester I salts

-            aminophthal hydrazide

The luminescence immunoassays are non-isotopical immunochemical processes for the laboratory diagnostic whose antibodies and antigenes are Iabeled luminogeneu­ously.

The Iuminescence assays are in their sensitivity and specification comparable with radioactive assays, but oller the advantages in handling and disposal since they are not labeled radioactivity.

Similar to the radioactive assays, different types of assays are distinguished:

LIA        Iuminescence immunoassay

ILMA     immunoluminometric assay

SPALT             solid phase antigen Iuminescence technique

Depending whether it is a competitive or a sandwich assay, the results are falling or rising standard curves.

In any case, the Iight mass is proportional to the amount of luminogeneuous sub­stance (tracer) in or at the test tube.

The released Iight mass therefore correlates with the concentration of the tested analytes.

TECHNICAL DATA

Sample capacity per loading   max. 280 samples an 28 racks

                                               10 samples/rack

Test tubes                               Ø 12 mm x 75 mm high

Rack dimensions                    164 x 18 x 46 mm (LxWxH)

LUMINOMETER

Test method                                       Luminescence measurement according to the pho. count method

Photomultiplier                        Bialkali, 300 - 650 nm, linearity 5 decades

Measurement time                  0.2 - 99.9 sec in tenth of seconds

Injection                                   2 starter reagents with 300 µl each.

                                               Automatic aspiration of reagents

Volume accuracy                    (with 300 µl)     < 2%

Volume precision                    (with 300 µl)     < 1%

Reagent container                   2 square PE containers 250 ml

                                               1 glass aspiration container 500 ml

Processor                               AT compatible, VGA,

                                               3.5" floppy (1.44 MB), 40 MB hard disk

WASHER/INCUBATOR

Washer                                   10 samples simultaneously

                                               Wash cycle programmable

Volume accuracy                    (at 4 ml) < 10%

Volume precision                    (at 4 ml) < 2%

Incubator                                 280 samples simultaneously Incubation time programmable Circular vibration frequency 7 Hz.

Thermo Incubator                               Cooling/heating to 21/37° Celsius Temperature preciseness: +1- 2°

Cooling speed: 10° 145 minutes

Heating speed: 10° / 35 minutes

PIPETTING STATION

Pipetting station                       Programmahle

                                               max. 300 secondary samples

                                               max. 12 standards / 6 controls per assay

Volume accuracy (1.5 ml)       (at 100 µI)        < 1%

                                               (at 10 µI)          < 5%

Volume precision (1.5 ml)       (at 100 µI)        < 0.3%

                                               (at 10 µl)          < 1.5%

DIMENSIONS:

Luminometer                           625 x 880 x 770 mm, 48 kg

Washer/Incubator                   480 x 615 x 800 mm, 42 kg

Pipetting station                       775 x 600 x 710 mm, 50 kg

ACCESSORIES

Monitor                                     14" (inch), VGA, radiation-free

Printer                                      Graphic printer (proprinter II - comp)

Compressor                            quiet, max. 8 bar

OPERATING CONDITIONS

Operating temperature            15 - 35 °C        (@ relative humidity 20 - 80 %)

Storage temperature               0 - 60 °C          (@ relative humidity 10 - 80 %)

SOFTWARE

LIAMATIC                                 max. 100 different assay reports

                                               max. 999 patients

                                               max. 12 standard sera

max. 6 controls max.

10 replicates

EVA-PACKAGE                      Pipetting method setup

Pipetting station direct drive

Test programs

ELECTRICAL DATA (voltage, amperage, frequency)

Luminometer / Monitor            110 V (+/-10%) / 3.2 A/60 Hz

Washer / Incubator                 110 V (+/-10%)/ 0.7 A / 60 Hz

Thermo Washer / Incubator   110 V (+/-10%) / 4.0 A / 60 Hz

Pipetting Station                      110 V (+/-10%) / 4.0 A/60 Hz

Printer                                      110 V (+1-10%) / 1.0 A / 60 Hz

Compressor                            110 V (+/-10%) / 32.0 A / 60 Hz

Fuses (for replacment)      

Luminometer                           5.0 A

Monitor                                     2.8 A

Washer / Incubator                 0.8 A

Thermo Washer / Incubator    4.0 A

Pipetting Station                      4.0 A

Printer                                     1.0 A