Firma:              BioRad

Modell:            GS-525

Kommentar:    Dokumente engl.

The Molecular Imager Systems

The GS-525 and GS-505 Molecular Imager systems form a versatile system for capturing digital images of radioactive and chemiluminescent samples using a storage phosphor technology. The systems eliminate the need for x-ray film, as well as a darkroom and developing chemicals, by capturing an image on a reusable Imaging Screen. This technology is more accurate and more sensitive than x-ray film autoradiography. Due to the wide linear dynamic range of the Molecular Imager system, radioactive and chemiluminescent samples can be accurately quantitated over a wide range of concentrations. The expanded linear response minimizes the chances of over- or under-exposure commonly encountered with film, where an incorrect exposure affects image quality and quantitation, and requires repeated exposures. In addition, the sensitivity of the Molecular Imager system permits detection of radioisotope signals 10 to 100 times faster than film. For chemiluminescence, this system requires only seconds or minutes of exposure for most applications.

The GS-525 Molecular Imager system (Figure 1.1) consists of the five components: the storage phosphor Imaging Screen Cassettes (20 x 25 cm or 35 x 43 cm), the Laser Scanner or Molecular Imager per se , the Sample Loading Dock, the Screen Eraser, and the Molecular Analyst ^® software (Macintosh ^® or PC) which runs on your host computer. The Imaging Screen contains microscopic storage phosphor particles which are extremely sensitive to radiation in the form of both visible light and emissions from isotopic decay. Exposing a screen to such emissions creates a latent image, which is subsequently recovered by scanning the screen with the infrared laser in the Molecular Imager system The signal is recorded as a digitized image in the host computer. The GS-505 Molecular Imager system consists of all the above, and accepts only the 20 x 25 cm screens. The Molecular Analyst software processes this image on a gray scale with 216 or 65,536 levels for quantitative analysis, and for video display and hard-copy output. The Sample Loading Dock is a light-tight apparatus for exposing the Imaging Screen to your samples. The Imaging Screens are prepared for reuse in the Screen Eraser, which floods the phosphor with infrared light. This brings the storage phosphor in the entire screen back down to its base state.

Fig. 1.1. GS-525/GS-505 Molecular Imager systems.

System Technical Specifications

Linear dynamic range                  1:100,000

Pixel resolution                             100, 200, or 800 µm, selectable

Image resolution                          2.5 line pairs/mm or 200 µm

Pixel density                                 16 bit (0 - 65,635)

Signal decay                                32P 75% retention 24 hr

Scanning area                              35 x 43 cm or 20 x 25 cm

Operating Conditions:

Supply voltage                              100–240 VAC ±10%

Frequency                                    50–60 Hz

Environmental conditions:

Temperature                                0 to 70 °C

Humidity                                       0 to 95%

Total weight                                  60 kg

GS-525/GS-505 Molecular Imager Component Specifications

Laser Scanner

Dimensions                                  43 (depth) x 48 (width) x 17.5 (height) cm

Construction                                Aluminum chassis

Weight                                          31 kg

Electrical-

Maximum power                          650 Watts

Input voltage range                       100–240 VAC, 50–60 Hz

Fuses                                           No user-serviceable fuses

Environmental-

Operating                                     50°F (10°C) to 90°F (32°C) temperature

                                                    30-80% humidity

Storage                                        32°F (0°C) to 140°F (60°C) temperature

                                                    10-90% humidity

Screen Eraser, GS-525

Dimensions                                  23 (height) x 51 (width) x 57 (depth) cm

Construction                                Aluminum chassis

Weight                                          13.6 kg

Input voltage range                       100/120 VAC, 50–60 Hz

                                                    220/240 VAC, 50–60 Hz

Fuses                                           6.3 Amp (100/120 VAC)

                                                    3.15 Amp (220/240 VAC)

Illumination                                   12 x 40 Watt user-replaceable bulbs

Loading Dock, GS-525

Dimensions                                  63.5 (length) x 49.5 (width) x 11.4 (height) cm

Weight                                          14.5 kg

Construction                                Aluminum chassis

Screen Eraser, GS-505

Dimensions                                  19.7 (height) x 37.5 (width) x 47.6 (depth) cm

Construction                                Aluminum chassis

Weight                                          10 kg

Input voltage range                       100/120 VAC, 50–60 Hz

                                                    220/240 VAC, 50–60 Hz

Fuses                                           3.15 Amp (100/120 VAC)

                                                    1.6 Amp (220/240 VAC)

Illumination                                   6 x 40 Watt user-replaceable bulbs

Loading Dock, GS-505

Dimensions                                  42.7 (length) x 42.9 (width) x 10.2 (height) cm

Weight                                          7 kg

Construction                                Aluminum chassis

Imaging Screen Cassette, Large Format

Dimensions                                  63.5 (length) x 37.5 (width) x 2.3 (height) cm

Weight                                          2.25 kg

Construction                                Aluminum, laminate on honeycomb

Imaging Screen Cassette, Small Format

Dimensions                                  48.5 (length) x 37.3 (width) x 2.1 (height) cm

Weight                                          2.7 kg

Construction                                Aluminum