manufacturer:       BioRad

model:                   xCell gene pulser

annotation:           Dokumente engl .

Features and Benefits

§         Universal electroporation - transfects all cell types, from primary and stem cells to bacteria and yeast

§         Preset protocols - include the most common mammalian and bacterial cell types

§         Flexibility — choice of programs for preset protocols, optimization protocols, manual operation, and/or user protocols

§         Protocol library - collection of electroporation protocols for every cell type including primary, immortal, and bacterial cells

§         Data management — enables storage and recall of parameters used in the previous 100 experiments, useful for troubleshooting

§         Reproducibility — uses PulseTrac ™ circuitry and arc-protection features to ensure reproducibility and sample protection

Specifications Gene Pulser Xcell ™ Total System

(incl. main unit, CE module, PC module and ShockPod ™ cuvette chamber)

Outputs

Waveform:       Exponential or square

Voltage:           10-3,000 V

Capacitance

10-500 V, 25-3,275µF in 25 µF increments

500-3,000 V, 10, 25, 50 µF

Resistance (parallel)

50-1,000 Ω in 50 Ω increments, plus infinity

Sample resistance

20 Ω minimum at 10-2,500 V

600 Ω minimum at 2,500-3,000 V

Square-wave timing

10–500 V: 0.05–10 ms in 0.05 ms increments, 10–100 ms pulse in 1 ms increments, 1–10 pulses, 0.1–10 sec interval

500–3,000 V: 0.05–5 ms in 0.05 ms increments, 1–2 pulses, 5 sec minimum interval