Firma:              Becton Dickinson

Modell:            FACS Vantage

Kommentar:   Dokumente engl.

The FACS Vantage ^TM , BDIS's new cell sorter, was designed for ease of operation and provides ample room in which to work. With its stream-viewing video camera and monitor, an eight-trace oscilloscope, a wide selection of lasers, an optional third laser, FACS Vantage uses the latest technology in flow cytometry.

You can use FACS Vantage for research applications in:

Molecular Genetics to study

• isolated chromosomes for developing chromosome-specific libraries

• normal gene expression

• abnormalities leading to disease

Cell Cycle Analysis to

•     quantify the proliferative characteristics of cells and cell populations

•     gain knowledge about the fraction of cells at risk from cell-cycle—specific cytotoxic agents, enabling maximal tumor killing and minimal normal cell death

•     quantify phase fractions, phase durations, doubling times, labeling index, and growth fractions by using modeln or bromodeoxyuridine as a marker for cell­cycle traverse studies

•     employ the monoclonal antibody Ki-67, a nuclear antigen that is found in proliferating cells, but not in resting cells

•     perform leukemia phenotyping, monitor cancer remission, and analyze rare events in cancer studies

Intracellular Calcium Measurement to

•     measure intracellular ionized calcium concentration to learn about a cellular functions

•     correlate calcium concentration with other parameters, such as cell-surface antigen expression

•     sort, based an calcium concentration

Fluorescence In Situ Hybridization (FISH) to

•     label, locate, and quantify specific nucleic acid sequences in cells or chromosomes, alter sorting specific cells an FACS Vantage

•     enhance FISH techniques by adding the FACS Vantage spatial resolution, convenience, speed, and sensitivity

Large Particle Analysis and Sorting (using the available MacroSORT Option) to

•     characterize and sort islets of Langerhans for diabetes research including transplantation studies and encapsulation

•     measure nuclear genome sizes in higher plants by producing a suspension of cells without walls (protoplasts) and by measuring the DNA content

•     evaluate DNA content by studying megakaryocytes

Microbiology to

•     analyze changes in cellular functions brought about by intracellular organisms such as viruses, bacteria, and protozoa

•     study cell-cycle kinetics and regulatory mechanisms governing cell growth and replication

•     monitor (bacteria and other) microorganisms used in industrial processes or occurring as contaminants in materials ranging from drinking water to crude oil.

Optional Laser

Enterprise II Ion Laser

The Enterprise ion Laser consists of a water cooled Laser head and a switching regulated air cooled power supply. Enterprise is a small frame argon ion Laser available with multiple wavelength outputs including UV, UV/488 nm, and UV/488/515 nm outputs.

The Enterprise System can be remotely controlled by an external device via a connector an the power supply (remote operation), which accepts analog commands and provides analog Status outputs, or directly from the front panel of the power supply (local operation).

(1) Beam diameter is measured at the output coupler which is 8.5 cm behind the front faceplate of the laser head assembly.

(2) The long-radius output coupler acts as a weak lens to transform the output beam parameters. The values listed for beam divergence, waist diameter and location take into account this weak lens effect. The value for beam waist location is measured from the outside of the front faceplate of the laser head assembly.

(3) Primary wavelength(s) used for light regulation. When the UV power level is set for the specified value, the visible power levels may be significantly higher than the specified values.

(4) Other wavelengths may be present.