Applied Biosystems API 3200
Objektnummer | B00018283 |
---|---|
Seriennummer | 018283 |
Object Naam | Applied Biosystems API 3200 |
Status | Archiv |
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manufacturer : Applied Biosystems
model : API 3200
annotation : Dokumente engl.
The following illustrations and descriptions refer to the instrument model and are drawn from brochures. They do not represent the scope of delivery. Please refer to the text of the offer for the exact scope of delivery.
The API 3200 LC/MS/MS system is a fully-integrated triple quadrupole mass spectrometer built using premier technology that combines sensitivity and selectivity with ruggedness and reliability, all in an affordable, compact package
Product Description
· Innovative Turbo V ion source efficiently ionizes compounds over a wide range of flow rates, virtually eliminating cross-contamination, even with large sample loads
· Patented LINAC® collision cell technology delivers superior MS/MS performance allowing faster scan times without compromising sensitivity or mass spectral quality
· Simplifies cross-platform methods transfer
· Gives meaningful results from every experiment
· High Performance The API 3200 LC/MS/MS system incorporates a number of proprietary mass spectrometry innovations to provide the performance and reliability required by demanding applications in regulated environments. The Turbo V ion source and patented LINAC collision cell enable high-sensitivity quantitation of multi-component analyses over a wide range of flow rates. Together with the compact ion optics and pumping technology, this system ensures low limits of detection and quantitation for your lab.
· Higher Productivity Rugged Curtain Gas interface technology protects the interface region and quadrupole analyzer from contamination, reducing the need for routine maintenance. With this increase in uptime plus software that enables automated setup, analysis, and methods development, you can easily run hundreds of samples per day.
· More Value With an unmatched heritage of technology innovation and dependability, the triple quadrupole API 3200 system offers an attractive blend of performance, throughput, and value.
Optional Equipment:
Perkin Elmer Series 200 HPLC Systems
Series 200 Column Oven
Precision temperature control improves retention time
In many HPLC analyses, variations in column temperature can cause significant changes in retention times, making qualitative analysis difficult and affecting the precision of quantitative measurements.
By precisely and accurately controlling the temperature of
the mobile phase and the column, optimal results can be achieved. By maintaining the column temperature at a stable set point, more standardized results can be obtained, permitting better dayto-day and lab-to-lab comparison of results. In addition, elevated temperatures can be used in many applications to decrease mobilephase viscosity while increasing mass transfer and sample solubility, resulting in better resolution and/or increased speed of analysis. Finally, subambient temperature capabilities are helpful in many biomolecular analyses and in the analysis of some chiral compounds.
PerkinElmer offers three versions of the Series 200 Column Oven to meet your specific needs: heat-only, Peltier (heat and cool), and a Peltier unit with a built-in column selection/switching valve.
The PerkinElmer Series 200 family of column ovens controls the mobile phase and sample temperature before they enter the column via a built-in preheater. This eliminates thermal gradients across the column, resulting in better column performance and precise retention times. All heating and cooling (Peltier units only) is performed electrically, permitting subambient temperatures as low as 5 ˚C without coolant or refrigerants.
The Series 200 Oven/Selector permits automated switching of up to six HPLC columns. This feature is extremely beneficial for method development applications and in multiuser/multi-method labs.
Key Benefits
Ø All-electronic design permits wide operating range and leak alarm Peltier units do not require refrigerants or coolants
Ø Peltier oven/selector unit includes remote-control software, permitting full oven and selector-valve control in real-time
Ø Integrated solvent pre-heater/chiller minimizes temperature gradients
Ø Large, easily-accessible column compartment holds even 30-cm column formats
Ø Fully-stackable design integrates into complete Series 200 HPLC System
The Peltier oven/column-select model includes an easy-to-use software-control package. This allows full remote control of oven temperature and the columnselection valve. Temperature and column selection can be programmed within a single method or sequence of runs allowing full, unattended operation.
The Series 200 family of ovens is designed to meet even the most demanding requirements. Microprocessor-based control maintains the set temperature, with 0.2 ˚C stability, across the entire temperature range. The LED display continuously shows the current actual temperature and the user selected set temperature. The unique, easily-accessed column compartment can accommodate multiple HPLC columns including 30-cm columns such as those used in GPC analyses. The Series 200 ovens also contain an integrated leak-detection system, alerting the users to the smallest of leaks.
Stackable unit saves bench space
Because the column compartment is easily accessed directly from the front of the unit without having to remove a top cover, it can be stacked with other instruments in the Series 200 family, preserving valuable bench space.
Specifications Series 200 Column Oven
Operating Range (Heat-only units) 30 °C to 90 °C
Operating Range (Peltier units) 5 °C to 90 °C (max 15 °C below ambient)
Operating Range (Oven/col select units) 7 °C to 90 °C (max 15 °C below ambient)
Recommended Flow Range Up to 5 mL/min
Temperature Accuracy ± 1 °C over entire range*
Temperature Stability ±0.2 °C
Temperature Repeatability ± 1 °C
Heating Rate (Heat-only units) 30 min to setpoint at 2 mL/min
Heating / Cooling Rate (Peltier units) 30 min to 10 °C or 60 °C at 2 mL/min*
Temperature Ramp Up to 5 °C/min*
Safety Cutoff Leakage sensor
Stabilizing Time 30 min after reaching setpoint
Column-Selection Valve Capacity 6 switching positions
Power Requirements
Power Consumption 120 Watts
Line Voltage Range (Heat-only units) 120 or 220/240 VAC 50/60 Hz ±10%
(unit set to 220 VAC at factory)
Line Voltage Range (Peltier units) 115 or 230 VAC 50/60 Hz ±10%
(unit set to 230 VAC at factory)
Operating Conditions
Ambient Temperature 10 °C to 35 °C
Ambient Humidity 20 to 80% (without condensation)
Altitude 0 to 2000 m
Physical
Dimensions (HxWxD) 16 x 34 x 48 cm
Weight 9 Kg
Series 200 Autosampler
The PerkinElmer Series 200 LC Autosampler is an advanced sample processor that can be used with virtually every liquid chromatography system or application. You configure the Autosampler and define all of your methods and operations using function keys on a keyboard located on the front panel of the instrument. A backlit Liquid Crystal Display (LCD) located directly above the keyboard shows both status information about the instrument and provides access to the screens you use to define methods and set configuration parameters. If you use the PerkinElmer Series 200 LC Pump, you will find that the Autosampler front panel is almost identical to the one on the Pump. In addition, the Pump and the Autosampler share many common operating features that will allow you to get your system up and running quickly. The Series 200 LC Autosampler provides you with interchangeable technology so that you do not have to buy a new Autosampler and learn new user interfaces every time your needs change.
Features and Benefits
Ø Upgradable to biocompatible versions for maximum flexibility.
Ø Small, compact design (12.5 inches (31.8 cm) wide) saves valuable bench space.
Ø Directory of up to 20 methods.
Ø Ability to link up to 10 methods together in a sequence.
Ø Priority method which allows you to interrupt a method or sequence to run critical samples. The method or sequence resumes once the priority samples are analyzed.
Ø Edit the active method or sequence in real-time with easy access function keys.
Ø Large backlit liquid crystal display allows clear viewing of autosampler method and operating parameters at all times.
Ø Tactile keyboard with unique key click so that you know your entry has been made.
Ø Phone type contact closure connections and finger tight plumbing fittings make it simple to connect or disconnect your system.
Ø Full method documentation including modification with method name, number, date and time stamp.
Ø Visual access front-door panel allows quick plumbing inspection and easy servicing.
Ø Maintenance log coded by the serial number of the autosampler, including autosampler injection cycles.
Ø Instrument log records history of operation for quick diagnosis and servicing to get you up and running quickly.
Ø Extensive service diagnostics.
Ø Sampler module can be completely removed to lab bench for maintenance without having to unstack modules.
Specification Series 200 Autosampler
Injection range Programmable from 0.1 - 1.0 µL at 0.1 µL increments, 1 µL to 2.5 mL at
1 µL increments
Replicate injections 1 - 99 from one vial
Precision Typically <0.5% RSD of peak areas from 3 µL
Linerarity >0.999
Minimum sample volume 1 µL in 7 µL
Sample syringe sizes 50, 100, 250, 500, 1000, 2500 µL
Sample waste None (or user selectable)
Carryover <0.02%
Flush cycles User selectable, 1 - 9
Flush volume 0 - 2500 µL
Sample capacity up to 225 vials with optional tray
Method parameters Injection volume, sample draw speed, flush volume, flush cycle,
2 external contacts, start and stop time
Methods Battery-backed storage of up to 20 methods including time pro‑
gramming. Editing of stored methods possible during runs.
Injection cycle time 2 - 3 samples per minute
Valve materials in contact with With Rheodyne 7725 for Analytical - stainless steel, Vespel, sample ceramic, PEEK
With Rheodyne 9725 for Bio Applications - PEEK, Tefzel, ceramic
With Rheodyne 8125 for Microbore - stainless steel, Vespel, ceramic, ^ PEEK
Display 7-line by 40-character backlit liquid crystal display
Communications Integrated communications for derivatization and dilution option s
Serial communications - standard
BCD interface - standard
5 relay outputs; 2 timed events, 2 inject, 1 start
AC Power Requirements:
AC power 70 Watts
AC voltage range Grounded 100 ,120,220,230,VAC (±10%)
Grounded 240 VAC (+6%/-10%)
Frequency 50/60 Hz (±1%)
Fuse rating characteristics:
100/120 VAC Single Fuse 1.6 A 3 AG Slow Blow, 250 V
220-240 VAC Single/
Dual Fuse 0.8 A TT (5x20mm), 250 V
Operating Conditions:
Environment This instrument is desigend for indoor use. It is not designed to operate in an explosive environment.
Ambient temperature 10 -30 °C
Ambient relative humidity 10 -80% (without condensation)
Altitude 0 to 2000 meters
Installation Category This instrument is able to withstand transient overvoltage according to Installation Categroy II as defined in IEC 1010-1.
Pollution Degree This instrument will operate safely in environments that contain nonconductive foreign matter to Pollution Degree 2 in IEC 1010-1.
Peltier sample cooling
temperature range 4 50°C, ± 2°C
Series 200 Micro Pumps
Top performance characteristics are what you have come to expect from PerkinElmer ® LC pump s. The PerkinElmer Series 200 Micro Pump is a fifth-generation pump with superior flow rate precision, compositional accuracy and ex ceptional retention time repeata bility for all of your separations. The Series 200 Micro Pumps provide precise high pressure gradient capabilities for microbore, narrowbore, LC/MS and analytical applications.
Key Features
Ø Small, compact design [12 inches (30 ,5 cm) wide] saves valuable bench space.
Ø Large back-lit LCD screen allows clear viewing of pump method and status parameters at all times.
Ø Solvent resistant front panel with rounded ergonomic design for easier interaction with keypad.
Ø Total Chrom ready just connect to the link interface and go! No up-grades or additional cables required!
Ø Mass Spec Ready full control from
o Analyst (Sciex)
o Excalibur (Finnigan)
Ø Based ont the field-proven Perkin Elmer pump design, this combination of two pumps provides precise gradients and pulseless flow for microbore, narrowbore and analytical requirements with minimal delay volume.
Ø Pump module can be accessed or compeltely removed to lab bench for maintenance without having to unstack modules via unique slide out design.
Specifications Series 200 Micro Pumps
Fl ow Rate Range 1 µL/min - 3 mL/min
Flow Rate Increments 1 µL/min from 0 to 0.999 mL/min; 0.01 to 3 mL/min
Fl Precision 0.3% RSD (typical 0.1%) at 300µL/min water @ 1000 psi
Fl Accuracy ±1% of setting at 300 µL/min and 1000 psi with water
Retention Time
Reproducibility < 0.3% RSD (typically < 0.1%)
Compositional Accuracy Typically 0.5% from 10 to 90% up to 2 mL/min
Compositional Precision Typically < 0.2% variation
Gradient Forms Linear, convex and concave w/high pressure blender
Linearity Within 1% from 10 to 90%
P rogram Steps Step 0 (Equil) plus up to 20 solvent program steps. Battery-backed
storage of up to 20 methods plus 1 edit method and 1 default method
St ep Time 0 to 999 minutes
Step Time Incerements 0.1 min from 0 to 9.9 min; 1.0 min from 10 to 999 min
Timed Events Two built-in independent momentary (2 second) contact closures
Events/Method Max of 9
Event Increments 0.1 min from 0 to 9.9 min; 1.0 min from 10 to 999 min
Dimensions (H x W x D) 14 cm x 30.5 cm x 43 cm (Binary Micro Pump 30 cm height)
Weight 27.3 kg (60 lbs)