Firma:              Agilent

Modell:            G 1321 A

Kommentar:   Dokumente engl.

Agilent 1100 Series Fluorescence Detector

Optimized sensitivity and selectivity, for reliable quantitative and qualitative information

The Agilent 1100 Series fluorescence detector provides high-sensitivity multisignal detection, online fluorescence spectra, straightforward Operation and maintenance, and built-in features for GLP compliance.

•            Simplified optical design for optimized baseline stability.

•            Long-life Xenon lamp for highest sensitivity.

•            Variable-mode monochromators for selective multisignal detection and

online spectra acquisition.

•            Easy front access to flow cell for fast inspection or exchange.

Increase selectivity with multisignal detection

The monochromators work in signal or spectral mode and are synchronized with the flash lamp to allow single or multi­signal detection of up to four different excitation and emission wavelengths.

Get spectra and quantitative data from a single run

View online spectra without interrupting your chromatography. Excitation or emission spectra are acquired in just tenths of a second. Save all the data for postrun review.

Quantitative information in trace analyses

The optical design guarantees lowest stray-light performance for optimized baseline stability. A Jong-life (>4000 hours) flash lamp, lamp reference system and efficient Iight collection ensures constant lamp energy for maximum excitation of fluorophores.

Check or exchange your flow cell—fast

Plus automatic recognition of all flow cell cartridges provides documentation of instrument parameters and helps you to comply with GLP.

Specifications

Detection type:                        Multisignal fluorescence detector with rapid online scanning capabilities and spectral data analysis

Performance:                          10 fg anthracene LOD, Ex 250 nm, Em 400 nm*, Raman (H ₂ 0) >400 Ex 350 nm, Em 397 nm, dark value 450 nm, standard flow cell

Light source:                                       Xenon flash lamp, normal mode (20W) and economy mode (5W)

Pulse frequency:                     296 Hz for signal mode, 74 Hz for economy mode

Excitation monochromator:    Concave holographic grating, F/1.6, blaze 300 nm, range 200—700 nm and zero-order, bandwidth 20 nm

Emission monochromator:     Concave holographic grating, F/1.6, blaze 400 nm, range 280—900 nm and zero-order, bandwidth 20 nm

Reference system:                 Inline excitation measurement

Time programing:                    Up to 4 signals, response time, PMT gain, baseline behaviour (append, free, zero), spectral parameters

Spectral acquisition:                Excitation or emission spectra, scan speed 28 ms per datapoint, e.g. 0.6 s per spectrum 200—400 nm, 10 nm step

Step size:                                1—20 nm

Spectra storage:                     Limited only by disk space

Wavelength repeatability:        ±0.2 nm

Wavelength accuracy:            ±3 nm

Flow cells:                               Standard: 8 µl volume, 20 bar (2 MPa) pressure maximum, quartz

                                               Optional: fluorescence cuvette for offline spectroscopic measurements with 1 ml syringe, 8 µl volume, quartz

Control and data evaluation: Agilent ChemStation for HPLC or Agilent 1100 Series control module with limited spectral data analysis and printing of spectra

Analog outputs:                       Recorder/integrator: 100 mV or 1 V, output range >10 ² luminescence units, 2 outputs

Communications:                    Controller-area network (CAN), HP-IB, RS-232C, LAN, APG Remote: ready, start, stop and shut-down signals

Safety and maintenance:        Extensive diagnostics, error detection and display (through control module and ChemStation), leak detection, safe leak

            handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.

GLP features:                          Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy using Raman band (H ₂ O).

Housing:                                  All materials recyclable

Environment:                                      0 to 40 °C constant temperature at <95%, humidity (non-condensing)

Dimensions (h x w x d):          140 mm x 345 mm x 435 mm, (5.5 x 13.5 x 17 inches)

Weight:                                    11.5 kg (25.5 Ibs)

* Reference conditions: standard cell 8µl, response time 4s, 2.1 x 100 mm ODS column, 0,4 ml/min flow rate.