Firma:              Waters

Modell:            715 Ultra WISP

Kommentar:   Dokumente engl.

The Waters 715 Ultra WISPTM Sample Processor, referred to as the Ultra WISP, is a versatile, easy-to-use sample processor module that fully automates the injection process for high performance liquid chromatography (HPLC) instruinentation. The Ultra WISP may be programmed to inject up to 96 sample aliquots in either sequential or random fashion. Injection volumes are programmable from 0.1 to 2000 microliters (ul).

The Ultra WISP provides the following features:

•     Microprocessor-based all electric design

•     CRT with programming keypads

•     Patented dual port restrictor loop injector design

•     Sequential or random vial operating program

•     IEEE and RS-232 communications

•     Integrated needle wash system

•     Three syringe options

•     Two sample loop sizes

•     Two carousel sizes

In addition to programming a normal auto run, the Ultra WISP program enables you to:

•     Interrupt and then resume a normal run to perform a stat run

•     Run standards separately from the method table to simplify programming

•     Perform auto addition, co-injecting the contents of a common vial with the samples

•     Perform auto transfers to withdraw up to three reagents to transfer to samples

•     Program external event outputs

•     Suspend a run to reprogram sample parameters or to add sample vials to the carousel

•     Program the needle to withdraw sample from different levels in the vial

•     Purge the system and perform compression and bubble checks

The Ultra WISP is compatible with the Waters PowerLineTM family of HPLC systems and can also be programmed from a Waters 600E System Controller.

Maintenance of the Ultra WISP is simple due to the modular design of the flow path. The Ultra WISP is self-diagnosing, displaying system and error inessages when appropriate. For further diagnosis, you may also manually operate the individual assemblies.

General                                             The Ultra WISP can routinely operate with system    backpressure up to 4500 psi.

                                                         Any LC solvents compatible with current pump and column technology can be used with the Ultra WISP.         Salts and buffers can reduce seal life, especially at          system backpressures in excess of 3000 psi, and in          absence of needlewash and regular maintenance and                             cleaning.

Physical Requirements                    Electrical Power - Nominal input voltage 100, 120, 220,        240 at 48-52, 58-62 Hz

                                                         Voltage may vary by +10% of nominal; a variation of -           10% longer than 13 milliseconds is considered a power                                                          loss.

Sample Capacity                              48 Vial Carriage - 48 - 4 ml or optional 250-µl Low Volume   Insert (LVI) screw cap vials per carriage; Teflon self-                                                          sealing or Teflon disc septa.

                                                         96 Vial Carriage - 96 - 1 ml snap-in push cap or optional      150-µl LVI vials per carriage.

Sample Volume                                48 Vial Carriage - 2.0 ml maximum injectable volume           (from 4 ml total); liinited volume inserts (LVI) allow                                                          minimum sample waste volume of 6 µl (maximum   injectable volume - 244 µl).

                                                         96 Vial Carriage - 1 ml maximum injectable volume.             Minimum sample required - 300 µl. Limited volume inserts                                              (LVI) allow maximum injection volume of 120 µl.

Minimum Sample Volume                0.1 microliters

Functions                                          Replicate injections as programmed from the controller

                                                         Output signals to reset gradient, start integrator, flow           detector

                                                         Purge flow path with mobile phase

                                                         Discrete needlewash to prevent cross contamination

                                                         Programmable standard sequencing

Carry Over                                        Less than 0.1% previous sample component            concentration; except through sample interaction with 304                                                          stainless steel parts.

Cross Contamination                       Dependent on selection of needlewash solvent and sample interaction with 304 stainless steel parts.

Injection Volume Delivery                 Mobile phase (degassed) tested at 100 psi, with Teflon        disc vial septa, auxiliary sample loop out and preset                                                          operational parameters.

                                                         Mobile Phase                 Actual Volume (%)* at:

                                                                                               5µl             10µl      50µl

                                                         3% acetone in MeOH     95              98         99

                                                         Napthalene in CH2C12 75              85         95

                                                         3% acetone in H20        99              99         99

                                                         *Shown as a percent of programmed injection volume.

                                                         Theoretical results - actual is solvent dependent.

                                                         Reduced delivery will be observed for MeOH and      CH ₂ Cl ₂ with higher solvent compressibility self-sealing       septa and auxiliary sample loop.

                                                         Most accurate injection volume (all solvents): 50 µl.

Reproducibility                                  Less than or equal to 1 % for 1 to 100 µl injections.

Linearity                                            Coefficient of determination of greater than .99 for a range   of 0-100µl.

Ultra WISP Part number                   044500

Dimension                                        Height: 17 inches (43.2 cm)

                                                         Length: 24 inches (61.0 cm)

                                                         Width: 15.25 inches (38.7 cm)

Weight                                              94 pounds (43 kg)

Desired Site conditions                    • Humidity of 20 - 30%

                                                         • Temperature of 16 to 27°C (60 - 80° F)

                                                         • Dust level relatively low

                                                         • Requires bench space of approximately 29 x 15.25               inches (74 x 39 cm).

Frequency                                         The nominal frequencies of the Ultra WISP are 50 or 60       Hz; the operable frequency range is the same for both of                                                          these frequencies: 47 - 63 Hz.

Voltage Requirements                      Nominal Line                  Operable Voltage

                                                         Voltage                           Range

                                                              100                                 85-130

                                                              120                                 85-130

                                                              220                                 185-265

                                                              120                                 185-265

Fuse Requirements                          Nominal Line    Fuse                 Fuse

                                                         Voltage             Required          Size

                                                         100                   one 3-amp       0.25x1.25 inches (6x32mm)

                                                         120                   one 3-amp       0.25x1.25 inches (6x32mm)

                                                         220                   two 1.5-amp     5x20mm (0.20x0.78 inches)

                                                         240                   two 1.5-amp     5x20mm (0.20x0.78 inches)

Communications Connectors          IEEE-488: For connection to compatible Waters Data and   Control Stations such as the 600E System Controller.

                                                         RS-232: For injection parameter transfer to a data    module, such as the Waters 745.

                                                         Temperature Control: For future use with temperature         control components.

Input Terminals                                 The specifications for the Hold terminals are as follows:

                                                         •     TTL or switch closure compatible

                                                         •     Low trigger of 1.8 volts

                                                         •     High trigger of 3.0 volts

                                                         •     Protected to ± 30 volts

                                                         •     Minimum of 30 millisecond pulse

                                                         •     Maximum of 5 milliamps current

Output Terminals                              The specifications for the Inject Start, Run Stop, and Out     terminals are as follows:

                                                         •     Contact closure compatible

                                                         •     Maximum 10 watts with the following restrictions:            maximum of 0.5 amps current (to 20 V), maximum 130                                                          VAC (to 0.07 amps)