Manufacturer:                   Shimadzu

Model:                               LC 10A

The LC-10AD is a dual piston pump designed to improve accuracy and sensitivity when using a high performance liquid chromatograph.

In addition to a pump, an automatic or manual injector, column, injector holder, and detector are required for a high-performance liquid chromatography system. All these devices are supplied as additional special accessories.

Please note that these operating instructions are solely limited to the operation of the LC-10AD and the important accessories. As far as the operation of the optional accessories is concerned, please refer to the operating instructions for the respective devices.

characteristic

1. Ultra-low flow pulsation solvent delivery and highly accurate gradient delivery

Compared with conventional models, the strength of the flow pulsation has been significantly reduced and the pulsation cycle is noticeably shortened. This was achieved by reducing the outflow volume to a micro volume of 10 liters per piston stroke and by using a high-speed piston. These properties allow the use of pulsation-sensitive detectors (eg differential refractometers and electrochemical detectors) in the high-sensitivity range. In addition, extremely precise gradient delivery is possible in the semi-micro volume range.

2. Variety of functions for gradient delivery

Two methods, the low and the high pressure method, are available for gradient delivery. The high-pressure gradient method is extremely accurate and has minimal delay, while the low-pressure gradient method allows solvent delivery with up to four liquids at low cost.

In addition, the control of these gradient delivery methods can be performed with two different system configurations. Either use the SCL-10A system controller or control the gradient delivery from the LC-10AD (without using a system controller).

3. Use of buffer solutions

The automatic flushing device on the back of the piston seal automatically cleans the piston. When using a high salt concentration buffer solution as the mobile phase, this device effectively prolongs the life of the piston seal.

Specifications of the LC-10AD unit

1. Pump:                                              Micro volume double piston pump (approx. 10 l/stroke)

2. Solvent Delivery Methods:               Delivery at a constant flow rate or

                                                              with constant pressure.

a) Delivery with constant flow rate:

Flow Velocity Range:

                                                              0.001 - 5 ml/min (10 - 400 bar)

                                                              5,001 - 9,999 ml/min (10 - 200 bar)

Compliance with the set flow rate:

                                                              Within 2% or 2 l/min, whichever is greater

       (0.01 - 5 ml/min, 10 - 400 bar, with water and room temperature of 20°C) except for areas of low pressure gradient delivery

Flow Velocity Accuracy:                         Within 0.3% (0.1 - 5 ml/min, 10 - 400 bar with water and room temperature of 20°C)

b) Feeding with constant pressure:

Pressure input range:                             10 - 400 bar (at 1 bar intervals)

Maintaining the set pressure:                  Less than 10% or 10 bar, whichever is greater.

3. Pressure limit:                                      high/low pressure

4. Pulsation dampener:                            Internal capacity of about 200 l.

5. Materials in contact with solvent:         SUS 316 (stainless steel), Ruby, Sapphire, Teflon 9 9 graphite annealed, Hastelloy Ce, PEEK, Kahez   KelF

6. Suction filter:                                        Sieve size 10

7. Line filter:                                             Sieve size 2, capacity approx. 200 1

Time program:                                         Flow rate, pressure, EVENT and LOOP (program repetition). A total of 10 files and 320 steps.

9. Pressure reading accuracy:                2% or 5 bar, whichever is greater.

10. Piston seal cleaning flow line:          Automatic cleaning is made possible by connecting the optional automatic rinsing device.

11. Dimensions:                                     260 x 150 x 420 (width x height x depth), but protruding components are not included.

12. Weight:                                             11kg

13. Operating temperature range:           5 - 35°C

14. Operating voltage:                            P/N 228-25000-93 : 200 - 250 VAC,

                                                               100W

High Pressure Gradient Specifications

1. SCL-10A controller

1) Number of mixed solvents:               2 or 3

2) Gradient profile:                                linear, step and exponential functions; they can consist of several steps.

3) Number of program steps:                20 files and 400 steps in total

4) Program time:                                   0.01 - 9999.9 min

5) Mixing ratio input area:                     0 - 100% (in 0.1% steps)

6) Compliance with the entered mixing ratio:  1% (0 - 100%, 0.1 - 3m1/min, 10 - 400 bar, with a water - acetone mixture)

2.LC-10AD

1) Number of mixed solvents:               2

2) Gradient profile:                                linear and stairs; they can consist of several steps.

3) Number of program steps:               10 files and 320 steps in total

4) Program time:                                   maximum 999.99 min (in 0.01 min increments)

5) Mixing ratio input area:                     0 - 100% (in 0.1% steps)

6) Compliance with the entered mixing ratio: 1% (0 - 100%, 0.1 - 3 ml/min, 10 - 400 bar, with a water - acetone mixture)

Low Pressure Gradient Specifications

1. SCL-10A controller

1) Number of mixed solvents:               up to 4

2) Gradient profile:                                linear, step and exponential functions; they can consist of several steps.

3) Number of program steps:                20 files and 400 steps in total

4) Program time:                                    0.01 - 9999.9 min

5) Mixing ratio input range:                    0 - 100% (in 0.1% increments)

6) Applicable flow rate range:                0.1 - 9.99 ml/min

2.LC-10AD

1) Number of mixed solvents:               up to 4

2) Gradient profile:                                linear and stairs; they can consist of several steps.

3) Number of program steps:               10 files and 320 steps in total

4) Program time:                                   maximum 999.99 min (in 0.01 min increments)

5) Mixing ratio input area:                      0 - 100% (in 0.1% steps)

6) Applicable flow rate range:                0.1 - 9,999 ml/min