Ciphergen PBS II
| Objektnummer | B00007309 |
|---|---|
| Seriennummer | 007309 |
| Nome oggetto | Ciphergen PBS II |
| Stato | Archived Product |
Gruppo prodotti: Life Science / Biotecnologie
Status, terms of delivery and payment
Verification of devices
The second-hand devices are verified by Labexchange Service GmbH before delivery. You are receiving only fully functional devices.
Dispatch time
The stated dispatch times are the shortest possible ones for each article. The effective dispatch times can vary. The effective dispatch times will be stated in the order confirmation.
As a matter of principle, we are offering collective deliveries. The shipping time is calculated based on the position with the longest lead time. A partial delivery is possible on explicit request.
Shipping methods
Parcel services, forwarding agencies, self-pickup, delivery by Labexchange fleet.
Delivery information
Prices exclude shipping costs. Stated shipping costs are to be expected. Deviations are possible. If transport costs are not specified, please ask separately for them.
The stated transport and packing charges apply to the most favorable route if transport and are to be understood as subject to verification due to unexpected cost increases. By reason of unpredictable events, cargo rates and delivery times can change at any time and therefore have to be adapted to the recent situation. Import formalities and possible customs charges will be borne by the purchaser. Incoterm coding according to Incoterms 2010: For persons who collect the devices themselves: EXW, for dipatch by sea: CFR, by air freight: CPT, other shipments: DAP. Note for international shipments: A proof of preference/EUR1 will not be issued by us. When self-collecting/ordering EXW from countries within or outside the European Union, 16% VAT will be retained as a deposit until we have received the corresponding confirmation of arrival/bill of delivery from the buyer.
Terms of payment
We do not accept payment by letter of credit, PayPal, etc. In each case the invoice amount is payable without deduction. Discount is not granted.
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Country |
Possible payment methods |
Comment |
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DE, AT, CH |
Payment by invoice, payment in advance, payment by credit card |
Payment by invoice is only possible for corporate clients. |
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NL, BE, LU |
Payment by invoice, payment in advance, payment by credit card |
Payment by invoice is only possible for corporate clients |
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Other countries |
Payment in advance, payment by credit card |
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Our General Terms of Sale, Delivery and Payment are valid and are available for download here.
The goods are offered subject to prior sale.
Definition of status
All articles are used articles, except an article is listed especially as a new device.
|
Status |
Condition |
Comment |
|
Immediately available |
Used | The article is fully functional and in impeccable condition. It can be shipped immediately. |
| In stock |
Used |
The article is on stock. Our service technicians will verify the article before delivery. You are receiving only a fully functional article. |
|
Published |
Used |
The article is still with the provider. After your order the article will be purchased and verified by us before being shipped to you. A certificate of operativeness as well as a service report are included in delivery. |
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New device |
new |
The article is brand new and unused. Regarding new equipment the guarantee/warranty conditions of the corresponding manufacturer apply. |
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Labprocure |
Used |
Labprocure GmbH, as the advertiser, is responsible for the content of this device offer. Labprocure assumes liability for the offers advertised here and for the photos and offer texts included. Labprocure GmbH, Bruckstraße 58, 72393 Burladingen. |
The ProteinChip System
Ciphergen's ProteinChip System is comprised of a ProteinChip Reader (series PBS II) integrated with ProteinChip Software and a personal computer to analyze proteins captured on ProteinChip Arrays. The ProteinChip System detects and accurately calculates the mass of compounds ranging from small molecules and peptides of less than 1000 Da up to proteins of 500 kDa or more based on measured time-of-flight. The System is compact enough to fit into almost any lab space, allowing researchers direct access to precision mass analysis of important peptides and proteins from complex biological samples.
ProteinChip Arrays
Ciphergen's ProteinChip Arrays distinguish this technology from other mass spectrometry-based analytical Systems. ProteinChip Arrays provide a variety of surface chemistries that allow researchers to optimize protein capture and analysis. The surface chemistries of the arrays include a series of classic chromatographic chemistries and specialized affinity capture surfaces. Classic chromatographic surfaces include normal phase for generic protein binding; hydrophobic surfaces for reversed-phase capture; cation- and anion-exchange surfaces; and immobilized metal affinity capture (IMAC) for metal-binding proteins. Specific proteins of interest can be covalently immobilized on pre-activated surface arrays, enabling customized experiments to investigate antibody-antigen, DNA-protein, receptor-ligand, and other molecular interactions.
The ProteinChip Reader (Series PBS II)
The ProteinChip Reader is a laser desorption/ionization time-of-flight mass spectrometer that uses state-of-the-art ion optic and laser optic technology. The laser optics maximize ion extraction efficiency over the greatest possible sample area, and thus increase analytical sensitivity and reproducibility. The Reader's ion optics incorporate a four-stage, time-lag-focusing ion lens assembly that provides precise, accurate molecular weight determination with excellent mass sensitivity.
The ProteinChip Software
Ciphergen's ProteinChip Software controls all aspects of the ProteinChip Reader and facilitates data collection and analysis. The Software uses a Microsoft Windows NT interface, and contains numerous features, including automatic reading of ProteinChip Arrays; multiple spectrum comparison for differential protein display and biomarker discovery; several alternative viewing formats for data; and a user-friendly interface.
Technical Specifications
The ProteinChip Reader is a linear laser desorption/ionization time of flight mass spectrometer.
Enclosure
Floor-standing unit with castors.
Dimensions: 98 cm (38.5") wide x 56 cm (22") deep x 71 cm (28") high.
Mass: 145 kg (320 pounds).
Mass Analysis Range
From less than 0.1 kDa to greater than 500 kDa.
Sensitivity
Better than 300:1 average signal to noise ratio on the analysis of 50 femtomole of bovine IgG when digitally smoothed with a (0.2 times peak width) sliding window average.
Mass Resolving Power
Automatic Mode
Better than 600 average resolution for 10 "spot protocol" automated 25-shot averages of 5 picomole of human recombinant insulin.
Manual Mode
Isotopic envelope limited resolution of better than 1000 for the analysis of 5 picomole of human recombinant insulin.
Mass Accuracy
Internat Calibration, Absolute
Better than 0.02% absolute error for eight measurements of 1 picomole of bovine insulin B chain calibrated against somatostatin and human recombinant insulin on an 8-position, "A-H," normal phase ProteinChip.
Internat Calibration, Averaged 1
Better than ± 0.5 Da 0.5 to 5 kDa
Better than ± 200 ppm (0.02%) 5 kDa to 10 kDa
Better than ± 500 ppm, (0.05%) 10 kDa to 300 kDa
External Calibration, Absolute
Better than 0.1% average absolute error for seven analyses of human angiotensin I, somatostatin, bovine insulin B chain, and recombinant human insulin (~1 picomole each) as analyzed on an 8-position, "A-H," normal phase ProteinChip in which spot D is used as a calibration run.
External Calibration, Averaged 2
Better than ± 1 Da 0.5 to 1 kDa
Better than ± 1000 ppm (0.1%) 1 kDa to 10 kDa
Better than ± 2000 ppm (0.2%) 10 kDa to 300 kDa
Ion Source
Laser desorption ionization
Radial-focusing electrostatic optics
Operating voltage: 0- ±20 kV (standard software selectable settings: +20 kV, +6 kV, -6 kV)
Factory burn-in: stable to ±30 kV at termination of conditioning procedure
Flight Tube
0.8 m
Ion Detector
Minimum peak width <8 ns
Linear dynamic range: linear output to 10 9 electrons (500 mV for a 10 ns pulse)
Large-pulse recovery <30 ns
Noise <2 mV rms 0-125 MHz
Transient Digitizer
Trigger jitter: < 2 ns
Aperture jitter: <10 ps
Sampling rate: 250 MS/s or 500 MS/s
Record length: >2 ms
Input amplifiers: multi-gain linear and logarithmic
Dynamic Range
8 bits (256 signal intensity levels per scan on board; 32 bit signal averaging on computer)
Linear dynamic range software controlled in 9 steps (3-4 dB/ step) from -0.020 V to -1 V full-scale
Logarithmic dynamic range, 0 to 1 V
High Voltage Pulser
Trigger jitter: <1 ns
Lag time: 0.17-5 seconds
Pulse height: 0-5 kV
Polarity: selectable under computer control
Risetime: 100 V/ns
Pulse width: 0.1-25_seconds
Laser
Pulsed nitrogen laser (337 nm) 4 ns pulse width
Intensity adjustment: 300 steps full-scaled 0-150 mJ logarithmic.
Spot size: 200 mm x 1000 mm
Maximum pulse rate 10 Hz
Sample Handling
Linear sample arrays on 9 mm x 78.5 mm x 2.4 mm (72 mm accessible for analysis)
Vacuum load-lock for rapid sample introduction (<3 minutes from insertion to data acquisition)
Vacuum System
Base pressure <2 x 10 -7 torr (no baking).
Pressure less than 1 x 10 -6 immediately after sample exchange.
250 L/second high compression turbomolecular pump with two-stage rotary-vane backing pump.
Gauges: active inverted magnetron (high vacuum) and active pirani (low vacuum)
Control System
Windows NT-based software for automated instrument operation and data analysis
High end personal computer and monitor
GPIB interface with >750 MB/s peak data transfer rate
Data rate: 1-4 spectra per second; Analysis of one "A-H" ProteinChip Array with eight 50-spectrum averages takes approximately 10 minutes.
Supply Voltage
110-120 VAC, 10A or 220-240 VAC, 5A 50-60 Hz; 100 VAC available as option.
