PCR

Appl.Biosystems StepOne

7.900,00 €
Excl. 19% VAT , excl. Shipping Cost
9.401,00 € incl. Vat
Seriennummer: 40733
Stato: FornitoreL'elemento è ancora con il provider. Dopo il tuo ordine, verrà acquistato, controllato e spedito a te. Un certificato di funzionamento e un rapporto di servizio sono inclusi nella consegna.?The device is still with the provider. After your order is processed the device is purchased and checked by us. You get the device completely checked and with the Labexchange® full guarantee and a warranty certificate . The delivery time for this product is usually four to six weeks.
Spedizione: 4-6 weeks
Descrizione:
Life Technologies Sistema PCR in tempo reale StepOne.
48 well.
Touch screen.
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Objektnummer B00040733
Seriennummer 40733
Nome oggetto Appl.Biosystems StepOne
Stato Fornitore L'elemento è ancora con il provider. Dopo il tuo ordine, verrà acquistato, controllato e spedito a te. Un certificato di funzionamento e un rapporto di servizio sono inclusi nella consegna.
Anno do costruzione 2021
Fabbricante Appl.Biosystems
Spedizione 4-6 weeks

Status, terms of delivery and payment

Verification of devices

The second-hand devices are verified by Labexchange Service GmbH before delivery. You are receiving only fully functional devices.

Dispatch time

The stated dispatch times are the shortest possible ones for each article. The effective dispatch times can vary. The effective dispatch times will be stated in the order confirmation.

As a matter of principle, we are offering collective deliveries. The shipping time is calculated based on the position with the longest lead time. A partial delivery is possible on explicit request.

 

Shipping methods

Parcel services, forwarding agencies, self-pickup, delivery by Labexchange fleet.

 

Delivery information

Prices exclude shipping costs. Stated shipping costs are to be expected. Deviations are possible. If transport costs are not specified, please ask separately for them.

For deliveries to countries outside the EU, an additional fee of 70,00€ for export is charged. Note for international shipments: A proof of preference/EUR1 will not be issued by us. When self-collecting/ordering EXW from countries within or outside the European Union, 16% VAT will be retained as a deposit until we have received the corresponding confirmation of arrival/bill of delivery from the buyer.

 

Terms of payment

We do not accept payment by credit card, letter of credit, PayPal, etc. In each case the invoice amount is payable without deduction. Discount is not granted.

Country

Possible payment methods

Comment

DE, AT, CH

Payment by invoice, payment in advance

Payment by invoice is only possible for corporate clients.

NL, BE, LU

Payment by invoice, payment in advance

Payment by invoice is only possible for corporate clients

Other countries

Payment in advance

 

 

Our General Terms of Sale, Delivery and Payment are valid and are available for download here. 

The goods are offered subject to prior sale.

 

Definition of status

All articles are used articles, except an article is listed especially as a new device.

Status

Condition

Comment

Immediately available

Used The article is fully functional and in impeccable condition. It can be shipped immediately.
In stock

Used

The article is on stock. Our service technicians will verify the article before delivery. You are receiving only a fully functional article.

Published

Used

The article is still with the provider. After your order the article will be purchased and verified by us before being shipped to you. A certificate of operativeness as well as a service report are included in delivery.

New device

new

The article is brand new and unused. Regarding new equipment the guarantee/warranty conditions of the corresponding manufacturer apply.

manufacturer :       Appl. Biosystems

model :                   StepOne

annotation :           Dokumente engl.

 

 

 

Applied Biosystems’ 48-well low throughput real-time PCR instrument, the StepOne™ Real-Time PCR System, makes it simple and easy to get publication ready high quality data on day one. This remarkably simple real-time PCR system is designed with a user-friendly yet powerful interface for researchers new to real-time PCR. Bring total confidence to your research with the StepOne™ System that collects and saves data from all filters in every run without depending on a computer or plate setup.

  • Intuitive, flexible software and wizards guide new users through their real-time PCR experiments in three easy-to follow steps
  • Data stored both on the instrument and computer for extra safety
  • Long-life LED-based optical system records fluorescence from FAM™/SYBR® Green, VIC®/JOE™, and ROX™ dyes for gene expression analysis, pathogen quantitation, SNP genotyping, and presence/absence assays
  • Fast system with plug and play assays and reagents for maximum time savings
  • Ultra-compact footprint fits any laboratory setting
  • LCD touchscreen and USB drive with the option of PC-free instrument operation
  • Remote monitoring and email notification with run file attached for immediate access to results--even remotely
  • Easily upgradeable to the StepOnePlus™ Real-Time PCR System

New StepOne Software v2.1
Instrument software for the Applied Biosystems StepOne Real-Time PCR System runs on the Windows XP® operating system and provides instrument control, data collection, and data analysis.


Now with the Powerful Gene Expression Study Package

  • Expand your research by analyzing an unlimited number of plates in one study
  • Take advantage of the flexibility to sort data by biological or technical replicate group
  • Achieve greater accuracy with multiple endogenous controls
  • Increase precision with assay efficiency correction in gene expression analysis

Software Highlights

  • Experimental design wizards to help you design and set up experiments
  • Advanced setup if you require more flexibility for complex applications such as multiplexing
  • QuickStart setup so that you start a run immediately and enter plate information at a later time
  • Real-time monitoring of amplification growth curves enables you to view run progress
  • Remote real-time monitoring so that you can monitor progress from a remote PC with the option to download the run file
  • Email notifications with data file attached for immediate access to your results--even remotely
  • Multiple plots view so that you can simultaneously assess data from four different perspectives
  • Export easily to PowerPoint, Excel, and graphical images

Figure 1 :
The StepOne software 2.1
includes the Gene Expression
Study Package


+ View Larger Image

Remarkably Simple System
The StepOne™ system brings advanced real-time PCR technology to a new level of accessibility—even for first-time users. The system measures amplification as it occurs, cycle by cycle, allowing for precise and quantitative measurements during the exponential phase of PCR. Beginning at the StepOne™ system homepage, you can seamlessly navigate through all aspects of the real-time PCR method including sample and reaction set-up, thermal cycling, and fluorescent detection. The software is even pre-populated with the right run protocol for each application when using reagents from Applied Biosystems. Just enter your plate set up and go. Depending on the experimental design, the system can even help you select and order real-time PCR reagents online by means of convenient links in the Design Wizard. The StepOne™ System is a long life LED based optical system that records fluorescence from FAM/SYBR Green/VIC/JOE and ROX dyes for gene expression analysis, pathogen detection, SNP genotyping and presence/absence assays. This cost-effective, 3-color, 48-well system delivers precise, quantitative real-time PCR results in which you can have total confidence.

Simply Remarkable Results
Because the StepOne™ system is factory-calibrated for optical and thermal accuracy, simply remarkable real-time PCR results are available right out of the box. It can discriminate between 2 populations of 5,000 and 10,000 template copies of a TaqMan® assay with 99.7% confidence.

Applications
The StepOne™ Real-Time PCR System supports many applications including:

  • Standard Curve (absolute quantitation)
  • Relative Standard Curve
  • Comparative Ct (DDCt) (relative quantitation)
  • Genotyping and Presence/Absence
  • Melt Curve Analysis (as a standalone application)

Upgrade your StepOne™ System to a StepOnePlus™ System
The StepOnePlus™ Real-Time PCR System Upgrade is an easy upgrade solution for your StepOne™ System. You can go from 3 colors and 48 wells to 4 colors and 96 wells in no time at all. You can even receive a StepOnePlus™ instrument on loan so you can continue your research during the upgrade process.

 

Specifications

Instrument

Thermal Cycling System

Peltier-based system

Block Format

• 48-well block

Compatible Consumables

• 48-well plates with optical flat caps
• 48-well plates with optical adhesive cover
• 8-tube (0.1 mL) strips with optical flat caps
• Individual (0.1 mL) tubes with optical flat caps

Supported Volumes

10–30 μL

Sample Ramp Rate

• Fast mode: +/- 2.2 °C/sec
• Standard mode: +/- 1.6°C/sec

Peak Block Ramp Rate

4.6 °C/sec

Temperature Range

4°C-100°C

Temperature Accuracy 

+/-0.25°C (35°C to 95°C) of setpoint/display temperature, measured at 3 minutes after clock start.

Temperature Uniformity 

+/-0.50°C, 30 seconds after clock start.

Melt Curve Resolution

As small as 0.1°C

Optical System

LED, emission filters, and photodiode

Calibrated Dyes at Installation

SYBR® Green I, FAM™, VIC®, JOE™, ROX™ dyes

Passive Reference Dyes

ROX™ or any calibrated dye. Option exists to select no passive reference.

Data Collection

Data collected in all 3 filters for all wells regardless of plate setup.
Plate setup may be altered after run completes.
Fast cycling: Less than 40 minutes

Quantitative PCR Run Time

Standard cycling: Less than 2 hrs
Fast cycling: Less than 40 minutes

Touchscreen

LCD/6.5 in. Full VGA (640x480)/32K colors

Instrument Dimensions

• Width: 24.6 cm (9.7 in.)
• Depth: 42.7 cm (16.8 in.)
• Height: 51.2 cm (20.2 in.)
• Weight: 23.6 kg (52 lbs.)

Electrical

US/Canada:
• Voltage: 120 VAC ± 10%
• Frequency: 50/60 Hz ± 1%
• Nominal Current Draw: 8A
• Power: 950 W

Software 

•  System Software included at no additional cost.
Applications include Standard Curve, Relative Standard Curve, Comparative Ct (DDCt), Allelic Discrimination (Genotyping), Melt Curve, Presence/Absence (Plus/Minus). Unlimited number of software licenses provided with each instrument system.
• Primer Express Software 3.0 (to custom design primers and probes) included at no additional cost.

 

Notebook Computer Specifications

Chassis Type  

Notebook

Display Type  

15 inch SXGA+, TFT/active matrix, LCD Display

Front Side Bus / Cache  

667MHz / 2 MB L2 Cache

Wireless LAN

Dell Wireless 1390 WLAN (802.11b/g 54 Mbps)

Memory  

1.0 G DDR2-533 MHz SDRAM on 2 DIMM

Hard drives (qty 1)  

1 x 80 GB, 9.5MM, 5400 RPM

Operating System  

Windows XP Pro SP2 operating system, license on chassis, with media

External I/O Ports  

Serial, docking connector, S-Video, IEEE-1394, 4 USB, VGA, headphone/speaker out, infrared port, RJ-11, RJ-45, AC power, integrated microphone

Primary Battery

4 cell, 32WHr Lithium Ion

Security

Cable lock provided

 

Minitower Computer Specifications

Chassis Type 

Minitower

Display Type 

Dell™ UltraSharp™ 1704FPV LCD display, 17” Flat Panel

Processor 

Celeron 346/3.06GHz

Front Side Bus / Cache 

533 MHz / 256KB

Memory 

1 GB NON ECC 533 MHz DDR2 2X512

Hard drives (qty 1) 

1 x 80 GB SATA 3.0 Gb/s

DVD+RW IDE Drive 

16XDVD+/-RW

Operating System 

Windows XP Pro SP2 operating system, license on chassis, with media

External I/O Ports 

Microphone, stereo in, stereo out (back) & stereo out (front), headphone ,RJ-45 network port, 8 USB 2.0 ports, 2 front & 6 back (USFF - 7 USB 2.0 ports, 2 front & 5 back), 1 VGA port, 1 parallel port, 1 serial port
RJ45, 1 VGA out 15-hole, 2 serial 9-pin, 2 PS/2 ports.

 

Gruppo prodotti: PCR

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