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Objektnummer	B00016448
Seriennummer	016448
Nome oggetto	Amersham MegaBace 1000
Stato	Archiv

Status, terms of delivery and payment

Verification of devices

The second-hand devices are verified by Labexchange Service GmbH before delivery. You are receiving only fully functional devices.

Dispatch time

The stated dispatch times are the shortest possible ones for each article. The effective dispatch times can vary. The effective dispatch times will be stated in the order confirmation.

As a matter of principle, we are offering collective deliveries. The shipping time is calculated based on the position with the longest lead time. A partial delivery is possible on explicit request.

Shipping methods

Parcel services, forwarding agencies, self-pickup, delivery by Labexchange fleet.

Delivery information

Prices exclude shipping costs. Stated shipping costs are to be expected. Deviations are possible. If transport costs are not specified, please ask separately for them.

For deliveries to countries outside the EU, an additional fee of 70,00€ for export is charged. Note for international shipments: A proof of preference/EUR1 will not be issued by us. When self-collecting/ordering EXW from countries within or outside the European Union, 16% VAT will be retained as a deposit until we have received the corresponding confirmation of arrival/bill of delivery from the buyer.

Terms of payment

We do not accept payment by credit card, letter of credit, PayPal, etc. In each case the invoice amount is payable without deduction. Discount is not granted.

Country	Possible payment methods	Comment
DE, AT, CH	Payment by invoice, payment in advance	Payment by invoice is only possible for corporate clients.
NL, BE, LU	Payment by invoice, payment in advance	Payment by invoice is only possible for corporate clients
Other countries	Payment in advance

Our General Terms of Sale, Delivery and Payment are valid and are available for download here.

The goods are offered subject to prior sale.

Definition of status

All articles are used articles, except an article is listed especially as a new device.

Status	Condition	Comment
Immediately available	Used	The article is fully functional and in impeccable condition. It can be shipped immediately.
In stock	Used	The article is on stock. Our service technicians will verify the article before delivery. You are receiving only a fully functional article.
Published	Used	The article is still with the provider. After your order the article will be purchased and verified by us before being shipped to you. A certificate of operativeness as well as a service report are included in delivery.
New device	new	The article is brand new and unused. Regarding new equipment the guarantee/warranty conditions of the corresponding manufacturer apply.

Firma: Amersham

Modell: MegaBace 1000

Kommentar: Dokumente engl.

The following illustrations and descriptions are referring to the instrument model and are drawn from brochures. They are not representing the delivery volume. The exact delivery content you will find only in the offering text.

MegaBACE 1000 available from AME Bioscience

Description
MegaBACE 1000 DNA Analysis System is a high-throughput, fluorescence-based DNA system utilizing capillary electrophoresis with up to 96 capillaries operating in parallel. The system performs sample injection, gel matrix replacement, DNA separation, detection, and data analysis.

Components

Capillary electrophoresis instrument
96 Capillary arrays
Separation matrix for DNA analysis
Reagents for DNA sequencing and genotyping
Instrument control and data analysis software
Computer workstation and monitor for instrument operation and data analysis

Capillary arrays
The capillaries are supplied in pre-assembled arrays of 96 capillaries. 16, 32, 48, 64, 80 or 96 capillaries can be used during a run.

200 µm outer diameter
75 µm internal diameter
40 cm detection length

Separation matrix
MegaBACE Long Read Matrix is optimized for both sequencing and genotyping applications. This saves time when switching between applications.
MegaBACE Long Read Matrix can produce DNA sequences of 700 bases or resolve multiplexed fragments in excess of 900 bases. The gel matrix is replaceable, and each pack includes 96 pre-dispensed tubes plus running buffer.

Sequencing reagents
All MegaBACE DNA sequencing reagent kits offer unparalleled performance on a broad range of templates:

DYEnamic ET Terminator Kits with Thermo Sequenaseú II DNA Polymerase
DYEnamic ET Primer reagents

Genotyping reagents
To complement instrument functionality, we offer a range of MegaBACE ET Size Standards for a variety of applications. These include MegaBACE ET400-R, ET550-R, and ET900-R.

For SNP validation, MegaBACE SNuPe Genotyping Kit contains everything needed to perform a robust single-base extension reaction of PCR products in a single-tube format.

Software
The following software tools are available:

Instrument Control Manager is used for instrument set-up and sample entry.
Sequence Analyzer processes large amounts of data without manual intervention.
MegaBACE Sequencing ScoreCardú offers a time-saving overview of all samples.
MegaBACE Genetic Profiler reduces manual data review.
MegaBACE Genetic Profiler ScoreCard allows quality comparisons between capillaries and runs.
MegaBACE SNP Profiler saves editing time by assigning quality scores to each SNP call.

Computer requirements
Hardware: Pentium Pro 220 MHz or higher
Operating system: Windows NT 4.0 or Windows 2000
Installed RAM: 64 MB or more
Software: Microsoft Office Pro

Specifications
Sequencing read length and throughput
With the ability to sequence 96 samples in just 2 hours and perform up to 9 runs per day, MegaBACE 1000 is the ideal choice for production-scale sequencing projects. Average read length with dye primer chemistry is 550 bp at 98.5% accuracy with M 13 standard template. With a slower, 3-hour run time, sequences of up to 1000 bases is possible. MegaBACE 1000 can produce more than 500 samples per 9-hour day.

Genotyping read length and throughput
The MegaBACE DNA Genotyping System automatically processes 2000 microsatellite genotypes per 96-well run. The total turnaround time per run is less than 2 hours from injection to injection. This corresponds to more than 14 000 genotypes per system per day. For SNP analysis, MegaBACE 1000 can deliver
>5 760 SNPs per day.

Sample requirement
Minimal sample loading volume of 5 µl. The samples are introduced to the system in a 96-well format. Samples can be reinjected up to 10 times.

Excitation

Single mode (488 nm) for sequencing
Dual mode (488 and 532 nm) for genotyping

Detection

4 channels with 2 PMTs
Confocal optics with multiple filter sets
Digital resolution is 16 bits linear to 5%

Electrophoresis Voltage: Up to 320 V/cm
Chamber Operating Temperature: 27 ¡C to 44 ¡C

Environmental Requirements
Temperature: 20 ¡C to 25 ¡C
Humidity: 80% noncondensing
Weight: 272 kg (approximate)
Dimensions: 81.2 cm (height) x 103.3 cm (width) x 87.4 cm (depth)

Gruppo prodotti: Sequenziatore DNA

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