manufacturer:       Messer Griesheim/Planer

model :                   Kryo 360-3.3

annotation:           Dokumente engl.

The Kryo 360 incorporates all of the critical features expected from a high class biological freezer. The -180 ºC end temperature ensures sample integrity during transfer to storage whilst the flexibility of the system is ideal for the more complex and demanding protocols associated with the most advanced cryopreservation techniques.

The high capacity LNP4 active nitrogen pump offers both faster cooling rates and a large reservoir, offering the reassurance of an extended hold time at the protocol end temperature. The system sample capacity is sufficient for the busiest laboratory and the state of the art compact design will enhance the most modern facility.

The MRV controller system has been created to offer multiple protocols whilst remaining simple to programme and operate, and both during and after a run offers the widest range of displayed information, alphanumerically and graphically via the easy view display and as a print out on the integral full view printer.

Validation is a high priority and the MRV offers password controlled access on multiple user levels, time and date stamping, programme preview and verification before running and data storage for the last 10 runs for subsequent printing. User calibration with associated hard copy is featured and PC connection compatible with the comprehensive Delta T™ software application is standard.

The system has been fitted with numerous safety features. These help protect against power failure and PC failure when running with software. Processor or system problems are controlled and the system restarts to protect samples - for example all control and data systems are separated, the controller can be removed from the operating freezer with no loss of programme integrity; data storage and run processing are completed on isolated electronic systems.

Overview

●  Chamber volume: 1.7 or 3.3 litres
●  Straw capacity: 60 or 120 x 0.25ml straws / 45 or 90 x 0.5ml straws
●  Ampoule capacity: 30 or 60 x 2ml ampoule
●  Lower temperature limit: -180ºC
●  Cooling rates: -0.01 to -50ºC/Min
●  System Pump: LNP4-P
●  System Dewar: LAB20
●  PC Software: Delta T™

■  Designed for freezing of embryos and sperm in straws and ampoules
■  Controller displays demand, sample and chamber temperatures, programme
       stage and current temperature graphic
■  Menu driven controller, simple to programme and operate
■  Horizontal or vertical operation
■  Compact design
■  Standard operating features:-

   1. Start above ambient
   2. Controlled heating
   3. Data Printing (integral printer)
   4. Comms port for PC connection
   5. Fast cooling rates
   6. Multiple safety features

System Specification

Range                                  +40 °C to -180 °C

Heating rate                          0.01 °C/min to 10 °C/min

Cooling rate                          -0.01 °C/min to -50 °C/min

Controller accuracy             ±0.3 °C measured on a hold at 0 °C

Storage temperature            -10 °C to +50 °C

Storage humidity                  5 % to 95 % relative humidity non-condensing

Operating temperature        5 °C to 40 °C

Operating humidity               5 % to 90 % relative humidity non-condensing

Controller Specification

Dimensions                          80 mm high x 220 mm wide x 350 mm deep

Weight (approx.)                  2.6 kg

Display                                 240 x 64 LCD with CCFL backlight

Printer                                  320/640 dot thermal printer

Keypad                                 20 key membrane keypad

Programmable Cooling Rate Range -0.01 °C/min to -99.9 °C/min

Number of profiles               10

Steps per profile                   32

Number of stored runs         10

Chamber Specification

Weight (kg)                          14.4 or 14.7

Capacity (litres)                    1.7 or 3.3

Chamber dimensions          1.7L Chamber: Internal 200 mm x 150 mm diameter

External 450 mm high x 300 mm wide x 420 mm deep

3.3L Chamber: Internal 400 mm x 150 mm diameter

                                            External 450 mm high x 300 mm wide x 420 mm deep

0.25 ml straws                     60 or 120

0.5 ml straws                       45 or 90

2 ml ampoules                     30 or 60

50 cc blood bags                 -

250 cc blood bags               -

500 cc blood bags               -

Power requirements (inc. MRV Controller)    115V~ 50/60Hz 600VA / 230V~ 50/60Hz 600VA

Recommendation of additional equipment 5 psi System         System Pump - LNP4-C

                                                                                                              System Dewar - MVLAB 30

                                                                               22 psi System      System Cylinder-MVEUROCYL230SB

                                                                                                              Phase Separator - MVPHASE

(Alternative)                                                          22 psi System      Vacuum Jacketed Pipe Work System

                                                                                                              Phase Separator - MVPHASE