Firma:              Chrompack

Modell:            Grass

Kommentar:    Dokumente engl.

The device is modular designed. Please, find below the modules you are looking for.

UV VAR Detector

CAPABILITIES

The unit provides a unique combination of ease of use with power and flexibility in a single, microprocessor controlled package:

operation as a simple, variable wavelength UV-visible absorbance detector. The detector may be set to any wavelength in the 190 - 800 nm range. Chromatographic data may simultaneously be output on two recorder channels at different sensitivities and on a fixed-sensitivity analog channel for use with oomputing integrators.

operation as a scanning UV-visible detector. At any time, a full spectrum of the cell contents can be scanned and output to a recorder channel

operation as a recording detector. Chromatographic data can be stored in memory for later replay at any sensitivity, speed, or rise time.

operation as a recording spectrophotometer, in which spectral data may be acquired and stored in memory for later replay.

operation as a time-programmed detector /spectrophotometer in which wavelength, sensitivity, and response time may be changed or spectra scanned at predetermined times during a chromatographic run.

dual-wavelength operation, in which chomatographic data may be acquired at two different wavelengths. The absorbances at the two wavelengths are simultaneously plotted along with an absorbance ratio upon a 3-pen recorder.

automatic peak detection for sample collection.

baseline correction for drifting baselines.

All of the instrument operations can be controlled by the user from the front panel keypad. The large display screen and menu-driven operation combine to make it easy to use the power bunt into the system.

Finally, the convenient location of the lamps, detector, and flow cell housing make changing any of these components easy.

SPECIFICATIONS

Wavelength:                            190-800 nm

Bandwidth:                              6 nm

Wavelength Accuracy:            + or -1 nm

Wavelength Precision:            + or - 0.1 nm

Optical Methodology:               Deuterium (190-365 nm) and tungsten (366‑800) lamp light sources with concave holographic grating monochromator and double beam optics, provision for purging the monochromator

Range Selections:                   0.0005 to 7.9995 AUFS, in 0.0005 AU increments on any of the chart recorder outputs

Absorbance Ratio:                  Displayed as ratio of absorbances (at the two selected wavelengths) per full scale range; pbtted as log of ratio of absorbances per full scale range

Recorder Outputs:                  10mV, 100mV, or 1V output in any combination on the four recorder outputs

Integrator Output:                    1V per AU, Selectable for bare or delta wavelength, auto zero capability

Remote Controls:                    Rear panel input for auto zero of the chart recorder outputs. Rear panel input for event mark. RS-232C serial interface for computer control and/or data acquisition. DPDT relay closure for optional stop tlow valve control and/or peak detection driven fraction collector. Chart recorder control.

Noise:                                                  <2 x10 ^-5 AU from 220-280 nm with 1.0 sec rise time. Single wavelength Operation.

            <5 X10 ^-5 AU from 220-280 nm with 1.0 sec rise time. Dual wavelength operation.

Drift:                                         <2 x 10 ^-4 AU/hr after warm-up*

Zero AdJust:                            Plus or minus any amount by autozero or by manual entry

Chart Recorder Filter:             Digital active filier with rise times selectable from 0.0 to 9.9 sec (divide by 2 to convert to an equivalent time constant)

Flow Cells:                              Pathlengths from 10 mm to 0.0 mm, cell volumes from 0 to 15 microliters, stainless steel or Kel-F contact materials, sapphire windows; 1000 psi pressure rating for stainless steel cells, 500 psi pressure rating for Kel-Fim celts, 2000 psi pressure rating for variable pathlength preparative cell; microbore cells available.

Programmability:

• 256K of chromatogram and spectra storage
• take spectra at any scheduled time
• change wavelength at any scheduled time
• store spectra and chromatograms
• "playback" stored data at any range, rise time, or rate on any recorder channel
• store four complete programs in nonvolatile memories

Fluorescence Detector

GENERAL DESCRIPTION AND PRINCIPLE OF OPERATION

General Description

The Chrompack fluorescence detector is a high performance fluorescence detector designed to provide the high sensitivity reguired for HPLC fluorescence analysis, and as such it incorporates the following features:

Optical design for greater sensitivity

1. The highly energy-efficient optics uses no lenses and thus avoids the problem of reflection loss and chromatic aberration.
2. Simple optics using holographic, concave diffraction gratings and aspherical mirrors provide highly efficient monochromators in a compact package.
3. Use of a 150 W xenon lamp provides greater light energy for excitation.
4. Slit width of the Em monochromator may be changed in two steps (normal, 18 nm, or wide, 30 nm, spectral band width) via front panel key pad, allowing to select the most suitable band width for the analysis.

Microprocessor control for greater versatility and reliability

1. The wavelength is set using increment/decrement keys and an LCD multi-display; this facilitates setting the correct parameters thus increasing the reliability of measurement.
2. Operating status and the results of self-diagnostics are displayed as messages on the LCD multi-display.
3. Digital processing is used, not merely for control, but throughout the entire signal processing system, assuring higher reliability and operational stability.
4. Equipped with a microprocessor-controlled auto-zero system which provides a wide range of base settings.
5. Excitation and emission wavelengths, Gain, Attenuation may be changed in five steps according to a time program, allowing optimum conditions to be set automatically for each peak component.

Special functions not provided on previous HPLC detectors

1. 10. Emission and excitation wavelength sweep function makes it possible to obtain a broad emission spectrum and excitation spectrum.
2. 11. When equipped with the optional rectangular cell holder, the emission signal values displayed on the LCD multi-display allow the unit to be used as a simple fluorescence detector.
3. 12. The integrated lamp operation time is displayed on the LCD, assuring higher reliability and safety of the light source.

Principle of operation

The detector consists of two basic sections, an opticaI section and a control section.

The optical section consists of a light source, a beam condenser, excitation monochromator's illumination optics, a flow cell, emission collector optics, an emission monochromator, and a detector.

UV light from the light source (LS) is concentrated and directed towards the excitation monochromator excitation beam entrance slit (SI) by an elliptical mirror (MI). The excitation monochromator disperses the light to obtain a dispersed emission spectrum, which is directed towards the flow cell by the illumination optics. A part of the energy of the excitation beam is split by a beam splitter (e) and directed towards a photo-diode (PD), which converts the light to an electrical signal which is then used for feedback to the light source. Light emitted from fluorescent substances in the flow cell is collected and focussed on the emission monochrometer slit (S5) by the emission collector optics, where the dispersed emission spectrum is directed to the detector (PM) for conversion into an electrical signal.

The control system consists of a microprocessor and interfaces for connecting to other components.

Optical signals from the excitation and emission monochromators are converted to electrical signals by a photo-detector and photo-multiplier, amplified by a preamplifier, and then converted to digital signals by an A/D converter. The microprocessor calculates the ratio of the two signals to compensate for the fluctuations in the light source intensity, then sends resultant values to a D/A converter for output to the integrator and recorder. Commands from either the keypad or time program are processed and executed by the microprocessor. A block diagram of the control system is shown in figure 2-2.

SPECIFICATIONS

Chrompack Fluorescence Detector

Optical system

Monochrometer                       Holographie concave diffraction

                                               grating (for both excitation and emission)

Light source                            150 W xenon lamp

Wavelength range                   220 nm - 700 nm (excitation and emission)

Spectral band width                Excitation: 18 nm (fixed);

                                               emission : 18 nm, 30 nm (selectable)

Wavelength accuracy             + 5 nm

Wavelength

reproducibility                          ± 0.3 nm

Detectors                                Photo-multiplier, reference: photodiode

Control system

Detector sensitivity                  Raman peak S/N 120 for water

            ( with 350 nm excitation wavelength and time eonstant of 1.5 sec)

Sensitivity

attenuation range                    1, 2, 4, 8, 16, 32, 64, 128 and Short

Amplifier range                        x 1000, x 100, x 10, x 1

Response                                Fast (0.1 sec), standard (1.5 sec) and slow (3.5 sec)

Signal processing                   Digital processing using A/D and D/A convertors

Outputs                                   Recorder output - 10 mV/FS;

            integrator output - 1 V/FS or 0.5 V/FS; marker output and leak output

Inputs                                       Marker input and auto-zero input; program reset/run input

Program functions                  Ex and Em wavelengths, gain, attenuation is controlled by the time program in five steps (including the initial conditions); program time is up to 999.9 minutes (in 0.1 min increments).

Ex sweep function                   Allows automatic scanning of the Ex wavelength for Ex spectra output.

Em sweep function                 Allows automatic scanning of the Em wavelength for Em spectra output.

Self-diagnostic

functions                                  Memory (ROM, RAM), DC power supply,

                                               Ex energy reduction, cell leakage

Integration lamp

operation time                         Integrated Xe lamp operation time is displayed on the LCD.

Polarity change function:        Recorder output polarity may be changed alternately via front keyboard.

Other

Liquid wetted materials          Quartz, fluoroearbon resin or stain1ess steel

Dimensions and weight          210W x 225H x 470D; approx. 12 kg

Power requirement     110, 220 or 240 V AC, 50/60 Hz 350 W maximum

CP ISOS/GRAS LC Pump Operator Manual

INTRODUCTION

CHROMPACK ISOS and GRAS Pumps have been designed for ease of use and unsurpassed performance. They can be used as stand-alone pumps or as modules in a totally automated LC System. The GRAS offers low pressure ternary mixing that provides accurate proportioning of binary or ternary mobile phase compositions in either the gradient or isocratic mode. The isocratic ISOS pump offers all of the features and performance of the GRAS pump except that the ternary proportioning valve, along with its associated electronic circuitry and Software, is not included in the ISOS.

The GRAS and ISOS pumps permit as many as 40 lines of programming in each of 10 method files, which are sufficient for even the most complex of gradient profiles.

The built-in Startup File can be used to transform the pumps into true turn-key systems. The Startup File remains resident in the unit even when the power is switched off, and it is automatically reloaded whenever the power is switched back on. Simply turn the pump power switch on and press “INITIALIZE” and “ENTER” for chromatography to begin.

Both pumps are engineered for reliability and ease of maintenance. The pumps are designed to make proper maintenance easier than ever, thus insuring that your chromatography results not only are accurate but also remain accurate. A built-in Maintenance Log allows you to follow the lifespan and wear of specific parts (seals, pistons, and check valves) and alerts you whenever routine maintenance is scheduled.

Another Standard feature is the ability to automatically initiate a Cleanup File at the end of the run to flush out the System and clean the column. Should service ever be required, the resident diagnostics and modular design of these pumps will reduce downtime to the absolute minimum. The simplicity and durability of the Pumps translate into fewer service calls and a minimum of spare parts that need to be kept on hand.

The Flow Stability Test, described under "MONITORING THE PUMP PERFORMANCE" in Section 3, greatly enhances your ability to track the pump performance. The test can be run either continuously or automatically prior to each injection with the results documented on every integrator report if desired.

Between the proportioning valve and the firnt liquid end on the GRAS pump, there is an inlet tube assembly that provides mixing volume for the gradient solvents. To provide additional mixing capability, a molded mixing strip is packed in the front end of the tube. The mixing strip is made of Tefzel ^® plastic and is about four inches Jong. Its square-tooth saw design provides enough agitation to the solvent flow to improve the solvent rnixing and reduce the pump dead volume by about 120 µL.

For applications where larger mixing volumes are needed, a static mixer (Filter/Mixer) or a dynamic mixer can be readily installed on the GRAS pump, as described later in this section.

Specification

Pump Design                                      Dual piston, pulseless design with 12,800 control steps per cam revolution.

Floating Piston Design            Long seal life.

Bright Fluorescent Display      2 lines, 48 characters.

"Bayonet-Mounted"                  Easy maintenance and parts

Liquid Ends                             replacement.

Flow Range                             0.01 to 10 mL/min standard. Up to 30 mL/min using prep option. Set-table in 0.01 mL/min increments.

Flow Precision                        0.2% RSD ^a

Maximum Pressure                 6000 psi for standard head;

                                               2500 psi for prep option.

Compressibility Compensation Automatic

Method Files                            Ten method files of 40 steps each.

                                               Maximum of 370 steps for one file.

Startup File                              User defined file that is stored in non-volatile memory.

Cleanup File                            User prograrnmed file initiated automatically at end of sample run to clean out pump and column.

Flow Stability Test                   Pump flow diagnostic to verify proper pump performance.

Diagnostics                                         Diagnostic tests to verify proper electrical and mechanical operation.

Help Screens                          Over 430 lines of Help text provides specific information for all pump operations.

Communications                     Contact closures standard.

                                               RS-232—C optional.

Battery Backup                        100—hr backup of RAM memory.

Battery Backup                        Height— 7 1/4 inches

                                               Width— 15 3/4 inches

                                               Depth— 20 inches

Weight                                     33 pounds

Power Consumption               200VA

GRAS Only:

Pump Design                          Micro-stepped pump with low pressure ternary mbdng capability. A helium degas manifold is standard.

Internal Volume                       800 microliters.

Composition Range                0 to 100% in 0.1% increments.

Composition Repeatability      0.2% RSD ^b

Composition Repeatability      0.2% RSD

Gradient Linearity                    0.5% maximum deviation from linearity. ^c

Gradient Delay                        Dead volume compensation settable in 0.01 mL increments.

Minimum Flow for Gradient    0.05 mL/min.

High Internal Mixing                 No mixer required.

a   Typical RSD obtained on chromatographic retention times.

b   Typical RSD obtained on chromatographic retention times using solvent proportioning.

c   Typical deviation from the best fit linear line from 15% to 85% gradient composition without a mixer.