Firma:              BioRad

Modell:            Gene Pulser II

Kommentar:   Dokumente engl.

Gene Pulser II System Description

The new Gene Pulser II system is a completely redesigned electroporation system, which offers improved performance, flexibility, sample protection, and safety. The Gene Pulser II system uses the Pulse Trac waveform delivery system to generate the most accurate exponential decay pulses possible for optimal cell transformation within an electroporation cuvette. The Pulse Trac delivery system accurately calculates the time constant of each pulse based on what is actually delivered to the sample.

The revolutionary Pulse Trac waveform delivery system provides:

• Sample conductivity measurement integrated wich pulse output for true waveform delivery regardless of sample

• Internal calibration and circuit monitoring program for accurate pulse delivery throughout the lifetime of the unit

• Improved time constant accuracy

Other features of the new Gene Pulser II system include:

• Error free assembly, since each module uses unique cable connectors, keyed to fit only in the proper orientation and location

• Automatic display of time constant after every pulse, to make it easier to record this essen­tial pulse parameter

• Easier optimization of novel prokaryotic applications wich a wider range of high voltage capacitors (1.0, 3.0, 10, 25, and 50 µF) in the Gene Pulser II unit

• Pinpointing of optimal field strengths, with voltage selection in 2 V increments for mam­malian cell electroporation (low voltage/high capacitance) and operation at a higher maximum voltage (up to 500 V). In addition, voltage selection in 10 V increments for bacterial (high volt­age/low capacitance) electroporation

• Increased control of high voltage pulses used for bacteria and yeast electro-transformation wich the five new resistors in the Pulse Controller PLUS module

• Expanded range of precision capacitors for reproducible mammalian cell electroporation; 25 µF-3,275 µF with the Capacitance Extender PLUS module or 25 µF-1,075 µF with the Capacitance Extender II module. This permits greater time constant reproducibility and flexibility with electronically calibrated capacitors in 25 µF increments. At any time, the Pulse Trac circuitry can verify and calibrate these capacitors within ±10% variation for the ulti­mate in pulse reproducibility and precision.

• Designed and certified to meet IEC 1010 safety standards

• Entire system is completely protected from sample arcs, regardless of module or sample used

• Expanded safety, with charges automatically diverted safely to ground whenever the circuit is interrupted during pulse delivery.

The Gene Pulser II unit may be used with either of two new types of accessory modules. For electroporation of bacteria and yeast, use the Pulse Controller II or Pulse Controller PLUS mod­ule. For electroporation of mammalian cells and embryonic tissue, use the Capacitance Extender II or Capacitance Extender PLUS module.

Specifications