manufacturer:      Becton Dickinson

model:                  FACSJazz

The following illustrations and descriptions refer to the instrument model and are drawn from brochures. They do not represent the scope of delivery. Please refer to the text of the offer for the exact scope of delivery.

Technical Specifications

Installation Requirements

Dimensions (H x W x D)

Cytometer: 53.3 x 50.8 x 50.8 cm (21 x 20 x 20 in.)

Table (optional): 94.0 x 121.9 x 81.3 cm. (37 x 48 x 32 in.)

Computer cart (optional): 94.0 x 71.1 x 66.0 cm (37 x 28 x 26 in.)

Biological safety cabinet (optional): 233.4 to 249.9 x 136.5 x 87.6 cm (91.9 to 98.4 x 53.8 x 34.5 in.)

Weight

Sort head does not exceed 56.6 kg (125 lb)

Cytometer system, excluding options, does not exceed 151.5 kg (334 lb)

Temperature Operating Range

15ºC–25ºC (59ºF–77ºF)

Temperature changes within this range can affect the surface tension of fluids, and may require the operator to make minor adjustments to the system.

Power

One dedicated power line:

100/120/220/240 VAC, 50–60 Hz

Optional biological safety cabinet, aerosol management option, sample temperature control, air compressor, and vacuum supply may require an additional line. Power consumption: <600 VA

Humidity

55% ±10% relative humidity (non-condensing)

Heat Dissipation

The BTU ratings at 120 VAC for the components are as follows:

Cytometer: 1,638 BTU or 480 Whr

Air compressor (optional): 3,275 BTU or 960 Whr

Vacuum pump (optional): 1,720 BTU or 504 Whr

Sample temperature control (optional): 4,709 BTU or 1,380 Whr

Air Supply

A laboratory air source of compressed air must deliver clean (less than 5 PPM), dryfiltered (oil-free) air with stable pressure of 40–90 psi (2.5–6.2 bar) regulated. An air source option is available.

Vacuum Supply

A laboratory vacuum source must supply between 5–15 in. Hg at 1 CFM.

A vacuum source option is available.

OPTICS

Excitation Optics

Optical Platform

Each laser beam is aligned independently and has its own final focus lens foroptimal tuning.

The 488-nm laser comes standard. The 405-nm laser, 561-nm laser, and 640-nm laser are optional.

Power Out of the Laser Head

405 nm: ≥50 mW. 488 nm: ≥80 mW. 561 nm: ≥75 mW. 640 nm: ≥50 mW

Emission Optics

Steering Optics

Emission light is collected through a 20X, 0.6 NA microscope objective. Light is focused on spatially separated mirror pinholes. Simultaneous video observation of the stream, pinholes, and laser intercepts allows for fast and intuitivealignment.

Forward Scatter Detector and Filters

Collected using 75- and 50-mm lenses and a photomultiplier tube (PMT) with a 488/10 bandpass filter and an OD3 neutral density filter.

Side Scatter Detector and Filter

Collected through the 90° collection lens and measured using a PMT with a 488/10 bandpass filter.

Fluorescence Detectors and Filters

Collected through the 90° collection lens and measured using PMTs.

Fluidics

General Operation

• The fluidics system uses external air pressure and vacuum.
• An optional air pressure supply and/or vacuum pump is available.
• Sheath pressure is fixed at 27 psi (1.8 bar). Consumption of sheath fluid is approximately 0.5 L per  hour.

Fluidics Reservoirs

Autoclavable 7-L sheath and waste containers, equipped with pressure and vacuum readout, are provided.

Fluidics Control

• A sample flow fine adjustment is provided for precise regulation of the sample flow.
• Purge, pulse, rinse, and run functions are provided for quick stream startup and bubble removal.

Nozzles

100 μm (2 included with the instrument)

Replaceable Fluidics Path

• The fluidics path, including the nozzle assembly and sample line, can be exchanged. There are no inline valves. Only pinch valves are used.

Bubble Detector

A bubble detector in the sample line detects air bubbles from the sample tube and stops sample flow when the sample tube is empty, preventing air bubbles from reaching the nozzle assembly.

Sample Input

• Recommended minimum sample volume is 0.2 mL. The dead volume is approximately 25 μL.
• 12 x 75-mm polypropylene tubes

Performance

Fluorescence Sensitivity

Fluorescence sensitivity was measured using SPHERO™ Rainbow Calibration

Particles according to the manufacturer’s specifications:

FITC: 125 molecules of equivalent soluble fluorochrome (MESF-FITC)*

PE: 125 molecules of equivalent soluble fluorochrome (MESF-PE)*

Fluorescence Resolution

Measured with propidium iodide (PI)

-stained chicken erythrocyte nuclei (CEN) and Pulse Processing/Integrator option. Coefficient of variation (CV) of PI fluorescence area: <3.0% full G0/ G1 peak. Use of the integrator option enables differentiation of G2/M singlets from G0/G1 doublets for PI stained calf thymus nuclei (CTN).

Fluorescence Linearity

Doublet/singlet ratio: 1.95–2.05 for CEN stained with PI

Forward and Side Scatter Sensitivity

Sensitivity enables separation of fixed platelets from noise, and detection of 1.0-μm beads.

Forward and Side Scatter Resolution

Scatter resolution enables differentiation of lymphocytes, monocytes, and granulocytes.

Sort Performance

Drop Drive Frequency

37–41 kHz

Purity and Yield

At 27 psi and 39 kHz with an average threshold of 10,000 events per second, a two-way sort achieved a purity of 98% and a yield of >80% of Poisson’s expected yield for both populations. Higher thresholds up to 20,000 events per second can be achieved without affecting purity; however, yield will decrease based on Poisson’s statistics.

Viability

Sorts of Jurkat cells resulted in >90% viability and similar proliferation rates * MESF

Data Management

Software

BD FACS Sortware sorter software

Workstation

PC workstation with at minimum: Intel® 3.3GHz Quad Core CPU, Microsoft®Windows® 7 Professional 64-bit operating system

Memory

8 GB of RAM

Data Storage

2 x 300 GB hard drives, RAID 1 (mirrored) configuration 8X DVD+/-RW 7 USB 2.0

Networking

(10/100/1000) GB Ethernet

Monitor

27-inch LCD, 2560x1440 resolution

Groupe de produits: Cytomètres en flux