manufacturer:       Xiril

model:                   X100-1-4

annotation:           Dokumente engl.

MRSA extraction protocol on the Xiril robotic workstation

MRSA (methicillin resistant Staphylococcus aureus) is a resistant variation of the common bacterium Staphylococcus aureus. It has evolved an ability to survive treatment with beta-lactam antibiotics and is especially troublesome in hospital-associated infections. Consequently most hospitals screen samples from both patients and other possible contamination sources. These samples are first cultivated, then boiled and finally analysed by qPCR (quantitative polymerase chain reaction).

Xiril offers an optimal implementation of the DNA extraction on its highly accurate robotic workstations and allows a high throughput of samples with small input of time and manpower. This fully automated method allows the extraction of 96 samples within 50 minutes and enables a straightforward and cost-effective solution. Furthermore it offers the possibility to implement other actions such as the PCR setup without major investments.

Key Features:

• High capacity, 96 samples within 50 min (eight pipettes)
• Ready to use system
• Reliable and reproducible operation
• Low cost: end user price for one extraction is approx. 1 € (including tips) Optional sample identification

Capacity and Throughput

The Xiril robotic workstations are easily configured to meet different demands for capacity and throughput. Two deck sizes (100-series and 150-series) and the option of four or eight pipettes provide the flexibility to readily meet your requirements. Refer to table 1 below for approximate throughputs for 96 samples for two different instrument configurations.

Table 1: Throughputs for 96 samples for the 100-series instruments with 4 or 8 pipettes.

Instrument                                                                  Run duration [minutes]

---------------------------------------------------------------------------------------------------------------------------

100-series, 1 arm, 4 pipettes                                    75

100-series, 1 arm, 8 pipettes                                    50

Automated Processing Requirements for the 100-series with One Robotic Arm and Four Pipettes

XIRIL ROBOTIC WORKSTATION DECK SETUP

This is an example of the MRSA extraction deck layout on a 100-series-1-4 robotic workstation for use with one plate (96 samples). The layout can be easily configured to user’s needs with the flexibility to position hardware and labware freely on the instrument deck. If needed the sample identification and tracking can be enabled by different methods: Import of a sample file (e.g. LIMS), which is implemented in the Lirix software, handheld barcode reading, plate scanning (100- and 150-series) and finally tube scanning (only available for 150-series). Please refer to the figure below for the recommended deck layout.

Figure 1: Deck layout for MRSA extraction kit on the Xiril 100-1-4 robotic workstation.

1: Module Decktray,

2: Thermo-ShakeX with PCR 96-well plate,

3: Millipore manifold and filter plate,

4: High Precision Decktray,

5: 250 µl Rainin filter tips,

6: Plate-GripX and liquid waste,

7: High Precision Decktray,

8: Reagent rack incl. 6 troughs,

9: Tip waste station,

10: Standard Decktray,

11: 24-well microplate,

12: SPE Workstation,

13: 96-well microplate,

14: 96-well Millipore filter plate

Sequencing reaction clean-up CleanSEQ® system on the Xiril robotic workstation

CleanSEQ® is the Agencourt dye-terminator removal kit for rapid, high performance sequencing clean-up. It is based on a magnetic bead format technology where the nucleic acids are immobilized onto paramagnetic micro particles using specific buffer conditions. Thanks to this solid phase reversible immobilization (SPRI), sample contaminants are easily removed without the need for labor-intensive centrifugation or filtration, consequently creating an automation-friendly format. The Xiril robotic workstation offers an optimal implementation of the Agencourt purification method on its highly accurate robotic workstation. In order to meet your requirements, Xiril proposes a high sample throughput with a reliable and cost-effective solution and the possibility to implement other actions such as the PCR setup without major investment.

Key Features:

• High quality sequencing throughput
• Reliable and reproducible operation
• High capacity, 96 samples within 50 min. (eight pipettes)
• Ready to use system
• Low tip throughput
• Sample identification possible
• Low cost solution
• Option to implement the PCR setup on the same workstation

Automated Processing Requirements for the 100-series with One Robotic Arm and Four Pipettes

XIRIL ROBOTIC WORKSTATION DECK SETUP

The figure 1 is an example of the deck layout on a 100-series-1-4 robotic workstation for the clean-up of 96 samples. The layout can be easily configured to user’s needs with the flexibility to position hardware and labware freely on the instrument deck. For example it is possible to process 192 samples in one run just by upgrading the following workstation with two additional decktrays and an additional SPRI plate as well as more tip boxes. A further possiblity to increase the run speed can be to use a workstation with two arms, one handling eight pipettes and the other a 96 tip head. Finally the sample identification and tracking can be enabled by different methods: The import of a sample file (e.g. LIMS), which is implemented in the Lirix software, handheld barcode reading and plate scanning (only available for 100- and 150-series).

Figure 1: Deck layout for sequencing reaction clean-up on the Xiril 100-1-4 robotic workstation.

1: Module Decktray,

2: Tip waste station and liquid waste,

3: Re-Use rack for tips,

4: Plate-GripX,

5: High Precision Decktray,

6: 250 µl Rainin filter tips,

7: Standard Decktray,

8: 96-well thermal cycling plate,

9: Magnetic plate 96 (SPRI),

10: 96-well thermal cycling plate,

11: Reagent rack incl. 6 troughs.

DNA IQTM System on the Xiril robotic workstation

Introduction

DNA analysis has widespread applications in the clinical, research and forensic field. Manual methods for the purification of a big number of nucleic acids can be laborious and time consuming. Xiril offers an optimal combination of the reliable Promega DNA extraction method with its highly accurate robotic workstation. This fully automated method based on a paramagnetic resin for DNA isolation allows a high sample throughput with a reliable and cost-effective solution. The Promega system is designed to rapidly purify small quantities of DNA and give consistent yields for a specific sample type. Together with the Xiril robotic workstation it allows the walk away extraction of up to 96 samples DNA within 70 minutes.

Key Features:

• Isolation of a consistent DNA amount from a broad variety of samples
• Reliable and reproducible operation
• High capacity, 96 samples within 70 min (eight pipettes)
• Ready to use system
• Low tip throughput
• Sample identification possible
• Low cost solution

Capacity and Throughput

The Xiril robotic workstations are easily configured to meet different demands for capacity and throughput. Two deck sizes (100-series and 150-series) and the option of four or eight pipettes provide the flexibility to readily meet your requirements. Refer to table 1 below for approximate throughputs for 96 samples for two different instrument configurations.

Table 1: Throughputs for 96 samples for the 100-series instruments with 4 or 8 pipettes.

Instrument                                                                  Run duration [minutes]

---------------------------------------------------------------------------------------------------------------------------

100-series, 1 arm, 4 pipettes                                     115

100-series, 1 arm, 8 pipettes                                    70

Automated Processing Requirements for the 100-series with One Robotic Arm and Four Pipettes

XIRIL ROBOTIC WORKSTATION DECK SETUP

This is an example of DNA extraction deck layout on a 100-series-1-4 robotic workstation for use with one plate (96 samples). The layout can be easily configured to user’s needs with the flexibility to position hardware and labware freely on the instrument deck. The samples can be hold in different containers such as 96-well microplate, Eppendorf tubes or sample tubes. Furthermore if needed the sample identification and tracking can be enabled by the following methods: The import of a sample file (e.g. LIMS), which is implemented in the Lirix software, a handheld barcode reading, plate scanning and finally tube scanning. Please refer to the figure below for the recommended deck layout.

Figure 1: Deck layout for DNA extraction on the Xiril 100-1-4 robotic workstation.

1: Module Decktray,

2: Tip waste station and liquid waste,

3: Re-Use rack for tips,

4: Plate-GripX,

5: Thermo­ShakeX,

6: High Precision Decktray,

7: MagBeadX,

8: High Precision Decktray,

9: Reagent rack incl. 6 troughs,

10: Reagent rack,

11: 250 µl Rainin filter tips,

12: Standard Decktray,

13: 96-well microplate,

14: Module Decktray,

15: Rack for 32 microfuge tubes.