Firma:                Organon

Modell:              Nuclisens

Kommentar:      Dokumente engl.

INTRODUCTION

The NucIiSens System consists of the Nucleic Acid Sequenced Base Amplification (NASBA) technology combined with a simple nucleic acid extraction procedure, NucIiSens Extractor, and a user-friendly detection method, NucIiSens Reader. The NucIiSens Extractor automates the Isolation process for the nucleic acids. This instrument relieves the user of the many centrifugation, suspension, and aspiration steps. It allows the processing of multiple samples during a single Run, minimizes the labor and reduces the time required for the processing steps.

INTENDED USE

The NucIiSens Extractor is an instrument for the extraction of nucleic acids from lysed biological samples; the nucleic acids are concentrated by adsorbing to Silica, filtering, washing, and eluting into a closed vial for further processing.

The extraction and concentration of the nucleic acids are performed in a closed container, referred to as the nucleic acid extractor Cartridge. The Cartridge is designed to hold the lysed biological sample, Silica, and internal control nucleic acid (if used), during the extraction process. A ready source of filtered, dry air provided to the Cartridge allows fluid manipulation for the filtration, washing and elution steps, and for drying of the Silica following the wash steps. This minimizes contamination of the nucleic acids and exposure of the operator to hazardous biological samples.

GENERAL THEORY OF OPERATION

The 'Boom" method utilizes the lysing properties of a chaotropic agent and the nucleic acid binding properties of a solid phase, Silica, in the presence of this agent.

The extraction procedure is initiated by adding the biological sample to Lysis Buffer. The Lysis Buffer disrupts (dissociates) the cells and/or viral particles, releasing the nucleic acids and inactivating the nucleases present in the mixture. Silica is added to the lysed mixture for adsorption of the nucleic acids prior to processing by the Extractor. (When calibrator or control nucleic acids are used, these materials are added prior to the addition of Silica.) This mixture, containing sample (nucleic acids), Lysis Buffer, and Silica, is added to the Cartridge which is subsequently placed on the Extractor for processing. The Silica (solid phase) containing the adsorbed nucleic acids is filtered from the mixture, washed to remove extraneous materials, and dried. The adsorbed nucleic acids are eluted from the Silica into a closed Eluate Cup for further processing.

EXTRACTOR SPECIFICATIONS