Firma:              Perkin Elmer

Modell:                         Lambda 14

Kommentar:     Dokumente engl.

Lambda 14 is a versatile spectrometer operating in the ultraviolet (UV) and visible ( Vis ) spectral ranges. The spectrometer features a double-beam, all-reflecting optical system, with four selectable fixed slit widths.

Lambda 14 is usable in a wide range of applications as indicated by its performance specifications.

Technical Data

General

Type                               Scanning double-beam spectrometer for the UV/Vis range; with                                       microprocessor and keyboard.

Dimensions                    Width: 650 mm

                                      Height: 233 mm

                                      Depth: 560 mm

Mass                              26.4 kg approx.

Power requirements       100 V to 240 V AC, 50/60 Hz; 300 VA

Ambient operating          15° C to 35° C

temperature

Humidity range               20% to 80% relative humidity without condensation

Technical Standard        In compliance with the requirements for technical instruments                                       stipulated by IEC 1010-1/9.90.

Radio interference         In compliance with the legal requirements of

suppression                   the FRG (VDE 0871 grade B)

Optics

Beam center height        15 mm above cell holder bottom.

Beam Cross-section      0.5 nm slit      ca. 0.25 mm x 7 mm

                                                           ( width x height)

                                      1 nm slit         ca. 0.5 mm x 7.5 mm

                                                           ( width x height),

                                      2 nm slit         ca. 1 mm x 7.5 mm

                                                           ( width x height),

                                      4 nm slit         ca. 2 mm x 7.5 mm

                                                           ( width x height),

                                                           at focal point of sample and reference beam in sample                                                            compartment.

Optical pathlength in      121 mm

sample compartment

Grating                           Holographic concave grating with

(Monochromator)           1053 lines/mm in the center.

Radiation sources          Prealigned deuterium and halogen lamps.

Detector                        Photodiodes

                                      (One for the sample beam and one for the reference beam)

Abscissa

Wavelength range          190 nm to 1100 nm

Wavelength accuracy    + 0.3 nm

Wavelength                    0.1 nm reproducibility

Spectral bandwidth        0.5 nm, 1 nm, 2 nm, 4 nm (selectable fixed slit widths) Lamp change                                       automatically at 326 nm

Scan speeds                  7.5, 15, 30, 60, 120, 240, 480, 960, 1920 and 2880 nm/min

Ordinate

Photometric range         Transmission 0% to 100%

                                      Absorbance -6.000 to 6.000 (Display range)

                                      1 to 9999 (concentration units)

Photometric accuracy    Absorbance ± 0.003

                                      ( measured at Absorbance = 1, at 440 nm, 546.1 nm and 635 nm with                                       NIST 930 filters)

                                      Absorbance ± 0.015

                                      ( measured at Absorbance = 1, at 257 nm and 350 nm with potassium                                       dichromate solution)

Stray radiation                Transmission < 0.02%

                                      ( at 220 nm, 340 nm and 370 nm)

                                      Absorbance > 2

                                      ( measured at 200 nm with potassium chloride solution against                                       distilled water)

Baseline linearity            2 nm slit:        Absorbance ± 0.0005

                                      ( corrected ; 200 nm to 1100 nm, scan speed 240 nm/min, smooth 2)

Baseline noise               2 nm slit:

                                      Absorbance < 0.00006 RMS,

                                      Absorbance < 0.0002 peak-to-peak

                                      (3 min at Absorbance = 0, wavelength 500 nm and response 2 s)

Baseline stability            Absorbance < 0.0003 per hour

( drift )                              (500 nm, after warmup)

Data Output

Digital port                      One RS 232 C interface (serial), for connecting a printer or PC;                                       optional second RS 232 C interface.

Display                           Information, parameters and entries are shown on a two line vacuum                                       fluorescence display with 20 alphanumeric characters per line.

Methods

Types                             Time drive, Scan, Wavelength Program, Concentration 1 and                                       Concentration 2 Analysis with factor and with Calibration, Oligoquant 1,                                   Oligoquant 2, Enzyme Kinetics, Substrate Kinetics.