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Usually, laboratory and analysis devices are very exigent system with a very own specifications and requirements. Therefore, the startup of a laboratory device often rises the question how to assemble, adjust and to launch it.
We would be pleased to take over the professional installation of your laboratory devices!Please contact us.
Benefit from the installation by us in more than one way:
24 months legal warranty
60 days Labexchange Fullguarantee
Certificate of operativeness
The Labexchange right of return
Optional installation of equipment for immediate and safe use of equipment
Questions regarding this device
If you buy this item used instead of new, you save as much CO2 as driving 3610 km with a gasoline car.
The legal warranty period for our devices is 24 months from delivery date.
All used instruments of Labexchange are subject to a technical inspection before delivery and will be supplied only, when they function perfectly well. The verification and functionability is documented by a service report of an independent service company. The Labexchange Fullguarantee is 60 days from delivery date. Malfunctions occurring within the warranty period will be corrected by us free of charge. (Valid for countries within the EU and CH)
Our certificate of operativeness ensures a technically perfect condition.
You are on the safe side with our used laboratory and analysis systems! For even more security during the purchase, we are granting the Labexchange right of return.
Return your laboratory or analysis system simply without explanation.
Our special terms and conditions are to be applied:Labexchange right of return(https://www.labexchange.com/fileadmin/user_upload/pdf/downloads/en/Rueckgaberecht_08_18_E.pdf)
On demand, we verify the option of an extension of warranty for your device. If possible, the period of warranty is 6 months for 10 % extra charge and 1 year for 20 % extra charge on the list price. (Valid for DE, AT, CH, FR, NL, BE, LU, GB and IR)
Play it safe! We are pleased to offer you the installation of your laboratory equipment. Our qualified technicians install the device on your desired date in the laboratory, so that you can work quickly and safely with your new laboratory equipment.
The installation of the equipment by Labexchange brings further advantages. You thereby obtain a guarantee extension to 6 months and you have also the right to the Labexchange Repurchase Guarantee. You receive sensible laboratory equipment and analytical systems as well as much of the available laboratory hoods, already incl. installation.
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The second-hand devices are verified by Labexchange Service GmbH before delivery. You are receiving only fully functional devices.
The stated dispatch times are the shortest possible ones for each article. The effective dispatch times can vary. The effective dispatch times will be stated in the order confirmation.
As a matter of principle, we are offering collective deliveries. The shipping time is calculated based on the position with the longest lead time. A partial delivery is possible on explicit request.
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For deliveries to countries outside the EU, an additional fee of 70,00€ for export is charged. Note for international shipments: A proof of preference/EUR1 will not be issued by us. When self-collecting/ordering EXW from countries within or outside the European Union, 16% VAT will be retained as a deposit until we have received the corresponding confirmation of arrival/bill of delivery from the buyer.
We do not accept payment by credit card, letter of credit, PayPal, etc. In each case the invoice amount is payable without deduction. Discount is not granted.
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DE, AT, CH
Payment by invoice, payment in advance
Payment by invoice is only possible for corporate clients.
NL, BE, LU
Payment by invoice is only possible for corporate clients
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The goods are offered subject to prior sale.
All articles are used articles, except an article is listed especially as a new device.
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Used
The article is on stock. Our service technicians will verify the article before delivery. You are receiving only a fully functional article.
Published
The article is still with the provider. After your order the article will be purchased and verified by us before being shipped to you. A certificate of operativeness as well as a service report are included in delivery.
New device
new
The article is brand new and unused. Regarding new equipment the guarantee/warranty conditions of the corresponding manufacturer apply.
manufacturer :
model :
annotation : Dokumente engl .
The following illustrations and descriptions refer to the instrument model and are drawn from brochures. They do not represent the scope of delivery. Please refer to the text of the offer for the exact scope of delivery.
Overview of the LCT Premier
The LCT Premier TM time-of-flight (TOF) mass spectrometer features a ZSpray source with electrospray ionization (ESI) and modular LockSpray TM interface.
The LCT Premier uses ion guide technology for optimum transfer of ions from the source to the orthogonal acceleration time of flight ( oa -TOF) mass analyzer. Ion detection is by dual microchannel plate detector assembly. Data is acquired by a 4.0 GHz time-to-digital converter (TDC) and histogrammed in an embedded PC. Instrument control and data acquisition is by the MassLynx TM software system.
lonization Techniques
Using the Zspray atmospheric pressure ionization (API) source, three techniques are available.
ElectroSpray lonization
Atmospheric Pressure Chemical lonization
Atmospheric Pressure Photoionization
Performance Specifications
Resolution in Positive Ion (V Mode)
Infuse mellitin at a concentration of 5 pmol /µL, in 50:50 acetonitrile :water + 0.2% formic acid, using a flow rate of 5 µL/min. Ensure that the intensity of the peak at 712 Da is <300 counts/s.
The peak at 712 Da should be resolved such that the mass divided by the peak width at half height is greater than 5000 when using a scan time of 1 s and an inter-scan delay of 0.1 s. At least 30 s of data should be summed.
Resolution in Positive Ion (W Mode)
Infuse mellitin at a concentration of 5 pmol /µL in 50:50 acetonitrile :water + 0.2% formic acid, at a flow rate of 5 µL/min. Ensure that the intensity of the peak at 712 Da is <300 counts/s.
The peak at 712 Da should be resolved such that the mass divided by the peak width at half height is greater than 10,000 when using a scan time of 1 s and an inter-scan delay of 0.1 s. At least 30 s of data should be summed.
Resolution in Negative Ion (V Mode)
Infuse raffinose at a concentration of 500 pg/µL in 50:50 acetonitrile :water , at a flow rate of 10 µL/min. Ensure that the intensity of the peak at 503 Da is <300 counts/s.
The peak at 503 Da should be resolved such that the mass divided by the peak width at half height is greater than 5,000 when using a scan time of 1 s and an inter-scan delay of 0.1 s. At least 30 s of data should be summed.
Resolution in Negative Ion (W Mode)
Infuse raffinose at a concentration of 500 pg/µL in 50:50 acetonitrile :water at a flow rate of 10 µL/min. Ensure that the intensity of the peak at 503 Da is < 300 counts/s.
The peak at 503 Da should be resolved such that the mass divided by the peak width at half height is greater than 10,000 when using a scan time of 1 s and an inter-scan delay of 0.1 s. At least 30 s of data should be summed.
Sensitivity in Positive Ion
In V mode, infuse 50 pg/µL of leucine enkephalin in 50:50 acetonitrile :water + 0.1% formic acid at a flow rate of 5 µL/min. Using a scan rate of 1 s, an inter-scan delay of 0.1 s and a mass range of 100 to 1000 Da , check that the resolution at 556 Da is greater than 5000 and the intensity is greater than 440 counts/s.
Switch the instrument to W mode. Check that the resolution is greater than 10,000 and the intensity is greater than 110 counts/s .
Sensitivity in Negative Ion
In V mode, infuse 500 pg/µL. of raffinose in 50:50 acetonitrile :water at a flow rate of 10 µL/min. Using a scan rate of 1 s, an inter-scan delay of 0.05 s and a mass range of 100 to 1000 Da , check the resolution at 503 Da is greater than 5000 and the intensity is greater than 240 counts/s.
Switch the instrument to W mode. Check that the resolution is greater than 10,000 and the intensity is greater than 60 counts/s .
Mass Calibration Accuracy
Infuse 50 pg/µL of sodium formate at a flow rate of 5 µL/min into the analyte probe.In V mode, ensure that the intensity is less than 300 counts/s and that the resolution is greater
than 5000. Run a calibration using the parameters listed in Table E-1, with no smoothing and no background subtraction, and using a centroid top value of 80%.
Table E-1 Mass Calibration Accuracy Parameters
Scan Type Continuum
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Scan Range 150-900 Da
Scan Time 5 s
Inter-Scan Delay 0.1 s
Acquisition Time 1 min
Save the calibration and then perform an acquisition for 1 minute using the same parameters. Combine and center the data, using 4 channels and a centroid top value of 80%.Measure at least 6 peaks and calculate the mass error ( ppm ) for each peak. Using the formula below, calculate the RMS error. The RMS error should be < 5 ppm .
Mass Measurement Accuracy
Infuse 50 pg/µL of sodium formate at 5 µL/min into the analyte probe . In W mode, ensure that the intensity is less than 300 counts/s and that the resolution is greater than 10,000. Run a calibration using the parameters listed in Table E-1. Create a calibration with no smoothing and no background subtraction, using a centroid top value of 80%.Save the calibration and then perform the following acquisition.
Infuse 50 pg/µL of leucine enkephalin into the reference probe and 500 pg/µL of raffinose into the ESI probe. Ensure that both probes give an intensity of <200 counts/s and that the resolution is greater than 10,000.
Acquire data in LockSpray mode for 25 minutes, using the following parameters:
Scan Range 100-1000 Da
Scan Time 1 s
Acquisition Time 25 min
Reference Scan Frequency 5
Reference Cone Voltage 100 V
Combine 12 scans at each of the following times: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20 minutes.
Using the Mass Measure feature an each of the spectrums, create a centered spectrum using lockmass 556.2771 Da . Calculate the RMS error using the formula below. The m/z value of raffinose is 527.1588 Da .
The calculated RMS error should be <3 ppm .
Chromatographic Signal to Noise
Note: This test should only be performed if there is already a suitable HPLC system installed.
Infuse 50 pg/µL of leucine enkephalin at a flow rate of 5 µL/min. Tune the ion beam in V mode and check that the resolution is greater than 5000.
Purge the HPLC using 75:25 methanol :water and 5 mM ammonium acetate. Connect the 10-µ.L sample loop, injection port, and waste tube to the Rheodyne injector. Connect a Waters Atlantis dC18 column (2.1 x 30 mm; 3 µM) between the injector and the probe. Condition the column for approximately 20 minutes.
Set up the following sample list acquisition parameters:
Table E-2 Sample List Acquisition Parameters
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Scan Time 2 s
Enter-Scan Delay 0.I s
Acquisition Time 30 min
Make five injections of 1 pg/µL reserpine in 75:25 methanol :water and 5 mM ammonitun acetate, at 5 minute intervals.
Using the 609 Da mass chromatogram, calculate the peak to peak, 1 SD signal to noise ratio for each peak. The average signal-to-noise value should be >100.
Your Labexchange-Team will gladly help:
Christian Schmid Laboratory and analysis, Laboratory equipment, Life science
Hubert Sauter Spectroscopy, Chromatography
